Amino Acid- vs. Peptide-Odorants: Responses of Individual Olfactory Receptor Neurons in an Aquatic Species

Hassenklöver, Thomas; Pallesen, Lars P.; Schild, Detlev; Manzini, Ivan

doi:10.1371/journal.pone.0053097

Cited by 11 publications

(15 citation statements)

References 57 publications

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“…Ten images before the onset of stimulus application were taken as control (F 0 ). In accordance with previous publications 2 3 13 16 23 a response was considered significant if three criteria were fulfilled: it had to occur within 10 s after stimulus application, the first two fluorescence values after stimulus arrival at the mucosa, F 1 and F 2 , had to be larger than the maximum of the F 0 values, and F 2 had to be larger than F 1 .…”

Section: Methodsmentioning

confidence: 68%

“…Odor responses were measured as changes of intracellular calcium concentrations of individual olfactory neurons in vibratome slices of the olfactory organs using Fluo-4/AM as calcium indicator dye, essentially as described previously 2 23 . A mix of thirteen amines (2-Phenylethylamine, tyramine, butylamine, cyclohexylamine, hexylamine, 3-methylbutylamine, N,N-dimethylethylamine, 2-methylbutylamine, 1-formylpiperidine, 2-methylpiperidine, N-ethylcyclohexylamine, 1-ethylpiperidine, piperidine) was used to analyse amine responses, ( cf.…”

Section: Methodsmentioning

confidence: 99%

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Different expression domains for two closely related amphibian TAARs generate a bimodal distribution similar to neuronal responses to amine odors

Syed

Sansone

Röner

et al. 2015

Sci Rep

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Olfactory perception is mediated by a multitude of olfactory receptors, whose expression in the sensory surface, the olfactory epithelium, is spatially regulated. A common theme is the segregation of different olfactory receptors in different expression domains, which in turn leads to corresponding segregation in the neuronal responses to different odor groups. The amphibian olfactory receptor gene family of trace amine associated receptors, in short TAARs, is exceedingly small and allows a comprehensive analysis of spatial expression patterns, as well as a comparison with neuronal responses to the expected ligands for this receptor family, amines. Here we report that TAAR4b exhibits a spatial expression pattern characteristically different in two dimensions from that of TAAR4a, its close homolog. Together, these two genes result in a bimodal distribution resembling that of amine responses as visualized by calcium imaging. A stringent quantitative analysis suggests the involvement of additional olfactory receptors in amphibian responses to amine odors.

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Section: Methodsmentioning

confidence: 68%

Section: Methodsmentioning

confidence: 99%

Different expression domains for two closely related amphibian TAARs generate a bimodal distribution similar to neuronal responses to amine odors

Syed

Sansone

Röner

et al. 2015

Sci Rep

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“…The olfactory system of Xenopus tadpoles shows an exquisite sensitivity to detect amino acids in water, which effectively behave as waterborne odorants (Hassenklöver, Pallesen, Schild, & Manzini, ). Through at least 36 classes of ORNs, Xenopus larvae elaborate a map of odors by activating specific glomeruli projecting to M/T cells (Manzini & Schild, ).…”

Section: Resultsmentioning

confidence: 99%

Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor‐guided behavior in Xenopus tadpoles

Terni

Pacciolla

Masanas

et al. 2017

J of Comparative Neurology

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Olfactory sensory neurons (OSNs) are chemoreceptors that establish excitatory synapses within glomeruli of the olfactory bulb. OSNs undergo continuous turnover throughout life, causing the constant replacement of their synaptic contacts. Using Xenopus tadpoles as an experimental system to investigate rewiring of glomerular connectivity, we show that novel OSN synapses can transfer information immediately after formation, mediating olfactory-guided behavior. Tadpoles recover the ability to detect amino acids 4 days after bilateral olfactory nerve transection. Restoration of olfactory-guided behavior depends on the efficient reinsertion of OSNs to the olfactory bulb. Presynaptic terminals of incipient synaptic contacts generate calcium transients in response to odors, triggering long lasting depolarization of olfactory glomeruli. The functionality of reconnected terminals relies on well-defined readily releasable and cytoplasmic vesicle pools. The continuous growth of non-compartmentalized axonal processes provides a vesicle reservoir to nascent release sites, which contrasts to the gradual development of cytoplasmic vesicle pools in conventional excitatory synapses. The immediate availability of fully functional synapses upon formation supports an age-independent contribution of OSNs to the generation of odor maps.

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“…Amino acid is a kind of compound that is highly effective chemosensory stimuli in fish. Behavioral evidence has shown that amino acids are detected by a variety of fish [3] and electrophysiological experiments have proven that amino acids are highly effective stimuli in both olfactory and gustatory systems [4]. Three types of olfactory related receptor have been reported in the olfactory epithelium of fish; they include; Olfactory receptors (OR), Vomeronasal Receptor (VR) and Trace-Amine Associated Receptors (TAAR) [5,6] OR genes are a type of G-protein coupled receptors and they are being expressed in the ciliated sensory neurons.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals L-amino Acids as Olfactory Stimulant in the Large Yellow Croaker (Larimichthys crocea)

Franklyn¹,

Wang²,

Yu³

et al. 2017

J Aquac Res Development

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Similar to bony fish, feeding preference in Larimichythys crocea is determined by the smell and taste of food which is mainly controlled by the olfactory system. Ten different L-types of amino acids at different concentration levels were introduced to the large yellow croaker and significant differences in the olfactory response with reference to odor detection were observed. Movement and feeding responds were taken within a 4-minutes interval which showed an increase in the responsiveness of the fish with increase in the concentration of specific amino acid from 0.01 M/L to 0.08 M/L. There was a significant difference between different kinds of amino acid with high responses from L-Arginine and L-Tryptophan. The olfactory epithelium before and after administering L-amino acid was observed and it showed remarkable differences with the top of the cilia being inflated in the administered amino acid samples. RNA sequenced was done with Illumina HiSeq 2500 in which 40326272 and 35794904 clean reads from two amino acids namely L-Arginine and L-Tryptophan were acquired respectively and more than 845% of the read were mapped to the reference genome. Comparing the gene expression of two L-types amino acid, 16701 unigenes were significantly differentially expressed including 10142 up-regulated and 6503 down-regulated genes. 100 olfactory receptor genes were detected, these genes include; Olfactory receptors (OR), Vomeronasal receptors (VR) and Trace-Amine Associated Receptors (TAAR) with low levels of Olfactomedin like Protien (OLFM) genes. OR expressed genes were then selected for further quantitative RT-PCR analysis. With the use of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the differential expressed genes were found out that multiple genes were involve in olfactory activities such as signaling, single organism signaling, cell communication and olfactory transduction. These transcriptome analyses provide clear information on olfactory related genes expression of Larimichthys crocea with respect to L-type amino acids. Also, this study help us identify which L-type amino acid is a potent phagostimulant to the fish and how it can be employed in the fish feed thereby increasing the protein intake of the fish.

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Amino Acid- vs. Peptide-Odorants: Responses of Individual Olfactory Receptor Neurons in an Aquatic Species

Cited by 11 publications

References 57 publications

Different expression domains for two closely related amphibian TAARs generate a bimodal distribution similar to neuronal responses to amine odors

Different expression domains for two closely related amphibian TAARs generate a bimodal distribution similar to neuronal responses to amine odors

Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor‐guided behavior in Xenopus tadpoles

Transcriptome Analysis Reveals L-amino Acids as Olfactory Stimulant in the Large Yellow Croaker (Larimichthys crocea)

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