Amino-terminal and carboxy-terminal sequence of hepatic microsomal cytochrome P-450d, a unique hemoprotein from rats treated with isosafrole

Mouse cytochrome P3-450: complete cDNA and amino acid sequence

“…P3-450 mRNA has been determined to be approximately 2100 nucleotides, including the poly(A+) tail (40 cDNA's, the codon usage frequency phased from either position 2 or position 3 showed several striking exceptions to that found in normal mouse cDNA. Moreover, the first 25 amino acids correspond exactly to the NH2-terminal sequence of rat P-450d protein (41). Three of the five predicted COOterminal amino acids for mouse P3-450 (Fig.…”

“…Three of the five predicted COOterminal amino acids for mouse P3-450 (Fig. 2) correspond to those of the rat P-450d protein, whereas no such correspondence exists with the COOterminal amino acids for rat P-450c protein (41 (10,16). A 39-nucleotide sequence associated with the "highly conserved tridecapeptide" (9-11, 16, 18, 42) has been described in every P-450 protein or cDNA sequence studied to date and is known to be located in the center of exon 7 in the P-450b (10) and P-450e (11) genes.…”

“…The six cysteines of mouse P3-450 contrast with five estimated for rat P-450d protein (41). Most of the P3-450 amino acids are one or more greater than those estimated for rat P-450d protein (41); the largest differences include valine [40 for P3-450 and 32 for P-450d] and tryptophan [eight for P3-450 and four for P-450d].…”

Purification and characterization of a previously unreported form of cytochrome P-448 from the liver of 3-methylcholanthrene-pretreated rats

“…The constitutive isoenzymes, cytochromes P-450 RLM 3 and RLM 5, were reported by Cheng & Schenkman (1983). The sequence for cytochrome P-450d was reported by Botelho et al (1982).…”

Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops

“…This isosafrole-inducible form is almost certainly identical to a minor aromatic hydrocarboninducible form identified by purification from rats induced with hexachlorobiphenyl (Goldstein et al, 1982;Parkinson et al, 1983). Amino-terminal sequence analysis indicates no homology with the major PB-, 3MC-or isosafrole-inducible cytochrome P-4S0 isozymes (Botelho et al, 1 979;Botelho et al, 1982). Several "endogenous" cytochrome P-4S0 has also been resolved from microsomes after various treatments Cheng and Schenkman, 1982;Guenger ich et al, 1982a;.…”