Viral Vaccine Production in Cell Culture

Auniņš, John G.

doi:10.1002/9780470054581.eib582

Cited by 8 publications

(7 citation statements)

References 562 publications

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“…This cell population corresponds to the central point of BHK-21 exponential growth phase in suspension culture with the same culture medium, both for bioreactor (1L working volume) and spinner flask (Fernández-Núñez et al 2014). In general, cells in good nutritional as well proper enzymatic and growth conditions, with abundant intracellular and extracellular substrate maximize virus production (Auninš 2010) and probably protein expression of ''suicidal'' recombinant virus like SFV-RVGPV. This justifies the choice of 48 h as time of infection.…”

Section: Discussionmentioning

confidence: 99%

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Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension

et al. 2015

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Section: Discussionmentioning

confidence: 99%

“…These cell lines can be cultured in suspension. Specifically, the BHK-21 cell line has also been employed extensively for veterinarian viral vaccines (foot-and-mouth disease virus and rabies virus) since the 1960s (Auninš 2010).…”

Section: Introductionmentioning

confidence: 99%

Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension

et al. 2015

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“…The effect of aminoglycosides on The 24 hrs-37ºC infectivity titers expressed as TCID50/ml of FMD viruses treated by different concentrations of aminoglycosides antibiotics starting as 10 8 TCID50/ml revealed that all the aminoglycosides in this study affect infectivity titers (expressed as TCID50) except streptomycin (at concentrations ≤ 500 mg/L) as shown in table (2). , 1980,1983, Macintyre et al, 1991a,b, Ghendon et al, 1981and Gatti et al, 1998 who mentioned that the neomycin inhibit RND viruses replication and viral entry.…”

Section: Resultsmentioning

confidence: 60%

Study of In-Vitro Antiviral Activity of Aminoglycosides on Foot and Mouth Disease Virus

Mohamed¹

2019

ARC Journal of Animal and Veterinary Sciences

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Background: The aminoglycosides family of antibiotics are widely used as a prophylactic measure against bacterial contamination in animal vaccine production. Objective: Screening the antiviral activity of some members of aminoglycosides on foot and mouth disease (FMD) viruses used in the preparation of FMD vaccine in veterinary serum and vaccine research institute (VSVRI). Method: This study was conducted by in-vitro assessment of cytopathic effect (CPE) and infectivity titers of FMD viruses on Baby hamster kidney (BHK) cells under different concentrations of Neomycin (200, 500 mg/L), kanamycin (200, 500 mg/L), gentamycin (200, 500 mg/L) and streptomycin (200, 500 mg/L) as members of aminoglycosides using the inverted microscope. Positive (aminoglycosides-free) and negative (FMD viruses-free) were used. Results: Our results show that aminoglycosides antibiotics could inhibit the cytopathic effect and reduce the infectivity titers of FMD viruses. Conclusion: Finally we recommend the avoidance of routine inclusion of aminoglycosides antibiotics in tissue culture technique or treating FMD virus suspensions.

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“…human cell lines (HEK293, HT-1080) (Kantardjieff and Zhou, 2014;Zhu, 2012). Among these cell lines, BHK-21 has found applications at large scale in veterinarian viral vaccines against foot-and-mouth disease and rabies virus, as well as heterologous protein production (Factor VIII) (Auniņš, 2010;Palomares and Ramírez, 2009).…”

Section: Introductionmentioning

confidence: 99%

Model Comparison to Describe BHK-21 Cell Growth and Metabolism in Stirred Tank Bioreactors Operated in Batch Mode

Sartori

Leme

Caricati

et al. 2018

Braz. J. Chem. Eng.

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Baby Hamster Kidney cells (BHK-21) are commonly used in research and the biopharmaceutical industry. This work aimed to model the kinetic performance in batch operation mode of BHK-21 cells cultured in two stirred tank configurations using different dissolved oxygen concentrations and pH control strategies. Viable and dead cell concentrations, as well as glucose, glutamine, lactate and ammonium concentrations, were monitored. Statistical multiple linear regression, logistic equation and multiplicative Monod kinetic models were fitted. Statistical models for viable cells concentration as a function of nutrient and metabolite concentrations were significant (R 2 >0.91). Logistic model parameters: intrinsic growth rate, cell density level in the medium and time for reaching maximum cell concentrations were within 0.061-0.083 h-1 , 1.85-5.39 x 10 9 cell L-1 and 52-90 h ranges, respectively. A Monod-type model was satisfactorily fitted to the experimental data. Relative errors were lower than 10% for six monitored state variables in most of the assessed experimental conditions. The three models developed in this work can be used in bioprocesses involving BHK-21 with good fitting.

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Viral Vaccine Production in Cell Culture

Cited by 8 publications

References 562 publications

Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension

Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension

Study of In-Vitro Antiviral Activity of Aminoglycosides on Foot and Mouth Disease Virus

Model Comparison to Describe BHK-21 Cell Growth and Metabolism in Stirred Tank Bioreactors Operated in Batch Mode

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