1963
DOI: 10.1016/s0031-9422(00)82966-0
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Amino acid-activating enzymes in germinating pea seedlings

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1967
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Cited by 33 publications
(17 citation statements)
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“…However, the supply of ribosomes in the etioplasts is probably not the only factor limiting the synthesis of plastid proteins and irradiation with red light may also accelerate synthesis of plastid-specific mRNA, tRNA and aminoacyl tRNA synthetase. Total synthetase activity does increase in etiolated pea seedlings following irradiation with red light, but, as in the case of total RNA, the increase is proportional to the increase in tissue weight (6). It is not clear whether the effect of irradiation with red light is to change the course, as well as to increase the pace of the development of the dark-grown pea.…”
Section: Discussionmentioning
confidence: 96%
“…However, the supply of ribosomes in the etioplasts is probably not the only factor limiting the synthesis of plastid proteins and irradiation with red light may also accelerate synthesis of plastid-specific mRNA, tRNA and aminoacyl tRNA synthetase. Total synthetase activity does increase in etiolated pea seedlings following irradiation with red light, but, as in the case of total RNA, the increase is proportional to the increase in tissue weight (6). It is not clear whether the effect of irradiation with red light is to change the course, as well as to increase the pace of the development of the dark-grown pea.…”
Section: Discussionmentioning
confidence: 96%
“…Henshall & Goodwin (1964) indicated that the activity of the aminoacyl-tRNA synthetase was a limiting factor, and the involvement of tRNA species in the control of protein synthesis has been studied in detail (Stent, 1964; Anderson, 1969;Sueoka & KanoSueoka, 1970). It is therefore apparent that an understanding of the conditions affecting aminoacyl-tRNA formation is essential to cell-free investigations on the mechanism of protein synthesis.…”
mentioning
confidence: 99%
“…The increase in optical density of the stupernatan,t fraction from the beginning to the en4 of the 3-houir incubatioin was in,dicative of proteolytic acti,vity. A similar assay has been uised by several investigators (2,5,9). The amouint of free a-anvIino groutps in the superiiataiit soluitioni wxas determined by ninhydrin assay in vvhich absorption at 570 mpt was measuired (8) There was an increase in the proteolytic activity in cotyledons of in-tact embryos during the first 3 days of germination (table III).…”
Section: Methodsmentioning
confidence: 93%
“…2 Present address: Department of Crop Science, Michigan State University. activity between the axis and cotyledons.…”
mentioning
confidence: 99%