Amikacin Assay in Serum by High-Performance Liquid Chromatography

Maitra, Shyamal K.; Yoshikawa, Thomas T.; Steyn, Catherine M.; Guze, Lucien B.; Schotz, Michael C.

doi:10.1128/aac.14.6.880

Cited by 36 publications

(12 citation statements)

References 13 publications

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“…The assay utilizes direct extraction of the o-phthalaldehyde derivatives from serum and urine. Coefficients of variation were 7.9% (serum) and 6.0% (urine) at a tobramycin concentration of 1.0 ,tg/ml. The lower limit of assay sensitivity was 0.2 ,ug/ml.…”

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confidence: 97%

“…Urinary recovery over the 10-day collection period was greater than 94% of the administered tobramycin dose for two of the subjects, whereas only 83% of the dose was recovered from the third subject, probably due to his failure to collect all urine. DISCUSSION A number of high-performance liquid chromatographic assays have been developed for the analysis of aminoglycosides in serum (1,2,6,7,10). The present method is comparable in specificity and improved in accuracy.…”

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confidence: 99%

“…limits the applicability of these methods for routine clinical use (1, 2). Others have employed derivatization of aminoglycosides in serum and quantitation by measuring the peak height or peak area of the desired derivative (6,7,10 …”

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confidence: 99%

“…For limits the applicability of these methods for routine clinical use (1,2). Others have employed derivatization of aminoglycosides in serum and quantitation by measuring the peak height or peak area of the desired derivative (6,7,10 ) and a guard column packed with CO: Pell ODS (Whatman, Inc., Clifton, N.J.) were also used. The flow rate was 1.6 ml/min at ambient temperature.…”

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confidence: 99%

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High-pressure liquid chromatography analysis and single-dose disposition of tobramycin in human volunteers

Haughey¹,

Janicke²,

Adelman³

et al. 1980

Antimicrob Agents Chemother

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A sensitive and specific fluorometric high-pressure liquid chromatography technique was developed to measure both tobramycin and an internal standard (gentamicin C2). The assay utilizes direct extraction of the o-phthalaldehyde derivatives from serum and urine. Coefficients of variation were 7.9% (serum) and 6.0% (urine) at a tobramycin concentration of 1.0 ,tg/ml. The lower limit of assay sensitivity was 0.2 ,ug/ml. Results obtained from high-pressure liquid chromatography were in excellent agreement with those from radioimmunoassay for both serum (r = 0.97) and urine (r = 0.91). No other aminoglycoside antibiotics and no other antibiotics that were tested caused interfering peaks. Tobramycin (1 mg/kg intravenous bolus) was administered to three healthy volunteers. Tobramycin concentrations were detectable for 10 h in serum and for 240 h in urine after a 1-mg/kg intravenous dose. A two-compartment pharmacokinetic model was required to describe the tobramycin disposition. Urinary recovery of tobramycin over a 10-day period accounted for 95.8, 94.3, and 83.1% of the administered dose. High-pressure liquid chromatography methodology is sufficiently sensitive to determine single-dose, two-compartment tobramycin pharmacokinetics from urinary excretion data, thus verifying the prolonged excretion of tobramycin after a single dose. The analytical methodology and pharmacokinetic techniques described may be useful in studying other aminoglycosides. limits the applicability of these methods for routine clinical use (1, 2). Others have employed derivatization of aminoglycosides in serum and quantitation by measuring the peak height or peak area of the desired derivative (6,7,10

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confidence: 99%

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confidence: 99%

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confidence: 99%

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High-pressure liquid chromatography analysis and single-dose disposition of tobramycin in human volunteers

Haughey¹,

Janicke²,

Adelman³

et al. 1980

Antimicrob Agents Chemother

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“…A detailed literature review indicated that there are few analytical and bioanalytical methods were reported like calorimetry [4], HPLC [5][6][7][8], LCMS [9] and immunoassay [9]. But till date, there were no reported methods for UV visible Spectroscopy by using choloranilic acid as a derivatizing agent.…”

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confidence: 99%

Spectrophotometric Method for the Determination Amikacin in Pure and Pharmaceutical Dosage Form

Teja

Gurupadayya

Sairam

2018

Int J Curr Pharm Sci

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Objective: The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation using the spectrophotometric method.Methods: Due to lack of chromophoric group in the amikacin, it was derivatized with 0.1 mmol chloranillic acid reagent. For the estimation of amikacin, Shimadzu UV-1700 model spectrophotometer with UV probe software was used. The method was based on simple charge transfer complexation of the drug with a p-chloranillic acid reagent to give a purple coloured product which was measured at 524nm against blank solution. Results:The derivatised product of amikacin was detected at a wavelength of 524 nm. Linearity was observed with the concentration range of 20-100 µg/ml with a regression coefficient of 0.9803. Results of all the parameters were within the acceptance criteria with % RSD less than 2. Conclusion:The spectroscopic method was validated as per ICH guidelines and was found to be applicable for routine quantitative analysis of amikacin in marketed formulations also. The results of linearity, precision, accuracy LOD and LOQ were within the specified limits. The method is highly sensitive, robust, reproducible and specific.

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Aminoglycoside antibiotics — two decades of their hplc bioanalysis

Šoltés

1999

Biomed. Chromatogr.

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Amikacin Assay in Serum by High-Performance Liquid Chromatography

Abstract: A high-performance liquid chromatographic method for the quantitative determination of amikacin in serum is described. The

Cited by 36 publications

References 13 publications

High-pressure liquid chromatography analysis and single-dose disposition of tobramycin in human volunteers

High-pressure liquid chromatography analysis and single-dose disposition of tobramycin in human volunteers

Spectrophotometric Method for the Determination Amikacin in Pure and Pharmaceutical Dosage Form

Aminoglycoside antibiotics — two decades of their hplc bioanalysis

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