Aminoguanidine prevents testicular damage-induced-2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in male rats

Abstract

Summary In this study, it was aimed to determinate protective effects of aminoguanidine (AG) against reproductive toxicity caused by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD), an environmental contaminant. Thirty‐two rats were equally divided into four groups; the first group was kept as control and given corn oil as carrier. In second and third groups, TCDD and AG were orally administered at the dose of 2 μg kg−1 per week and 100 mg kg−1 per day for 45 days, respectively. In fourth group, TCDD and AG were given together at the same doses. Although TCDD significantly increased the formation of TBARS, it caused a significant decline in the levels of GSH, CAT, GPx and SOD in rats. On the other hand, AG, given together TCDD, reversed TCDD effects on TBARS SOD, GSH, GPx and CAT. In addition, sperm characteristics negatively affected and histopathological deformation occurred with TCDD exposure. However, AG treatment partly prevented these toxic effects of TCDD on spermatological parameters and histopathological changes. In conclusion, TCDD exposure induces testicular damage (oxidative stress, histopathological damage and sperm parameters), and AG treatment reversed TCDD‐induced testicular damage in rats. Thus, AG may be useful for the prevention and treatment of TCDD‐induced male infertility problems.

A Longitudinal Study of Peripubertal Serum Organochlorine Concentrations and Semen Parameters in Young Men: The Russian Children’s Study

“…Our findings are in agreement with animal data showing TCDD inhibition of testicular development and function during critical periods of reproductive-tract development, including fetal, neonatal ( Arima et al 2009 ; Faqi et al 1998 ), pubertal ( el-Sabeawy et al 1998 ), and adult stages ( Oguz et al 2013 ; Sönmez et al 2011 ). Moreover, childhood exposures to dioxins, furans, and PCBs have been shown to adversely affect other key maturational processes, such as somatic growth and pubertal timing in our cohort ( Burns et al 2011 , 2016 ; Korrick et al 2011 ).…”

Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress

“…In this sense, Balercia et al [43] found that asthenozospermic men show greater levels of NO • than normozoospermic men and that the concentration of this gasotransmitter were negatively correlated with the sperm motility. In this way, our findings indicate that aminoguanidine can be employed for protecting against the effects of oxidative stress in sperm cells, which is consistent with the findings in other cells and tissues (lung: [44]; bladder: [45]; kidney: [46]; testis: [29]). Similarly, Abbasi et al [30,31] and Alizadeh et al [32,33] found that the in vivo administration of aminoguanidine improves the sperm concentration, motility, viability, normal morphology, mitochondrial membrane potential, and DNA integrity in varicocelized rats where the upregulation of the iNOS isoform may lead to high levels of ROS in the semen.…”

“…In a recent study conducted by our research group [28], we found that aminoguanidine improves some sperm kinetic parameters during boar semen storage at 17 °C. Moreover, aminoguanidine protects against the negative effects of oxidative stress induced by environmental pollutants [29] and pathological conditions, such as varicocele [30,31,32,33]. Nonetheless, the in vitro effects of aminoguanidine on sperm cells under induced oxidative stress are still unknown.…”

Beneficial effects of aminoguanidine on radiotherapy-induced kidney and testis injury

“…More recently, Oguz et al . () declared that AG treatment reversed 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD)‐induced testicular damage in rats. The protective effect of AG has been thought to be related to its effects on reduction in free‐radical production and amelioration in antioxidant contents in the renal and testis tissue.…”