Amino acid-balanced diets improved DSS-induced colitis by alleviating inflammation and regulating gut microbiota

Li, Sen; Huang, Kai; Liang, Ting; Chen, Yu; Guan, Yingjie; Shang, Ruizhi; Guan, Tao; Wu, Jihuai; Chen, Yingwei; Guan, Xiaohong

doi:10.1007/s00394-022-02906-y

Cited by 12 publications

(6 citation statements)

References 40 publications

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“…Clinical studies indicated that the richness and diversity of intestinal microbiota were significantly reduced in patients with colitis . At the same time, previous evidence suggested that the increase in intestinal flora richness was accompanied by a decrease in pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β, indicating that regulating the intestinal flora is extremely helpful for curing colitis . Interestingly, NEPs could significantly promote the proliferation of Dubosiella and Enterorhabdus and remarkably inhibit the proliferation of Alistipes and Romboutsia in UC mice.…”

Section: Discussionmentioning

confidence: 93%

“…39 At the same time, previous evidence suggested that the increase in intestinal flora richness was accompanied by a decrease in pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β, indicating that regulating the intestinal flora is extremely helpful for curing colitis. 40 Interestingly, NEPs could significantly promote the proliferation of Dubosiella and Enterorhabdus and remarkably inhibit the proliferation of Alistipes and Romboutsia in UC mice. Among them, Enterorhabdus could decompose polysaccharides to produce organic acids and obtain the energy mainly in this way, 41 and Dubosiella has also been proved to be related to the production of SCFAs.…”

Section: Discussionmentioning

confidence: 98%

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Nonextractable Polyphenols from Fu Brick Tea Alleviates Ulcerative Colitis by Controlling Colon Microbiota-Targeted Release to Inhibit Intestinal Inflammation in Mice

Yang,

Wang

et al. 2024

J. Agric. Food Chem.

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This study was designed to elucidate the colon microbiota-targeted release of nonextractable bound polyphenols (NEPs) derived from Fu brick tea and to further identify the possible anti-inflammatory mechanism in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice. 1.5% DSS drinking water-induced C57BL/6J mice were fed rodent chow supplemented with or without 8% NEPs or dietary fibers (DFs) for 37 days. The bound p-hydroxybenzoic acid and quercetin in NEPs were liberated up to 590.5 ± 70.6 and 470.5 ± 51.6 mg/g by in vitro human gut microbiota-simulated fermentation, and released into the colon of the mice supplemented with NEPs by 4.4-and 1.5-fold higher than that of the mice supplemented without NEPs, respectively (p < 0.05). Supplementation with NEPs also enhanced the colonic microbiota-dependent production of SCFAs in vitro and in vivo (p < 0.05). Interestingly, Ingestion of NEPs in DSS-induced mice altered the gut microbiota composition, reflected by a dramatic increase in the relative abundance of Dubosiella and Enterorhabdus and a decrease in the relative abundance of Alistipes and Romboutsia (p < 0.05). Consumption of NEPs was demonstrated to be more effective in alleviating colonic inflammation and UC symptoms than DFs alone in DSS-treated mice (p < 0.05), in which the protective effects of NEPs against UC were highly correlated with the reconstruction of the gut microbiome, formation of SCFAs, and release of bound polyphenols. These findings suggest that NEPs as macromolecular carriers exhibit targeted delivery of bound polyphenols into the mouse colon to regulate gut microbiota and alleviate inflammation.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Nonextractable Polyphenols from Fu Brick Tea Alleviates Ulcerative Colitis by Controlling Colon Microbiota-Targeted Release to Inhibit Intestinal Inflammation in Mice

Yang,

Wang

et al. 2024

J. Agric. Food Chem.

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“…Proteobacteria were found to be significantly less abundant, which is consistent with earlier research that linked high proteobacteria levels to IBD. Firmicutes and Bacteroidetes , prominent components of the intestinal microbiota, are known for their protective effects against IBD [ 58 , 59 ]. The relative abundance of Bacteroidetes and Firmicutes was partially regulated by PIMP treatment, which further supported the significance of their balance.…”

Section: Discussionmentioning

confidence: 99%

Porcine Intestinal Mucosal Peptides Target Macrophage-Modulated Inflammation and Alleviate Intestinal Homeostasis in Dextrose Sodium Sulfate-Induced Colitis in Mice

