Amino Acid Production from Rice Straw Hydrolyzates

Matano, Christian; Meiswinkel, Tobias M.; Wendisch, Volker F.

doi:10.1016/b978-0-12-401716-0.00038-6

Cited by 8 publications

(3 citation statements)

References 151 publications

(123 reference statements)

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“…5 a). This indicates that acetate was activated to acetyl-CoA and completely metabolized from the fermentation broth via the TCA cycle to carbon dioxide, which agrees with findings of Matano et al [ 47 ] and Gebhardt et al [ 48 ].…”

Section: Resultssupporting

confidence: 91%

Tolerance and metabolic response of Pseudomonas taiwanensis VLB120 towards biomass hydrolysate-derived inhibitors

Wordofa

Kristensen

2018

Biotechnol Biofuels

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BackgroundBio-conversion of lignocellulosic biomass to high-value products offers numerous benefits; however, its development is hampered by chemical inhibitors generated during the pretreatment process. A better understanding of how microbes naturally respond to those inhibitors is valuable in the process of designing microorganisms with improved tolerance. Pseudomonas taiwanensis VLB120 is a natively tolerant strain that utilizes a wide range of carbon sources including pentose and hexose sugars. To this end, we investigated the tolerance and metabolic response of P. taiwanensis VLB120 towards biomass hydrolysate-derived inhibitors including organic acids (acetic acid, formic acid, and levulinic acid), furans (furfural, 5-hydroxymethylfurfural), and phenols (vanillin).ResultsThe inhibitory effect of the tested compounds varied with respect to lag phase, specific growth rate, and biomass yield compared to the control cultures grown under the same conditions without addition of inhibitors. However, P. taiwanensis was able to oxidize vanillin and furfural to vanillic acid and 2-furoic acid, respectively. Vanillic acid was further metabolized, whereas 2-furoic acid was secreted outside the cells and remained in the fermentation broth without further conversion. Acetic acid and formic acid were completely consumed from the fermentation broth, while concentration of levulinic acid remained constant throughout the fermentation process. Analysis of free intracellular metabolites revealed varying levels when P. taiwanensis VLB120 was exposed to inhibitory compounds. This resulted in increased levels of ATP to export inhibitors from the cell and NADPH/NADP ratio that provides reducing power to deal with the oxidative stress caused by the inhibitors. Thus, adequate supply of these metabolites is essential for the survival and reproduction of P. taiwanensis in the presence of biomass-derived inhibitors.ConclusionsIn this study, the tolerance and metabolic response of P. taiwanensis VLB120 to biomass hydrolysate-derived inhibitors was investigated. P. taiwanensis VLB120 showed high tolerance towards biomass hydrolysate-derived inhibitors compared to most wild-type microbes reported in the literature. It adopts different resistance mechanisms, including detoxification, efflux, and repair, which require additional energy and resources. Thus, targeting redox and energy metabolism in strain engineering may be a successful strategy to overcome inhibition during biomass hydrolysate conversion and lead to development of more robust strains.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1192-y) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 91%

Tolerance and metabolic response of Pseudomonas taiwanensis VLB120 towards biomass hydrolysate-derived inhibitors

Wordofa

Kristensen

2018

Biotechnol Biofuels

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“…It was realized that the protein content of unstabilized rice bran was 13.0%, whereas the highest protein content (13.6%) was observed for the rice bran sample exposed to longer heat treatment (Patil et al 2016). With the increase in the time of exposure at any given available power, protein content was increased (Malik and Saini 2017;Wang et al 2017a) Despite all of the functional potential of rice bran, there have been relatively few attempts to use this material in bacterial culture and basically no use in Acetobacter xylinum species synthesis (Zhu et al 2013;Matano et al 2014). Therefore, this research focuses on the use of rice bran as an alternative nitrogen source to yeast extract in Acetobacter xylinum bacterial cellulose synthesis.…”

