Ameliorative effect of polydatin on oxidative stress-mediated testicular damage by chronic arsenic exposure in rats

Abstract

Arsenic causes lipid peroxidation leading to alterations in antioxidant status in organisms. In this study, the reproductive effects of chronic exposure to arsenic and the protective effects of polydatin (PD) were evaluated in 35 Wistar male rats, which were divided equally into five groups. The control group received a normal diet and tap water, arsenic (100 mg l(-1) , approximately 1/50 of oral LD50 ) was given via drinking water to experimental groups except control group, and PD was orally given to the other groups at dose of 50, 100 and 200 mg kg(-1) for 60 days. Arsenic administration decreased sperm motility, glutathione level, superoxide dismutase and catalase activities in testicular tissue of rats. In contrast, malondialdehyde level and DNA damage were found to be high levels in arsenic-treated group. Histopathologically, it was observed that decreased sperm concentration and degeneration of Sertoli cells in testicular tissue. PD administration, partially 200 mg kg(-1) , reversed arsenic-induced lipid peroxidation, DNA damage, antioxidant enzyme activity and cell integrity in testis of rats. These results demonstrate that PD decreases arsenic-induced lipid peroxidation, enhances the antioxidant defence mechanism and regenerates tissue damage in testis of rats.

Polydatin inhibits hepatocellular carcinoma via the AKT/STAT3‑FOXO1 signaling pathway

“…The results of the present study indicated that the levels of phosphorylated AKT, JAK1 and STAT3 were markedly decreased following treatment of HCCML3 cells with polydatin; however, no change was observed in p38 and p-p38 expression. The protein p38 is involved in oxidative stress regulation, suggesting that polydatin may not increase intracellular reactive oxygen species, which is consistent with the findings of previous studies (38,39). FOXO1 belongs to the forkhead family and serves numerous roles, including cellular differentiation, proliferation, cell cycle progression, apoptosis and glucose metabolism (40).…”

α‐Lipoic acid inhibits testicular and epididymal oxidative damage and improves fertility efficacy in arsenic‐intoxicated rats

“…Further, reduced reproductive organ weights and the disorganization of testicular and cauda epididymal architecture could be attributed to the inadequate supply of androgens . We found a significant reduction in the activity levels of SOD [EC: 1.15.1.1], CAT [EC:1.11.1.6], and GSH levels with a concomitant increase in the LPx levels in the testis and the cauda epididymis of arsenic‐treated rats suggesting accumulation of superoxides and peroxides with altered glutathione system . Moreover, accumulation of protein carbonyls may suggest protein oxidation in the selected tissues of arsenic‐treated rats…”

Antioxidative stress of oral administration of tea extract granule in chickens

“…Catalase is the most important cellular antioxidant. It can degrade O 2 and decompose H 2 O 2 and result in a decrease in oxidative stress, which is the effective way of protection from ROS attack ( Ince et al., 2014 ). Glutathione peroxidase is an enzyme involved in scavenging the ROS and protecting the body from LPO by reducing both lipidic and nonlipidic hydroperoxides as H 2 O 2 ( Avanzo et al., 2001 ).…”