Wang,

Xie,

et al. 2024

Foods

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Porcine intestinal mucosal proteins are novel animal proteins that contain large amounts of free amino acids and peptides. Although porcine intestinal mucosal proteins are widely used in animal nutrition, the peptide bioactivities of their enzymatic products are not yet fully understood. In the present study, we investigated the effect of porcine intestinal mucosal peptides (PIMP) on the RAW264.7 cell model of LPS-induced inflammation. The mRNA expression of inflammatory factors (interleukin 6, tumor necrosis factor-α, and interleukin-1β) and nitrous oxide levels were all measured by quantitative real-time PCR and cyclooxygenase-2 protein expression measured by Western blot. To investigate the modulating effect of PIMP and to establish a model of dextran sodium sulfate (DSS)-induced colitis in mice, we examined the effects of hematoxylin-eosin staining, myeloperoxidase levels, pro-inflammatory factor mRNA content, tight junction protein expression, and changes in intestinal flora. Nuclear factor κB pathway protein levels were also assessed by Western blot. PIMP has been shown in vitro to control inflammatory responses and prevent the activation of key associated signaling pathways. PIMP at doses of 100 and 400 mg/kg/day also alleviated intestinal inflammatory responses, reduced tissue damage caused by DSS, and improved intestinal barrier function. In addition, PIMP at 400 mg/kg/day successfully repaired the dysregulated gut microbiota and increased short-chain fatty acid levels. These findings suggest that PIMP may positively influence inflammatory responses and alleviate colitis. This study is the first to demonstrate the potential of PIMP as a functional food for the prevention and treatment of colitis.

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“…Studies using a dextran sulfate sodium-induced colitis mouse model showed that Parasutterella was a harmful bacterium and that diet modification (Li et al, 2022) or crocetin (Dang et al, 2022) could decrease the abundance of Parasutterella. Maternal intake of inulin can aggravate intestinal damage and inflammation in the offspring of rats with colitis through regulating the intestinal microbiota, Frontiers in Pharmacology frontiersin.org including increasing the abundance of Parasutterella.…”

Section: Discussionmentioning

confidence: 99%

Cannabis sativa L. alleviates loperamide-induced constipation by modulating the composition of gut microbiota in mice

et al. 2022

Front. Pharmacol.

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MaZiRenWan (MZRW) is the most frequently used Traditional Chinese Medicine formula to treat chronic constipation, Cannabis sativa L. is regarded as a monarch drug in MZRW. However, the targets of Cannabis sativa L. that enhance colonic motility and improve constipation symptoms remain unknown. This study was designed to investigate the laxative effect and underlying mechanism of the water extract of Cannabis sativa L. (WECSL) using a loperamide-induced constipation mouse model. We found that WECSL treatment significantly improved intestinal motility and water-electrolyte metabolism, decreased inflammatory responses, prevented gut barrier damage, and relieved anxiety and depression in constipated mice. WECSL also structurally remodeled the composition of the gut microbiota and altered the abundance of bacteria related to inflammation, specifically Butyricicoccus and Parasutterella. Moreover, WECSL failed to relieve constipation symptoms following intestinal flora depletion, indicating that WECSL alleviates constipation symptoms depending on the gut microbiota. Our research provides a basis for WECSL to be further investigated in the treatment of constipation from the perspective of modern medicine.

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Amino acid-balanced diets improved DSS-induced colitis by alleviating inflammation and regulating gut microbiota

Cited by 12 publications

References 40 publications

Nonextractable Polyphenols from Fu Brick Tea Alleviates Ulcerative Colitis by Controlling Colon Microbiota-Targeted Release to Inhibit Intestinal Inflammation in Mice

Nonextractable Polyphenols from Fu Brick Tea Alleviates Ulcerative Colitis by Controlling Colon Microbiota-Targeted Release to Inhibit Intestinal Inflammation in Mice

Porcine Intestinal Mucosal Peptides Target Macrophage-Modulated Inflammation and Alleviate Intestinal Homeostasis in Dextrose Sodium Sulfate-Induced Colitis in Mice

Cannabis sativa L. alleviates loperamide-induced constipation by modulating the composition of gut microbiota in mice

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