Complementing to our previous studies in biopolymers and amino acids for medical applications this study was addressed to design a unique therapeutic regime consisting of a cocktail from therapy with amino acids (TAAI) and polymer therapy for cancer disease. This regime is depending on synthesized Doxorubicin loaded chitosan-glutamic acid (Cs-Ga-DOX) nanoparticles via ionic gelation pathway. The encapsulation efficiency was 69%. The average size was 20-37 nm with spherical, homogenous structure, and positive zeta potential. FTIR of Cs-Ga confirmed the formation of amide linkage at 1644 cm-1. The in-vitro release of DOX was examined for both pH 5.5 and 7.4 to be biphasic with mutual burst release followed by sustained release for 168 h to reach 58% at pH 5.5 and 25% at pH 7.4. This result suggested that Cs-Ga nanoparticles presented a promising mixture of glutamic amino acid and chitosan as pH-responsive nano-carrier for anticancer drugs.