Amide I Band Analysis Applied to Vibrational Micro-Spectroscopies of Gingival Crevicular Fluid Samples for Orthodontic Treatment Monitoring

Abstract

Vibrational micro-spectroscopies were applied to investigate the gingival crevicular fluid (GCF) for monitoring orthodontic treatment with fixed appliances. The GCF samples were investigated using Fourier transform infrared, Raman, and surface-enhanced Raman micro-spectroscopies. The GCF spectra collected at different times of orthodontic tooth movement were used to characterize the biochemical changes occurring during the treatment. We examined the amide I band region by means of deconvolution analysis using Gaussian–Lorentzian curves for infrared spectra and Lorentzian curves for Raman spectra. This analysis allowed us to evidence the contribution of the different subcomponents of the amide I band and the changes occurring during orthodontic treatment. These changes can be ascribed to modifications in the secondary structure of protein content and could contribute to make vibrational spectroscopies a useful tool for monitoring the individual patient’s response to orthodontic force application.

Validation of the Use of ATR Mode in FT-IR Spectroscopy on Gingival Crevicular Fluid Samples in Orthodontics

“…It has been demonstrated that an unfolding process occurs during the initial stage of orthodontic treatments followed by some recovery during the following days. Modifications could also result from the formation of amyloid aggregates that can be induced by mechanical stress due to the application of orthodontic forces [3].…”

μ-FTIR, μ-Raman, and SERS Analysis of Amide I Spectral Region in Oral Biofluid Samples during Orthodontic Treatment

“…Among a large number of physical and chemical assessment techniques, spectroscopy-based methods are gaining increasing importance thanks to technological progress and the growing knowledge of inherent phenomenology. In this work, we used Fourier Transform Infrared and Raman microspectroscopy ( -FTIR and -RS), and Surface-Enhanced Raman Spectroscopy (SERS) for investigating human gingival crevicular fluid (GCF) samples, as preliminarly reported in a communication at the 8th Electronic Conference on Sensors and Application (ECSA 8) [ 1 ]. Both -FTIR and -RS techniques allow us to extract information on chemical bonds and secondary protein structure from very small quantities of the sample [ 1 , 2 , 3 , 4 , 5 ].…”

“…In this work, we used Fourier Transform Infrared and Raman microspectroscopy ( -FTIR and -RS), and Surface-Enhanced Raman Spectroscopy (SERS) for investigating human gingival crevicular fluid (GCF) samples, as preliminarly reported in a communication at the 8th Electronic Conference on Sensors and Application (ECSA 8) [ 1 ]. Both -FTIR and -RS techniques allow us to extract information on chemical bonds and secondary protein structure from very small quantities of the sample [ 1 , 2 , 3 , 4 , 5 ]. However, the involved mechanisms of these two techniques are different, and complementary information can be obtained.…”