Ameliorative Effects ofAcaciaHoney against Sodium Arsenite-Induced Oxidative Stress in Some Viscera of Male Wistar Albino Rats

Abstract

Cancer is a leading cause of death worldwide and its development is frequently associated with oxidative stress-induced by carcinogens such as arsenicals. Most foods are basically health-promoting or disease-preventing and a typical example of such type is honey. This study was undertaken to investigate the ameliorative effects of Acacia honey on sodium arsenite-induced oxidative stress in the heart, lung and kidney tissues of male Wistar rats. Male Wistar albino rats divided into four groups of five rats each were administered distilled water, Acacia honey (20%), sodium arsenite (5 mg/kg body weight), Acacia honey, and sodium arsenite daily for one week. They were sacrificed anesthetically using 60 mg/kg sodium pentothal. The tissues were used for the assessment of glutathione peroxidase, catalase, and superoxide dismutase activities, protein content and lipid peroxidation. Sodium arsenite significantly (P < 0.05) suppressed the glutathione peroxidase, catalase, superoxide dismutase activities with simultaneous induction of lipid peroxidation. Administration of Acacia honey significantly increased (P < 0.05) glutathione peroxidase, catalase, and superoxide dismutase activities with concomitant suppression of lipid peroxidation as evident by the decrease in malondialdehyde level. From the results obtained, Acacia honey mitigates sodium arsenite induced-oxidative stress in male Wistar albino rats, which suggest that it may attenuate oxidative stress implicated in chemical carcinogenesis.

The Protective Effects of Insulin and Natural Honey against Hippocampal Cell Death in Streptozotocin-Induced Diabetic Rats

“…At the end of the sixth week, STZ-induced diabetic rats were randomly divided into the following four groups, (each group consisted of 12 rats).

The diabetic group that received no treatment (D).

The diabetic group treated with insulin (I); rats belonging to this group received subcutaneous injections of protamine insulin (NPH) (Exir Boroojerd Pharmacy, Iran). The dose administered was between 3 to 4 units per day.

The diabetic group treated with natural honey (H) (Natural Food Industries of Keshtzar-e Sabz Khansar, Iran); rats in this group received intraperitoneally (IP) daily doses of natural honey (5 mg per kg of body weight) diluted in distilled water [27, 28]. …”

“…The dose administered was between 3 to 4 units per day. The diabetic group treated with natural honey (H) (Natural Food Industries of Keshtzar-e Sabz Khansar, Iran); rats in this group received intraperitoneally (IP) daily doses of natural honey (5 mg per kg of body weight) diluted in distilled water [ 27 , 28 ]. The diabetic group treated simultaneously with insulin and natural honey (I + H); the daily doses of insulin and natural honey were the same as the doses mentioned in “2” and “3,” respectively.…”

The role of molecular modelling strategies in validating the effects of chrysin on sodium arsenite-induced chromosomal and DNA damage

“…All the treatments were orally administered since arsenicals in the environment are majorly ingested through drinking water, and duration of exposure was selected based on our previous works. 3,8,22 However, it is paramount to note that the experiment was carried out in two stages using oral administrations for 2 weeks, the ameliorative stage (stage 1, first week) and the preventive and curative stage (stage 2, extended to second week) as shown in the experimental design below.…”

“…Arsenic is a major environmental toxicant as well as a human carcinogen which is present in large amounts in the environment. 1,2 Arsenicals are found in the environment via industrial, agricultural processes as well as some medical applications, 3 and their consumption through contaminated water is prevalent in many areas of the world. 3,4 They are spontaneously absorbed through gastrointestinal tract after ingestion and distributed in the blood to the liver, kidney, spleen, lung and many other delicate organs.…”

“…1,2 Arsenicals are found in the environment via industrial, agricultural processes as well as some medical applications, 3 and their consumption through contaminated water is prevalent in many areas of the world. 3,4 They are spontaneously absorbed through gastrointestinal tract after ingestion and distributed in the blood to the liver, kidney, spleen, lung and many other delicate organs. 5 Arsenic metabolism in the liver generates reactive oxygen species (ROS) which include dimethyl arsenic peroxyl ([(CH 3 ) 2 AsOO]) and dimethyl arsenic radical [(CH 3 ) 2 As].…”