Amino acid and lactate catabolism in trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735

Ringø, Einar; Stenberg, Even; Strøm, Arne R.

doi:10.1128/aem.47.5.1084-1089.1984

Cited by 58 publications

(27 citation statements)

References 25 publications

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“…Bacterial culture medium and growth conditions. To determine the Na+ requirement of the organism, medium C, de-scribed previously (20), was used with the following modifications. NaCl (0.1 M) was included or omitted as indicated below.…”

Section: Methodsmentioning

confidence: 99%

Trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735: Na+-stimulated anaerobic transport in cells and membrane vesicles

Stenberg¹,

Ringø²,

Strøm³

1984

Appl Environ Microbiol

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Alteromonas putrefaciens NCMB 1735 required the presence of NaCl for anaerobic growth with serine, cysteine, and formate as substrate and trimethylamine oxide (TMAO) as external electron acceptor. When lactate was substrate, the organism grew equally well in the absence of NaCl. Anaerobic uptake of glutamate, aspartate, serine, cysteine, and lactate in resting cells was strongly stimulated with NaCl, and cytoplasmic membrane vesicles energized by electron transfer from formate to TMAO displayed active Na+-dependent uptake of serine. The data suggested that participation in transport processes was the only vital function of Na+ in A. putrefaciens. Formateand TMAO-dependent anaerobic serine uptake in vesicles was sensitive to the protonophore carbonyl cyanide m-chlorophenyl-hydrazone and the ionophores valinomycin and gramicidin. Transport-active vesicles contained cytochromes of b and c type, and both serine uptake and TMAO reduction with formate were inhibited with the electron transfer inhibitor 2-heptyl-4-hydroxyquinoline N-oxide. Thus, reduction of TMAO to trimethylamine in A. putrefaciens appeared to be coupled with a chemiosmotic mechanism of energy conversion. Most gram-negative heterotrophic marine bacteria require NaCl for growth (19). The demand for NaCl correlates with a specific requirement for Na+ in transport processes of the marine bacterium Alteromonas haloplanktis (17, 25, 26). Unlike other gram-negative bacteria from marine sources, many isolates of Alteromonas putrefaciens from fisheries are able to grow in media without NaCl (12, 13). When we examined trimethylamine oxide (TMAO)-dependent anaerobic growth of A. putrefaciens NCMB 1735 with various amino acids and simple metabolites as substrate (20), we found that the demand for NaCl for growth varied with the substrate. This initiated a further investigation into the requirement for Na+ for anaerobic growth and for transport processes. The previous growth data for A. putrefaciens indicate that TMAO acts as a terminal electron acceptor in an anaerobic respiration coupled with energy conservation (20), similar to that found in enteric bacteria (27, 28). In bacteria, the proton motive force generated by electron transfer in membranebound respiratory systems promotes active transport of amino acids and certain other metabolites (7). The transport can be driven directly by the components of the proton motive force, i.e., the transmembrane pH gradient and the membrane potential, or more indirectly by an Na+ gradient set up by an Na+-H+ antiport system (1, 16, 23, 29). Studies of transport processes in cytoplasmic membrane vesicles are useful to examine both energy conservation in different types of anaerobic respiration in fermentative bacteria (7, 27) and the role of Na+ in transport processes of various bacteria such as A. haloplanktis (25, 26), Halobacterium halobium (11), Pseudomonas aeruginosa (4), and Escherichia coli (3, 14). Therefore, we have prepared transport-active vesicles of A. putrefaciens and studied the properties. We

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Section: Methodsmentioning

confidence: 99%

Trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735: Na+-stimulated anaerobic transport in cells and membrane vesicles

Stenberg¹,

Ringø²,

Strøm³

1984

Appl Environ Microbiol

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“…Few studies have explicitly examined individual amino acids as potential electron donors for anaerobic respiration. Serine, cysteine, glutamate, and aspartate are effective electron donors for anaerobic respiration by Alteromonas (Shewanllla) putrefaciens NCMB 1735 using trimethylamine oxide as the terminal electron acceptor [80]. Geovibrio ferrireducens can grow by coupling the oxidation of proline to the reduction of Fe(III) [6].…”

Section: Electron Donor Utilization and Dissimilatory Iron(iii) Reducmentioning

confidence: 99%

Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32

et al. 2019

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The bioreduction of Fe(III) oxides by dissimilatory iron reducing bacteria (DIRB) may result in the production of a suite of Fe(II)-bearing secondary minerals, including magnetite, siderite, vivianite, green rusts, and chukanovite; the formation of specific phases controlled by the interaction of various physiological and geochemical factors. In an effort to better understand the effects of individual electron donors on the formation of specific Fe(II)-bearing secondary minerals, we examined the effects of a series of potential electron donors on the bioreduction of lepidocrocite (γ-FeOOH) by Shewanella putrefaciens CN32. Biomineralization products were identified by X-ray diffraction, Mössbauer spectroscopy, and scanning electron microscopy. Acetate, citrate, ethanol, glucose, glutamate, glycerol, malate, and succinate were not effectively utilized for the bioreduction of lepidocrocite by S. putrefaciens CN32; however, substantial Fe(II) production was observed when formate, lactate, H2, pyruvate, serine, or N acetylglucosamine (NAG) was provided as an electron donor. Carbonate or sulfate green rust was the dominant Fe(II)-bearing secondary mineral when formate, H2, lactate, or NAG was provided, however, siderite formed with pyruvate or serine. Geochemical modeling indicated that pH and carbonate concentration are the key factors determining the prevalence of carbonate green rust verses siderite.

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“…TMA is typically present during fish spoilage. Stenberg et al (1984) and Ringo et al (1984) have also demonstrated the importance of the culture conditions and of the composition of the medium (NaC1, serine, L-cysteine, formate, lactate, glutamate, aspartate) for the development of Shewanella putrefaciens.…”

mentioning

confidence: 99%

DETECTION AND ENUMERATION OF H₂S⁺ BACTERIA: APPLICATION TO SHEWANELLA PUTREFACIENS (CIP)

Malle¹,

Vallé²,

Demarque³

et al. 1998

Rapid Methods Automation Mic

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H2S+ bacteria responsible for the degradation of sulfur‐containing amino acids of fish muscle are currently little used to evaluate the microbiological pal quality of fish. Shewanella putrefaciens greatly predominates in this flora, and was therefore used to define a suitable culture method and medium. Inoculations by the Spiral surface method at 25C, with an incubation of 72h, gave the best counts on a medium containing two sources of sulfur (organic and inorganic) for H2S+ bacteria. The culture medium and the NaCl concentration were determinant in the evaluation of this flora. At present there is no standard medium which meets these requirements.

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Amino acid and lactate catabolism in trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735

Cited by 58 publications

References 25 publications

Trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735: Na+-stimulated anaerobic transport in cells and membrane vesicles

Trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735: Na+-stimulated anaerobic transport in cells and membrane vesicles

Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32

DETECTION AND ENUMERATION OF H₂S⁺ BACTERIA: APPLICATION TO SHEWANELLA PUTREFACIENS (CIP)

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Amino acid and lactate catabolism in trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735

Cited by 58 publications

References 25 publications

Trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735: Na+-stimulated anaerobic transport in cells and membrane vesicles

Trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735: Na+-stimulated anaerobic transport in cells and membrane vesicles

Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32

DETECTION AND ENUMERATION OF H2S+ BACTERIA: APPLICATION TO SHEWANELLA PUTREFACIENS (CIP)

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DETECTION AND ENUMERATION OF H₂S⁺ BACTERIA: APPLICATION TO SHEWANELLA PUTREFACIENS (CIP)