Ameliorative Effect of Curcumin Nanoparticles against Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats

Abstract

Aim. This study is aimed at evaluating the use of curcumin-loaded polylactic-co-glycolic acid nanoparticles (CUR-loaded PLGA NPs) as a treatment against monosodium iodoacetate- (MIA-) induced knee OA. Materials and Methods. Eighteen rats were assigned to three groups ( n = 6 ), namely, normal control group that received intra-articular injections (IAIs) of saline, an OA control group that received an IAIs of MIA (2 mg/50 μL), and a treatment group (MIA+CUR-loaded PLGA NPs) that received IAIs of CUR-loaded PLGA NPs (200 mg/kg b.wt). Results. The CUR NP treatment against knee OA alleviated radiographic alternations and histopathological changes and inhibited the upregulation in the serum levels of interleukin-1β, tumor necrosis factor-α, interleukin-6, and transforming growth factor-beta and the downregulation in interleukin-10. CUR NP-treated joints also decreased the mRNA expression of nuclear factor-kappa B and inducible nitric oxide synthase and the protein expression of matrix metalloproteinase-13 and caspase-3. Finally, CUR-loaded PLGA NP treatment mitigated the loss of type II collagen, which resulted in a significant reduction in malondialdehyde level and increased the glutathione content and superoxide dismutase activity compared with that of the OA group. Conclusion. This study demonstrated that the administration of CUR NPs could provide effective protection against MIA-induced OA and knee joint histological deteriorated changes due to its anti-inflammatory, antioxidant, and antiapoptotic properties.

L-Carnitine augments probenecid anti-inflammatory effect in monoiodoacetate-induced knee osteoarthritis in rats: involvement of miRNA-373/P2X7/NLRP3/NF-κB milieu

“…In addition to sub-synovial tissue fibrosis, synovial hyperplasia was seen. There were collections of mixed lymphoplasmacytic infiltration and large congested vascular areas (Hamdalla et al 2022 ; Johnson et al 2022 ). Also, the total score given upon assessment of the synovial membrane of knee joint H and E stained sections was significantly increased in OA rats as compared to normal rats, indicating the occurrence of hyperplasia and inflammation.…”

Therapeutic effects of combining curcumin and swimming in osteoarthritis using a rat model

“…One week later, OA rats (group 2) were given oral saline for a total of 4 weeks. Meanwhile, the 20-minutes swimming sessions were introduced to rats of groups 3 and 5 and curcumin was orally administered to the 4th and 5th groups in a dose of 200 mg/kg [ 25 ] till the end of the experimental period (35 days).…”

“…Rats were anaesthetized using ketamine (50 mg/kg)/xylazine (2 mg/kg) mixture and positioned with hind limbs straightened by an adhesive tape. Imaging of the lateromedial and craniocaudal views of the right stifle were obtained using a Fisher ® X-ray device (Fisher R183, Emerald tube 125) [ 25 ].…”

Collagen type X expression and chondrocyte hypertrophic differentiation during OA and OS development

“…The MIA-induced OA model has also been applied to the treatment of OA [95,96]. Curcumin nanoparticles can improve the progression of MIA-induced OA in rats, and the content of MMP13 in articular cartilage is significantly increased in OA models [97]. p53 is a key factor in OA pain.…”