Amine-functionalized graphene as an effective electrochemical platform toward easily miRNA hybridization detection

Salimi, Abdollah; Kavosi, Begard; Navaee, Aso

doi:10.1016/j.measurement.2019.05.008

Cited by 28 publications

(8 citation statements)

References 56 publications

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“…Note that concentration of miR-155 in primary and metastatic breast cancer were reported to be 2 and 6 pM, respectively. [29] This suggests that our HCR-AgNCs nanobiosensor has great potential for real application in complex biological samples.…”

Section: Resultsmentioning

confidence: 88%

Ratiometric Detection of microRNA Using Hybridization Chain Reaction and Fluorogenic Silver Nanoclusters

Wong

New

2021

Chemistry An Asian Journal

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miRNA (miR)-155 is a potential biomarker for breast cancers. We aimed at developing a nanosensor for miR-155 detection by integrating hybridization chain reaction (HCR) and silver nanoclusters (AgNCs). HCR serves as an enzymefree and isothermal amplification method, whereas AgNCs provide a built-in fluorogenic detection probe that could simplify the downstream analysis. The two components were integrated by adding a nucleation sequence of AgNCs to the hairpin of HCR. The working principle was based on the influence of microenvironment towards the hosted AgNCs, whereby unfolding of hairpin upon HCR has manipulated the distance between the hosted AgNCs and cytosine-rich toehold region of hairpin. As such, the dominant emission of AgNCs changed from red to yellow in the absence and presence of miR-155, enabling a ratiometric measurement of miR with high sensitivity. The limit of detection (LOD) of our HCR-AgNCs nanosensor is 1.13 fM in buffered solution. We have also tested the assay in diluted serum samples, with comparable LOD of 1.58 fM obtained. This shows the great promise of our HCR-AgNCs nanosensor for clinical application.

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Section: Resultsmentioning

confidence: 88%

Ratiometric Detection of microRNA Using Hybridization Chain Reaction and Fluorogenic Silver Nanoclusters

Wong

New

2021

Chemistry An Asian Journal

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“…Otherwise, DPV based on ferriferrocyanide was also employed for miRNA detection [ 172 , 173 , 174 ]. In this case, advanced surface modification was performed to obtain an efficient electron transfer.…”

Section: Electrochemical Label-free Biosensingmentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

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“…As an example, Abdollah Salimi et al. proposed an amino graphene (AGr)‐based sensing platform for the detection of miRNA‐155, and analyzed its complementary target miRNA‐155 hybridization using DPV [65] . The as‐prepared biosensors were able to detect miRNA‐155 at nanogram concentration levels.…”

Section: Carbon Nanomaterials For Microrna Electrochemical Biosensingmentioning

confidence: 99%

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Wang

Wen

Yan

2020

Chemistry An Asian Journal

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MicroRNA (miRNA) is an important tumor marker in the human body, and its early detection has a great influence on the survival rate of patients. Although there are many detection methods for miRNA at present such as northern blotting, real‐time quantitative polymerase chain reaction, microarrays, and others, electrochemical biosensors have the advantages of low detection cost, small instrument size, simple operation, non‐invasive detection and low consumption of reagents and solvents, and thus they play an important role in the early detection of cancer. In addition, with the development of nanotechnology, nano‐biosensors show great potential. The application of various nanomaterials in the development of electrochemical biosensor has greatly improved the detection sensitivity of electrochemical biosensor. Among them, carbon nanomaterials which have unique electrical, optical, physical and chemical properties have attracted increasing attention. In particular, they have a large surface area, good biocompatibility and conductivity. Therefore, carbon nanomaterials combined with electrochemical methods can be used to detect miRNA quickly, easily and sensitively. In this review, we systematically review recent applications of different carbon nanomaterials (carbon nanotubes, graphene and its derivatives, graphitic carbon nitride, carbon dots, graphene quantum dots and other carbon nanomaterials) for miRNA electrochemical detection. In addition, we demonstrate the future prospects of electrochemical biosensors modified by carbon nanomaterials for the detection of miRNAs, and some suggestions for their development in the near future.

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Amine-functionalized graphene as an effective electrochemical platform toward easily miRNA hybridization detection

Cited by 28 publications

References 56 publications

Ratiometric Detection of microRNA Using Hybridization Chain Reaction and Fluorogenic Silver Nanoclusters

Ratiometric Detection of microRNA Using Hybridization Chain Reaction and Fluorogenic Silver Nanoclusters

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

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