Amino‐Functionalized Cellulose Nanoparticles: Preparation, Characterization, and Interactions with Living Cells

Abstract

Spherical nanoparticles with sizes from 80 to 200 nm are obtained by self-assembly of highly functionalized 6-deoxy-6-(ω-aminoalkyl)aminocellulosecarbamates. The particles are very stable, nontoxic, and possess primary amino groups that are accessible to further modifications in aqueous suspension. The particles can be labeled with rhodamine B isothiocyanate without changing their size, stability, and shape. The nanoparticles obtained are investigated by means of photo correlation spectroscopy, zeta potential measurements, SEM and fluorescence spectroscopy. Incorporation of the nanoparticles in human foreskin fibroblasts BJ-1-htert and breast carcinoma MCF-7 cells without any transfection reagent is proved by means of confocal laser scanning microscopy.

Biocompatibility and Antibacterial Effects of 6-Deoxy-6-Aminoethyleneamino Cellulose

“…AEAC with low DS exhibited the lowest anticoagulant and hemolytic potential of AEAC tested. In addition to AEAC solutions, also applications of corresponding nanoparticles are possible [4] [6].…”

“…A choice could Journal of Biosciences and Medicines be the use of chemically modified cellulose such as 6-deoxy-6-amino cellulose derivatives. A typical example of this novel class of cellulose derivatives is 6-deoxy-6-aminoethyleneamino cellulose (AEAC) [3] [4] [6]. AEAC is described to be biocompatible [2] and hemocompatible [7].…”

Tailor-made functional surfaces based on cellulose-derived materials

“…For instance, the immobilization of cationic compounds, such as Girard's reagent T or aminoguanidine, can be realized via the reaction with surface-attached aldehyde groups (Sirviö et al 2014). Nikolajski et al (2012) described the introduction of cationic groups in cellulose backbone through synthesis of aminocellulose via the nucleophilic displacement of tosyl groups by di-or trifunctional amines. Nanocapsules of aminocellulose showing cationic characters revealed antimicrobial capacity against E. coli (Fig.…”

Water soluble photoactive cellulose derivatives: synthesis and characterization of mixed 2-[(4-methyl-2-oxo-2H-chromen-7-yl)oxy]acetic acid–(3-carboxypropyl)trimethylammonium chloride esters of cellulose

“…In particular, cationic polyelectrolytes derived from polysaccharides display unconventional properties and are currently in the focus of research as flocculation agents in several industrial fields (Sirvio et al 2011), for self assembly onto different surfaces (Berlin et al 2003;Schwikal et al 2011), and for delivery of therapeutic or imaging agents (Yang et al 2012;Nikolajski et al 2012). In the present work, we describe the homogeneous preparation of photosensitive esters of cellulose containing 2-[(4-methyl-2-oxo-2H-chromen-7-yl)oxy]acetic acid and (3-carboxypropyl)trimethylammonium chloride ester moieties.…”