Antioxidative stress of oral administration of tea extract granule in chickens

Abstract

The present study was to evaluate antioxidative effect of tea extract granule ( TEG ) on oxidative stress induced by cyclophosphamide ( Cy ) in chickens. In experiment 1, chickens were randomly divided into 5 groups with 10 birds in each. Groups 3 to 5 were orally administered TEG in drinking water for 7 D at doses of 20, 40, and 80 mg/kg body weight, respectively. After that, groups 2 to 5 received intramuscular injection of Cy (100 mg/kg BW) for 3 D. Group 1 was not treated as a control. In experiment 2, chickens were grouped in the same way as in experiment 1. Groups 2 to 5 received intramuscular injection of Cy (100 mg/kg BW) for 3 D. After that, groups 3 to 5 were orally administered TEG in drinking water for 7 D at doses of 20, 40, and 80 mg/kg BW, respectively. Results showed that Cy injection induced significantly decreased body weight and oxidative stress. Oral administration of TEG before or after Cy injection increased body weight, the thymus, bursa, and spleen indices, total antioxidant capacity, and the levels of glutathione; elevated the activity of superoxide dismutase, catalase, and glutathione peroxidase; as well as decreased the protein carbonyl content, lipid peroxide, and malondialdehyde. In addition, TEG administration reduced intracellular reactive oxygen species. Therefore, TEG could be a promising agent against oxidative stress in the poultry industry.

Proteome and Transcriptome Analysis of the Antioxidant Mechanism in Chicken Regulated by Eucalyptus Leaf Polyphenols Extract

“…Similarly, MDA content can reflect the degree of lipid peroxidation; thereby, indirectly reflect cell damage and freshness of meat [41]. In agreement with our results, a diet with green tea extract led to a remarkably decreasing MDA content in meat tissue [42]. Polyphenols might be accumulated to exhibit antioxidant effects in meat tissues [35].…”

Protective Effect of Epigallocatechin-3-Gallate in Hydrogen Peroxide-Induced Oxidative Damage in Chicken Lymphocytes

“…Chi et al observed that oral administration of tea saponins significantly increased T-AOC, SOD, CAT, GSH-PX, GSH, VC, and VE and enhanced the immune responses to Newcastle disease and infectious bronchitis vaccines in chickens with oxidative stress induced by cyclophosphamide [ 41 ]. They recently found that oral administration of tea extract granule (TEG) significantly increased T-AOC, SOD, CAT, GSH-PX, and GSH as well as decreased Carbonyl, LPO, and MDA [ 58 ]. EGCG has a characteristic structure with a large number of hydroxyl groups.…”

Tea tree oil inhibits hydrogen sulfide-induced oxidative damage in chicken lungs through CYP450s/ROS pathway

“…The homogenate was mechanically homogenized under the condition of ice water bath. The homogenate was centrifuged at 3,000 r/min for 10 min, and the supernatant was stored in a refrigerator at -20℃ until measured ( Chi et al, 2020 ). The concentrations of TP, ROS, T-AOC, CAT, MDA, SOD, H2O2 and GSH-Px were determined according to the instructions of the kit manufacturer.…”