Amelioration of Ultraviolet-B-lnduced Down-Regulation of mRNA Levels for Chloroplast Proteins, by High Irradiance, is Mediated by Photosynthesis

Mackerness, Soheila A.-H.; Butt, P. Joe; Jordan, Bill; Thomas, Brian

doi:10.1016/s0176-1617(96)80300-2

Cited by 32 publications

(13 citation statements)

References 22 publications

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“…It was higher in LL than in HL culture (Fig. 5), confirming previous findings that HL may alleviate to a certain extent the UV‐B injury ( 15–19 ). Another explanation of the protective role of HL against the UV‐B induced damage on the photosynthetic apparatus concerns the degree of photoadaptation to PAR intensity of the cultures exposed to UV‐B and is based on the experimental data presented in this work.…”

Section: Discussionsupporting

confidence: 89%

Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes^¶

Sfichi

Loannidis

Kotzabasis

2004

Photochem & Photobiology

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The sensitivity of the photosynthetic apparatus to ultraviolet‐B (UV‐B) irradiation was studied in cultures of unicellular green alga Scenedesmus obliquus incubated in low light (low photosynthetically active radiation intensity [LL]) and high light (high photosynthetically active radiation intensity [HL]) conditions, treated or not with exogenous polyamines. Biochemical and physicochemical measurement showed that UV‐B radiation induces a decrease in the thylakoid‐associated putrescine (Put) and an increase in spermine (Spm), so that the reduction of Put/Spm ratio leads to the increase of light‐harvesting complex II (LHCII) size per active reaction center and, consequently, the amplification of UV‐B effects of the photosynthetic apparatus. The separation of oligomeric and monomeric froms of LHCII from isolated thylakoids showed that UV‐B induces an increase in the oligomeric forms of LHCII, which was more intense in LL than in HL. By manipulating the LHCII size with exogenous polyamines, the sensitivity degree of the photosynthetic apparatus to UV‐B changed significantly. Specifically, the addition of Put decreased highly the sensitivity of LL culture to UV‐B because of the inhibitory effect of Put on the LHCII size increasing, wheres the addition of Spm enhanced the UV‐B injury induced in HL culture because of the increasing of LHCII size. The ability of the photosynthetic apparatus to recover the UV‐B induced changes was also investigated.

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Section: Discussionsupporting

confidence: 89%

Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes^¶

Sfichi

Loannidis

Kotzabasis

2004

Photochem & Photobiology

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“…Such a notion is strengthened by the fact that higher photosynthetically active radiation has been shown previously to partially protect against stress in UV‐B‐irradiated peas ( Jordan et al 1991 ; Strid et al 1996 ; A.‐H. Mackerness et al 1996 ).…”

Section: Discussionmentioning

confidence: 97%

“…The differences in the development of leaf lesions between plants grown outdoors and indoors could be due to the fact that the former also were given a higher dose of photosynthetically active radiation for a longer period of time during the Scandinavian summer. Such a notion is strengthened by the fact that higher photosynthetically active radiation has been shown previously to partially protect against stress in UV-Birradiated peas (Jordan et al 1991;Strid et al 1996;A.-H. Mackerness et al 1996).…”

Section: General Considerationsmentioning

confidence: 95%

Molecular markers for ozone stress isolated by suppression subtractive hybridization: specificity of gene expression and identification of a novel stress‐regulated gene

Sävenstrand¹,

Brosché²,

Ängehagen³

et al. 2000

Plant Cell & Environment

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Suppression subtractive hybridization was used to identify genes regulated by ozone (100 nmol mol -1 ) in Pisum sativum. One novel gene (named PsUod1) was found. In addition, mRNA levels for four genes (encoding lipid transfer protein, pre-hevein-like protein, leucine-rich repeat protein, and disease-resistance response protein 230), which previously were shown to be regulated by biotic stress, increased. Finally, mRNA species for two genes (encoding extensin and pathogenesis-related protein 4A), previously shown to be regulated by ozone in other species, were found to increase in abundance. The ozone-specificity of the expression of these genes was studied by using UV-B radiation. PsUod1 and the genes encoding extensin, leucine-rich repeat protein, and disease-resistance response protein 230, were differentially regulated when comparing ozone and UV-B. Moreover, the mRNA levels for extensin, leucine-rich repeat protein and disease-resistance response protein 230 all increased under NaCl and aluminium stress and after wounding, whereas the message abundance for PsUod1 was unchanged under these stresses. Thus, in general, ozone caused changes similar to wounding, salt stress and aluminium stress, whereas UV-B radiation regulated gene expression differently.

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“…This may be connected with its interaction with UV-B signal transduction and gene expression. Mackerness et al (1996) showed that amelioration of UV-B effects on gene expression by strong irradiation involved photosynthetic electron transport and photophosphorylation. This may, in part, account for the lack of UV-B effect on gene expression in etiolated tissue when photosystems are not functional.…”

Section: Discussionmentioning

confidence: 99%

UV-B response of green and etiolated barley seedlings

Fedina¹,

Velitchkova²,

Georgieva³

et al. 2007

Biologia plant.

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7-d-old etiolated and green barley seedlings (Hordeum vulgare L. cv. Alfa) were irradiated with UV-B for 30 min and then kept for 24 h in light or darkness. Chlorophyll (Chl) synthesis was inhibited by about 30 % as a result of UV-B irradiation, but there were no significant changes in photochemical activity measured by variable to maximum fluorescence ratio (F v /F m ), quantum yield ( PS2 ) and oxygen evolution rate. Electron transport of etiolated seedlings was similar to that of green ones, nevertheless, the Chl content was more then 2-fold lower. Ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits were diminished as a result of UV-B irradiation in etiolated and green plants, especially in those kept in the darkness. Catalase activity decreased and total superoxide dismutase activity increased in green and etiolated plants following UV-B treatment. When benzidine was used as a substrate, an isoform located between guaiacol peroxidases 2 and 3 (guaiacol peroxidase X) appeared, which was specific for UV-B treatment. As a result of irradiation, the contents of UV-B absorbing and UV-B induced compounds increased in green seedlings but not in etiolated seedlings.Additional key words: chlorophyll fluorescence, flavonoids, Hordeum vulgare, oxygen evolution, ribulose-1,5-bisphosphate carboxylase/oxygenase.

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Amelioration of Ultraviolet-B-lnduced Down-Regulation of mRNA Levels for Chloroplast Proteins, by High Irradiance, is Mediated by Photosynthesis

Cited by 32 publications

References 22 publications

Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes^¶

Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes^¶

Molecular markers for ozone stress isolated by suppression subtractive hybridization: specificity of gene expression and identification of a novel stress‐regulated gene

UV-B response of green and etiolated barley seedlings

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Amelioration of Ultraviolet-B-lnduced Down-Regulation of mRNA Levels for Chloroplast Proteins, by High Irradiance, is Mediated by Photosynthesis

Cited by 32 publications

References 22 publications

Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes¶

Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes¶

Molecular markers for ozone stress isolated by suppression subtractive hybridization: specificity of gene expression and identification of a novel stress‐regulated gene

UV-B response of green and etiolated barley seedlings

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Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes^¶

Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes^¶