Amino acids combined chitosan nanoparticles for controlled release of Doxorubicin hydrochloride

El‐Ghaffar, M. A. Abd; Akl, Magda Abdel-Aziz; Kamel, Amira Mostafa; Hashem, M.S.

doi:10.21608/ejchem.2017.745.1021

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…polyethylene glycol, polyacrylic acid, poly (vinyl pyrrolidone), poly (vinyl alcohol), poly (methacrylic acid) or natural, as in the case of chitosan, starch, cellulose, agarose, and dextran. Natural polymers, especially polysaccharide, are widely used in the field of drug delivery and biomedical applications [ 23 ]. Chitosan, the second most abundant biopolymer after cellulose, is an inexpensive, inert, hydrophilic, biocompatible support, and is thus attractive for drug delivery systems [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma

2023

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In this study, Superparamagnetic magnetite nanoparticles (SPMNPs) are used in a new way as direct nanocarrier for Doxorubicin hydrochloride (DOX) via the functionalization of their surface with tri-sodium citrate through ligand exchange to conjugate DOX with imine bond to form tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (DOX/Cit-MNPs). The DOX/Cit-MNPs were coated with chitosan to form chitosan coated citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) to offer biodegradability and pH-sensitive drug release features. The Fourier transform infrared spectroscopy (FTIR) analysis confirmed functionalization of SPMNPs, DOX-conjugation, and chitosan coating. The trans electron microscopy (TEM) show spherical nanostructures with average size 40 nm for coated nanocarriers. The saturation magnetization value of carrier was 59 emu/g.The in-vitro release of DOX from the chitosan coated tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) was studied to be 75% at pH 5.5 and 28.6% at pH 7.4 which proves the pH sensitivity of encapsulated Cs/DOX/Cit-MNPs. The effect of Cs/DOX/Cit-MNPs toward Human Breast Cancer Cell lines (MCF7) was studied and found to be 76% without magnet and 98% with external magnet after 72 h. With increasing DOX concentration and treatment time, the cell inhibition (IR%) of DOX solution and Cs/DOX-Cit-MNPs suspension to all cells is increased. Cs/DOX/Cit-MNPs showed sustained release and good inhibition to cancer cells and offer a protective mode for normal cells (WISH) compared to the free DOX. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma

2023

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“…Further, M. A. Abd El-Ghaffar loaded DOX in CsNPs and Cs-glutamate to improve EE. Their results demonstrated that as the DOX concentration was raised, the EE increased to reach the highest concentration of 69% for copolymer/DOX and 30% for CsNPs/DOX [32].…”

Section: Determination Of Drug Ee and DLmentioning

confidence: 99%

Redox-Sensitive Targeted Doxorubicin-Loaded Chitosan-based Nanoparticles to Treat Breast Cancer

2023

JPTCP

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The positive charge of chitosan (Cs) polymer has limited its utilization as a carrier for doxorubicin (DOX). Herein, a Cs derived by covalent linkage to L-Cysteine (L-Cys) was offered to reduce the positive charge of the polymer and to enhance the DOX entrapment efficiency (EE). Hence, the hyaluronic acid-targeted DOX-loaded Cs-Cys nanoparticles (HA/Cs-CysNPs-DOX) were synthesized as a redox-responsive carrier for intracellular delivery of DOX. At first, the Cs-CysNPs-DOX was synthesized by ion-gelation technique, after which HA was attached by electrostatic interaction as a targeting agent. Ultimately, they were characterized in terms of size, zeta potential, EE, drug loading (DL), and morphology. HA/Cs-CysNPs-DOX based on the novel copolymers displayed an appropriate EE for DOX and redox-stimuli drug release. The hemolysis assay proved the safety and hemocompatibility of the NPs, which confirms their intravenous application. In vitro drug release indicated high stability in physiological conditions with a glutathione (GSH) dependence drug release. The cytotoxicity and apoptosis of HA/Cs-CysNPs-DOX were also studied by investigating MCF-7 cells with various concentrations and times. A considerable cytotoxicity enhancement was displayed for HA/Cs-CysNPs-DOX compared to Cs-CysNPs-DOX and free DOX, while no cytotoxicity effects were found for free NPs. The caspase-3 activity showed that apoptosis was enhanced by raising the concentration of DOX, and the free NPs did not exhibit any caspase-3 activity. Overall, the developed HA/Cs-CysNPs-DOX displayed a high potential for targeted therapy and effective applications in biomedical investigation.

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“…the variation of the values of the nano sized depending on to the type and the nature of material applied. [20,21] Zeta Potential Study Zeta potential is a significant parameter representing the stability of drug particles measured, i.e. not change in chemical structure or physical modification of drugs (5-FU), (DOX) and (CA).…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Assessment of the in vitro controlled release for some polymeric nanocomposites loaded with different drugs

et al. 2020

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S yNthesIzeD starch cellulose acetate coacrylate (SCACA)/ Sodium bentonite modified Tween-80 (MSB) nanocomposites holding anticancer drugs like 5-fluorouracil (5-FU), Doxorubicin hydrochloride (DOX), and Chlorambucil (CA) as controlled release systems were evaluated and assessment as antiproliferative activity towards breast cancer cell lines, and antimicrobial activity against some strains. the nanocomposites loaded drugs characterized by Zeta Potential, FTIR, SEM and TEM. The results indicated that no change in the chemical structures and physical properties of the drugs into the nanocomposites. the surface texture of the samples was homogenous and smooth with no evidence of aggregations after release and there were some pore like structure and cracks formed due to blooming of drug outside nanocomposites. the particle size diameter of the prepared polymer was found to be 73 ~ 79 nm. After holding Doxorubicin the particle size was from 18 to 24 nm , 5-fluorouracil from 15 to 18 nm and Chlorambucil was from 24 to 75 nm. the release of drug was measured spectrophotometrically. the values of drug released depend on the type and nature of drug as well as the environmental aqueous media. the release in alkaline and acidic media was more faster and higher than that in the neutral one. the invitro study results of anticancer drugs released from polymers placed inside the culture media, showed 5-FU was given an effective promising results compared to DOX and CA. The antimicrobial test revealed that, the released 5-FU gave the highest effect as antimicrobial against all tested strains followed by DOX and CA.

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Amino acids combined chitosan nanoparticles for controlled release of Doxorubicin hydrochloride

Cited by 11 publications

References 32 publications

Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma

Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma

Redox-Sensitive Targeted Doxorubicin-Loaded Chitosan-based Nanoparticles to Treat Breast Cancer

Assessment of the in vitro controlled release for some polymeric nanocomposites loaded with different drugs

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