Aminoglycoside Interference with the Dade Behring Pyrogallol Red–Molybdate Method for the Measurement of Total Urine Protein

Koerbin, Gus; Taylor, Lindsay G.; Dutton, Jacqui; Marshall, Katy; Low, Patricia; Potter, Julia M.

doi:10.1093/clinchem/47.12.2183

Cited by 15 publications

(2 citation statements)

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“…At the end of the study period, urine was collected for 24 h from each rat to be subjected to determination of protein according to Koerbin et al [22] and creatinine according to Larsen [23] with calculation of creatinine clearance. Blood was withdrawn from each rat to be subjected to determination of plasma levels of creatinine [23] and urea [24] in addition to the activities of lactate dehydrogenase (LDH) according to Zimmerman and Weinstein [25], aspartate transaminase (AST) and alanine transaminase (ALT) according to Reitman and Frankel [26], alkaline phosphatase (ALP) according to Bessey et al [27] and Gama-GT (γ- GT) according to [28] as well as the levels of total protein and albumin according to Rheinhold [29] and Doumas et al [30] in succession with calculation of globulin and albumin/globulin (A/G) ratio.…”

Section: Methodsmentioning

confidence: 99%

Hepatorenal protective effect of flaxseed protein isolate incorporated in lemon juice against lead toxicity in rats

2020

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Section: Methodsmentioning

confidence: 99%

Hepatorenal protective effect of flaxseed protein isolate incorporated in lemon juice against lead toxicity in rats

2020

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“…There are findings that aminoglycoside antibiotics, including gentamicin, can interfere with this assay, but also in urine samples. 37,38 Likewise, most of the tested parameters can be measured in plasma samples, so it would be useful to test this as well. Advantages of plasma are that it enables laboratories to achieve faster turnaround time and it is a preferred sample for point-of-care testing.…”

Section: Discussionmentioning

confidence: 99%

Gentamicin and Vancomycin Interference on Results of Clinical Chemistry Parameters on Abbott Architect c8000

Brenčić¹,

Nikolac²

2019

Archives of Pathology &Amp; Laboratory Medicine

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Context.— Gentamicin and vancomycin are nephrotoxic antibiotics. Little is known about the influence of drug concentrations on results of clinical chemistry tests. Objective.— To investigate gentamicin and vancomycin interference on results of 33 commonly measured biochemistry tests. Design.— The study was carried out in the University Department of Chemistry, Medical School University Hospital Sestre Milosrdnice (Zagreb, Croatia). For each drug, 10 aliquots of pooled serum were prepared. In order to cover toxic concentrations, pool serum samples were spiked with drugs to obtain 0 to 50 μg/mL of gentamicin and 0 to 200 μg/mL of vancomycin. Biochemistry tests were measured in duplicate on the Architect c8000 analyzer, and drug concentrations were measured on Architect i2000 SR (both Abbott Laboratories, Abbott Park, Illinois). For each tested concentration, bias was calculated against the initial measurement. Acceptance criteria were defined as measurement uncertainty of the commercial control with the value close to the measured range of the pool sample. Results.— For gentamicin, all bias values were below established criteria. For vancomycin, significant changes were observed for potassium, direct bilirubin, and immunoglobulin A. Significant bias was already detected at low vancomycin concentration (2.98 μg/mL) for direct bilirubin (bias = 9.7%; acceptable = 8%). Potassium bias at the highest vancomycin concentration (204.4 μg/mL) exceeded acceptance criteria (bias = 4.5%; acceptable = 4%). For immunoglobulin A, no apparent trend was observed, and bias is attributed to increased method imprecision. Conclusions.— Gentamicin did not interfere with the results of clinical chemistry tests. Direct bilirubin concentration is falsely increased in the presence of vancomycin, and potassium is affected at high concentrations.

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Biomarkers of Acute Kidney Injury

Dieterle¹,

Sistare²

2010

Biomarkers

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Aminoglycoside Interference with the Dade Behring Pyrogallol Red–Molybdate Method for the Measurement of Total Urine Protein

Cited by 15 publications

References 1 publication

Hepatorenal protective effect of flaxseed protein isolate incorporated in lemon juice against lead toxicity in rats

Hepatorenal protective effect of flaxseed protein isolate incorporated in lemon juice against lead toxicity in rats

Gentamicin and Vancomycin Interference on Results of Clinical Chemistry Parameters on Abbott Architect c8000

Biomarkers of Acute Kidney Injury

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