Section: Introductionmentioning

confidence: 99%

Rice Bran, an Alternative Nitrogen Source for Acetobacter xylinum Bacterial Cellulose Synthesis

et al. 2018

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Rice bran was introduced as an alternative nitrogen source to Hestrin and Schramm (HS) medium in HS Acetobacter xylinum culture media in bacterial cellulose (B.C) synthesis. The results indicated an unchanged composition of crude protein per increase in incubation days while diluted protein increased with incubation period. Fourier transform infrared spectroscopy (FTIR) results showed the absorbance of OH groups. It was confirmed that the BC yield was directly proportional to the rice bran content. A general observation indicated a gradual transformation from a highly crystalline structure to a very amorphous structure as rice bran content increased with the endothermic decomposition of samples being recorded between 113.5 °C and 125.6 °C.

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“…However, the production of petroleum-based SA posed problems of carbon footprint emissions and price volatility, which subsequently lead to the development of bio-based SA. Recent breakthroughs in the biofermentation technology make SA more abundant and a competitive supply. − However, while there has been an increase of interest in various renewable chemicals such as glucose, , fructose, , and levulinic acid (LA), , only scant attention has been paid for the catalytic conversion of SA despite that a large number of value-added products can be converted from it; that is, γ-butyrolactone (GBL), 1,4-butanediol (BDO), tetrahydrofuran (THF), N -methyl-2-pyrrolidone, and several other derivatives. , Among these potential products, the conversion of bio-derived SA to BDO has been recognized as one of the most important industrial processes. Particularly, BDO can be utilized as a starting material for various commodity compounds and combined with SA to prepare polybutylene succinate and poly(butylene succinate- co -butylene terephthalate), which are known as biodegradable plastics …”

Section: Introductionmentioning

confidence: 99%

Highly Selective Synthesis of 1,4-Butanediol via Hydrogenation of Succinic Acid with Supported Cu–Pd Alloy Nanoparticles

Nishimura

2019

ACS Sustainable Chem. Eng.

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Hydroxyapatite (HAP)-supported Cu−Pd catalysts, denoted as Cu−Pd/HAP, have been discovered to be the efficient catalysts for hydrogenation of bioderived succinic acid in which the products are selectively tunable by adjusting the mixing ratio of Cu and Pd. Optimal performance toward 1,4-butanediol (BDO) is observed with Cu−Pd/HAP prepared with 8 wt %-Cu and 2 wt %-Pd, affording a selectivity of 82% at quantitative conversion. In contrast, the monometallic Cu and Pd catalysts are unable to produce BDO as the major product, but only γ-butyrolactone (GBL) with a low yield of 16% and butyric acid (BA) with a considerable yield of 78%, respectively, is formed. The formation of well-dispersed bimetallic alloy nanoparticles is revealed by transmission electron microscopy with energy-dispersive spectroscopy, H 2 -temperature programmed reduction, X-ray diffraction, X-ray photoelectron spectroscopy, and X-ray absorption spectroscopy studies. It is concluded that the fine alloying structure with high Cu contents is significant in favoring the formation of BDO via the ring-opening step of GBL rather than the hydrogenation of GBL to the nontarget product of BA. In addition, the CuPd alloy catalyst exhibits good recycling ability in four consecutive runs without significant loss in its activity.

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Amino Acid Production from Rice Straw Hydrolyzates

Cited by 8 publications

References 151 publications

Tolerance and metabolic response of Pseudomonas taiwanensis VLB120 towards biomass hydrolysate-derived inhibitors

Tolerance and metabolic response of Pseudomonas taiwanensis VLB120 towards biomass hydrolysate-derived inhibitors

Rice Bran, an Alternative Nitrogen Source for Acetobacter xylinum Bacterial Cellulose Synthesis

Highly Selective Synthesis of 1,4-Butanediol via Hydrogenation of Succinic Acid with Supported Cu–Pd Alloy Nanoparticles

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