Amino acids requirements of measles virus in hela cells

Key Parameters of Measles Virus Production for Oncolytic Virotherapy

“…The results were normalized to titers produced in EBM supplemented with all amino acids and set as 100%. The production of MV in this reference medium reached 4.6×10 4 TCID 50 mL −1 (Romano and Scarlata, 1973).…”

“…The maximum virus yield obtained with the Edmonston strain has been determined by different groups, while various cell lines were infected with this MV strain (Black, 1959;Kohn and Yassky, 1962;Nakai et al, 1969;Romano and Scarlata, 1973;Baczko and Lazzarini, 1979;Brandriss et al, 1982;Scott and Choppin, 1982;Mendonca et al, 1994; (Table 2).…”

“…Black (1959) gained a maximum titer of 6.3×10 5 pfu mL −1 with Hep-2 cells, whereas Romano and Scarlata (1973) described a maximum titer of 4.6×10 4 TCID 50 mL −1 in HeLa cells and Udem (1984) a maximum of 1×10 8 pfu/mL in HeLa cells. In KB cells, a maximum titer of 5.6×10 6 TCID 50 mL −1 was obtained (Kohn and Yassky, 1962).…”

“…However, besides the parameters cell types and culture mode, the medium supplements have also been varied during the three culture set-ups (Baczko and Lazzarini, 1979). Thus, no absolute comparison is feasible, since the impact of medium composition on MV production yields has already been documented, as well (Romano and Scarlata, 1973;Scott and Choppin, 1982). Taken together, these data may be helpful for choosing an optimal MV producer cell line, but have to be interpreted with respect to the composition of the medium.…”

“…The amino acid requirements for MV production have been examined in HeLa cells, as well (Romano and Scarlata, 1973). For that purpose, the omission or addition of certain amino acids (tryptophan, cysteine, tyrosine, leucine, valine, phenylalanine, isoleucine, threonine, methionine, lysine, histidine, glutamine and arginine) was analyzed by comparing plaque formation in the infected cells.…”