Ameliorative effects of Artemisia argyi Folium extract on 2,4-dinitrochlorobenzene-induced atopic dermatitis-like lesions in BALB/c mice

Abstract

Artemisia argyi Folium has been used to treat skin diseases, including eczema and dermatitis, in South Korean medicine. The present study investigated the curative effects of Artemisia argyi Folium extract (AAFE) on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in a BALB/c mouse model. Briefly, the dorsal skin of the BALB/c mice was sensitized three times with DNCB, whereas the ears were challenged twice. Repeated treatment with DNCB induced AD-like lesions. The effects of AAFE on AD-like lesions were evaluated by clinical observation, histopathological analysis, immunohistochemistry and enzyme-linked immunosorbent assay. In addition, reverse transcription-polymerase chain reaction and western blotting were performed. Treatment with AAFE reduced AD-like lesions, as determined by clinical observation, histopathological analysis, and detection of the serum levels of histamine, immunoglobulin E and cytokines. With regards to its mechanism of action, AAFE inhibited the phosphorylation of Lck/yes-related novel tyrosine kinase (Lyn), spleen tyrosine kinase (Syk), mitogen-activated protein kinases (MAPKs), phosphoinositide 3-kinase (PI3K)/Akt and IκBα, which have essential roles in the production of various cytokines in lymph nodes. The suppressive activity of AAFE may be due to the inhibition of a series of immunopathological events, including the release of proinflammatory cytokines. The results of the present study strongly suggest that AAFE exerts an anti-AD effect by inhibiting the Lyn, Syk, MAPKs, PI3K/Akt and IκBα pathways. Therefore, AAFE may be considered an effective herbal remedy for the treatment of AD.

The Essential Oil of Artemisia argyi H.Lév. and Vaniot Attenuates NLRP3 Inflammasome Activation in THP-1 Cells

“…Artemisia argyi H. Lév. and Vaniot extract from different research groups showed significant inhibitory effect on dermatitis related lesions ( Han et al, 2016 ; Yun et al, 2016 ). Artemisia argyi H. Lév.…”

Water extract of Artemisia ordosica enhances antioxidant capability and immune response without affecting growth performance in weanling piglets

“…Bits of cytokines may serve as cell‐signalling molecules, which trigger a disease‐fighting response from nuclear factor of kappa B (NF‐κB) signal pathway, hence promoting the formation of immunoglobulins (Epinat & Gilmore, ) and cytokines, such as IL‐1, IL‐2, IL‐6, TNF‐α, as well as growth factor (Huang & Miyamoto, ). As reported, the Artemisia argyi extract ameliorated inflammation by regulating IκBα/NF‐κB pathways (Han et al, ; Ryu et al, ; Zhang et al, ). However, the effects of WEAO on the NF‐κB signalling pathway were not investigated in this study, and further research is still needed.…”

“…() indicated that consumption of Artemisia ordosica aqueous extract was sufficient to relieve the immune stress imposed by lipopolysaccharide in broiler chickens. Artemisia argyi folium extracts have been shown the regulating effects on the immune system in mice model as well (Han et al, ). Furthermore, in this study, we found there was significant increase in serum IgG, IgA and IgM in piglets with consumption of WEAO diets, which is similar to the previous reports showing that the serum levels of IgG (Sani et al, ) and IgA (Song et al, ) were markedly increased in animals feeding diets supplemented with Artemisia annua .…”

Caffeoyl-Prolyl-Histidine Amide Inhibits Fyn and Alleviates Atopic Dermatitis-Like Phenotypes via Suppression of NF-κB Activation

“…In addition, polydatin (3, 4′, 5-trihydroxystilbene-3-β-mono-D-glucoside), a natural component extracted from Polygonum cuspidatum , directly inhibited activity of SYK and suppressed pro-inflammatory cytokine expression in mast cells [ 75 ]. Extracts of Cymbidium eburneo-kanran and Artemisia argyi down-regulated the expression of pro-inflammatory cytokines through suppression of the signaling pathway, including phosphorylation of SYK [ 50 , 76 , 77 ]. The extracts further alleviated DNCB-induced AD-like phenotypes, including skin lesion severity, scratching behavior, and IgE levels [ 50 , 76 , 77 ].…”