Amino Acid Decarboxylase Enzymes – Vital or Irrelevant to Gastric Secretion?

Histamine is involved in many important biological processes such as allergic reactions, gastric acid secretion and neurotransmission. The formation of histamine is catalysed by the enzyme histidine decarboxylase. In order to understand the role of histamine in different tissues, information about the cellular localisation of the decarboxylase is important. However, the availability of antisera against the enzyme, which can be used in immunocytochemical techniques, has so far been limited, mainly due to the difficulties in purifying sufficient amounts of histidine decarboxylase from various tissues. In the present study we describe the use of antisera raised against rat recombinant histidine decarboxylase to localise the enzyme immunocytochemically in the gastric mucosa of different mammals and submammalian vertebrates. The antisera specifically stained histidine decarboxylase-immunoreactive cells in the gastric mucosa of not only rat, but also of species like frog, chicken, mouse and dog. This is the first report describing the immunocytochemical distribution of the decarboxylase in the gastric mucosa of species other than rat. These antisera are likely to become valuable tools for further studies of the immunocytochemical localisation of histidine decarboxylase.

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Investigation of the Role of Extracellular Calcium in the Control of Acid Secretion in the Isolated Whole Stomach of the Rat

BUNCE,

HONEY,

PARSONS

1979

British J Pharmacology

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An isolated stomach preparation from immature rats has been used to study the role of extracellular calcium in the control of gastric acid secretion. Calcium was removed from both the serosal and mucosal solutions either in the absence of a chelating agent or in the presence of EGTA. Removal of calcium in the absence of EGTA had no significant effect on basal acid secretion or the acid responses to gastrin and dibutyryl cyclic adenosine 3′,5′‐monophosphate (db cyclic AMP). Under the same conditions there was a marked potentiation of the acid response to histamine, and a reduction of the acid response to acetylcholine which was readily reversed on restoring calcium to the bathing solutions. Removal of calcium in the presence of EGTA caused an inhibition of basal acid secretion and of the acid responses to histamine and db cyclic AMP. In each case this reduction in acid output was readily reversed on bathing the stomachs in normal calcium‐containing (2.5 mm Ca2+) EGTA‐free solutions. The inhibition of the acid response to histamine produced by Ca2+ ‐free solutions which contained EGTA was not reversed on bathing the stomachs in solutions that contained both EGTA (0.1 mm) and an excess of calcium (2.5 mm). The removal of extracellular calcium in the absence of EGTA provided evidence that the secretion of H+ ions is dependent under some conditions on calcium ions. The possibility cannot be excluded that EGTA itself exerts an inhibitory influence on the process of acid secretion.

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Amino Acid Decarboxylase Enzymes – Vital or Irrelevant to Gastric Secretion?

Cited by 3 publications

References 75 publications

Purification et detection de plusieurs formes moleculaires de l'histidine decarboxylase de la muqueuse gastrique de rat

Purification et detection de plusieurs formes moleculaires de l'histidine decarboxylase de la muqueuse gastrique de rat

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Investigation of the Role of Extracellular Calcium in the Control of Acid Secretion in the Isolated Whole Stomach of the Rat

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