Amino acids in chitin — glucan complex ofAspergillus niger

Leštan, M; Pečavar, A.; Leštan, Domen; Perdih, Andrej

doi:10.1007/bf00805812

Cited by 4 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data provide indirect evidence of oxygen deficiency in the culture medium. The yield of lignolytic enzymes often increases after the nonionic detergents Tween 20 and Tween 80 are added to the culture medium [23][24][25]. In our experiments, various regimens of treatment of medium II with Tween 80 had no effect on the enzyme yield.…”

mentioning

confidence: 56%

New Approaches to Increasing the Yield of Laccase from Panus tigrinus

Chernykh

Leont'evskii

Golovleva

2005

Appl Biochem Microbiol

View full text Add to dashboard Cite

Laccase (EC 1.10.3.2, n -diphenol: oxygen oxidoreductase) is a copper-containing blue oxidase found in plants, fungi, and bacteria. This enzyme catalyzes single-electrode oxidation of various substrates (particularly phenols and aromatic amines) and reduces oxygen to water [1][2][3].Lignolytic fungi cause white rot. They differ from other wood-destroying fungi incapable of degrading lignin in the production of extracellular laccase. In addition, fungal laccase is involved in biosynthesis of conidial pigments (in ascomycetes) [4], fruit body pigments [5], and extracellular pigments that paste cell walls in hyphae during the formation of fruit bodies and rhizomorphs [2]. Laccase serves as a virulence factor in pathogenic fungi. Laccase of soil fungi and stratobios plays a role in the formation of soil humus [2,6,7].Laccase possesses no specificity, which allows this enzyme to be used for the oxidation of various compounds. Due to this feature, the enzyme is widely used as a tool to develop a variety of biotechnological methods. However, laccases are characterized by low values of the redox potential (in actinomycetes and basidiomycetes, they are equal to 400 and 750 mV, respectively, relative to the standard hydrogen electrode), which limits their use for oxidation of many stable compounds. The discovery of the phenomenon of mediation, which consists in the elevation of the overall redox potential of the laccase-mediator system, removed this restriction [8]. Taking into account these data, the simplicity of use (no need in cofactors), and stability, fungal laccases have gained wide acceptance as natural oxidizers in modern biotechnological methods for bleaching of paper stock and textile materials and for elimination of biocides, dyes, and other xenobiotics from sewage and natural water reservoirs. Increasing interest in the use of laccases in practice has resulted in intensive study of the conditions for increasing enzyme production.The yield of laccases from fungi exposed to submerged cultivation can be increased by changing the composition of the nutrient media, treatment with organic (phenol and non-phenol compounds) and inorganic inducers (copper and/or manganese ions), and the application of special methods for cultivation of producer fungi (e.g., immobilization of the mycelium) [12,13]. A specific feature of fungal laccase production is the absence of general inducers and other conditions that would be equally appropriate for various producing fungi.To increase the yield of laccase of the lignolytic fungus Panus tigrinus , we developed conditions for its cultivation involving Kirk's mineral medium [14,15] and biotechnological approaches to using free or immobilized laccase from P. tigrinus for delignification/bleaching of flax fibers [16] and purification of chlorophenolcontaminated water [17]. Recent studies have resulted in the appearance of new approaches to increasing the yield of fungal laccases. These data form the basis for the development of a new method of obtaining laccase from P. tigrinus .This w...

show abstract

mentioning

confidence: 56%

New Approaches to Increasing the Yield of Laccase from Panus tigrinus

Chernykh

Leont'evskii

Golovleva

2005

Appl Biochem Microbiol

View full text Add to dashboard Cite

show abstract

Chitin and Chitosan in Fungi

Peter¹

2002

Biopolymers Online

View full text Add to dashboard Cite

Introduction Chemical Structure Chitin Chitosan Polyphenolic Pigments Occurrence Physiological Function Chemical Analysis and Detection Biosynthesis of Chitin and Chitosan Chitin Synthases (CS) Glucan Transferase Chitin Deacetylase (CDA) Biodegradation Chitinase Chitosanases Exo‐β‐ d ‐glucosaminidases Biotechnological Production Screening for Chitosan Producer Strains Isolation of Chitin and Chitosan from Fungal Biomass Production of CDA Applications Adsorption of Coloring Matters Metal Ion Adsorption Healthcare Outlook Patents Acknowledgements

show abstract

Sonication of chitin–glucan, preparation of water-soluble fractions and characterization by HPLC

Mislovičová

Masárová²,

Bendžálová³

et al. 2000

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

A water-insoluble chitin-glucan complex, isolated from the mycelium of Aspergillus niger, was swollen in various aqueous media and treated subsequently by high-energy sonication. The concentration of the resulting water-soluble polysaccharide fractions was dependent on the swelling medium, the amount of the chitin-glucan complex in the suspension, and on the time of sonication. The yields of water-soluble chitin-glucan were within the range 13.6 to 24.4% relative to the mass of the original chitin-glucan. The nitrogen content obtained for the samples of water-soluble chitin-glucan indicated a higher content of chitin (3.45% of nitrogen in high-molecular fraction) than in the original water-insoluble chitin-glucan sample (1.8%). The distribution of the molecular weights of the water-soluble fractions prepared was determined by high-performance liquid chromatography.

show abstract

Amino acids in chitin — glucan complex ofAspergillus niger

Cited by 4 publications

References 7 publications

New Approaches to Increasing the Yield of Laccase from Panus tigrinus

New Approaches to Increasing the Yield of Laccase from Panus tigrinus

Chitin and Chitosan in Fungi

Sonication of chitin–glucan, preparation of water-soluble fractions and characterization by HPLC

Contact Info

Product

Resources

About