Aminolevulinic acid-loaded Witepsol microparticles manufactured using a spray congealing procedure: implications for topical photodynamic therapy

Al-Kassas, Rasil; Donnelly, Ryan F.; McCarron, Paul A.

doi:10.1211/jpp.61.09.0001

Cited by 13 publications

(3 citation statements)

References 48 publications

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“…After that, 1 mL of ALA solution was mixed with 200 µL of polyLys-Cyslinked PPA@AgNDs, and the system pH was adjusted to pH 7.4 with 1 N NaOH solution. The resultant solution was dialyzed for 1 h with a dialysis cassette (MWCO: 10 KDa) in PBS solution (pH 7.4) to remove unbound ALA. After the synthesis of PPA@AgND-ALA, the unbound ALA was collected and its concentration in solvent was determined using a method reported by AI-Kassas et al 34 ALA loading was calculated from:…”

Section: Electrostatic Adsorption Of 5-aminolevulinic Acid (Ala)mentioning

confidence: 99%

<p>Amylase-Protected Ag Nanodots for in vivo Fluorescence Imaging and Photodynamic Therapy of Tumors</p>

Wen

Wang²,

Liu

et al. 2020

IJN

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Background: Fluorescent metallic nanodots (NDs) have become a promising nanoprobe for a wide range of biomedical applications. Because Ag NDs have a high tendency to be oxidized, their synthesis and storage are a big challenge. Thus, the method for preparing stable Ag NDs is urgently needed. Surface modification and functionalization can enrich the capability of Ag NDs. Methods: In this work, fluorescent Ag NDs were synthesized in deoxygenated water by using porcine pancreatic α-amylase (PPA) as the stabilizing/capping agent. The absorption and fluorescence of PPA-protected Ag NDs (PPA@AgNDs) were measured with a spectrophotometer and a spectrofluorometer, respectively. The morphology of PPA@AgNDs was characterized by high-angle annular dark-field (HAADF) scanning transmission electron microscopy (STEM). The biocompatibility of PPA@AgNDs was evaluated by tetrazolium (MTT)-based assay. PolyLys-Cys-SH (sequence: KKKKKKC) peptides were conjugated to PPA@AgNDs via heterobifunctional crosslinkers. PolyLys-Cys-linked PPA@AgNDs absorbed 5-aminolevulinic acid (ALA) by electrostatic interaction at physiological pH. The capability of tumor targeting was evaluated by intravenously injecting PPA@AgND-ALA into 4T1 breast cancer xenograft mouse models. Photodynamic therapy (PDT) against tumors was performed under 635 nm laser irradiation. Results: PPA@AgNDs emitted at 640 nm with quantum yield of 2.1%. The Ag NDs exhibited strong photostability over a long period and a fluorescence lifetime of 5.1 ns. PPA@AgNDs easily entered the cells to stain the nuclei, showing the capabilities of living cell imaging with negligible cytotoxicity. ALA-loaded PPA@AgNDs (PPA@AgND-ALA) presented the superiority of passive tumor targeting via the enhanced permeability and retention (EPR) effect. Tumors were visualized in the near-infrared (NIR) region with reduced background noise. ALA molecules released from PPA@AgND-ALA was converted into the photosensitizer (PS) of protoporphyrin IX (PpIX) intracellularly and intratumorally, which greatly improved the PDT efficacy. Conclusion: Our approach opens a new way to design a novel theranostic nanoplatform of PPA@AgND-ALA for effective tumor targeting and fluorescence image-guided PDT.

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Section: Electrostatic Adsorption Of 5-aminolevulinic Acid (Ala)mentioning

confidence: 99%

<p>Amylase-Protected Ag Nanodots for in vivo Fluorescence Imaging and Photodynamic Therapy of Tumors</p>

Wen

Wang²,

Liu

et al. 2020

IJN

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“…The use of the spray cooling technique for the production of lipid particles for topical use is still a minorly explored area of research [108]. Some studies have investigated the feasibility and efficiency of the production of solid lipid micro/nanoparticles through the spray colling technique for active ingredients with potential topical use [110][111][112][113].…”

Section: Application Of Films In Cosmetics and Dermatologymentioning

confidence: 99%

Lipid-Polymeric Films: Composition, Production and Applications in Wound Healing and Skin Repair

et al. 2021

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The use of lipids in the composition of polymeric-based films for topical administration of bioactive ingredients is a recent research topic; while few products are commercially available, films containing lipids represent a strategic area for the development of new products. Some lipids are usually used in polymeric-based film formulations due to their plasticizing action, with a view to improving the mechanical properties of these films. On the other hand, many lipids have healing, antimicrobial, anti-inflammatory, anti-aging properties, among others, that make them even more interesting for application in the medical-pharmaceutical field. This manuscript discusses the production methods of these films both on a laboratory and at industrial scales, the properties of the developed biopolymers, and their advantages for the development of dermatologic and cosmetic products.

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“…There is a tremendous potential for nanoparticle‐driven drug delivery, which has led to a huge swell of interest in this area. The many advantages of nanovehicles include their small size, customizable surface properties, tunable solubility, and multifunctionality (2, 65–73). They pave the way for new ground to break in biomedical applications.…”

Section: Therapymentioning

confidence: 99%

Photonanodermatology: the interface of photobiology, dermatology and nanotechnology

Hia

Nasir

2011

Photoderm Photoimm Photomed

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SummaryThis review focuses on the optical properties of matter on the nanoscale and discusses some of their potential applications in dermatology. The applications will be divided into three main categories: those with consumer potential; those with diagnostic potential; and those with therapeutic potential. N anotechnology is the exploration of the properties of matter on an infinitesimally small size scale. The range varies from 1 to 100 nm in some classifications and from 1 to 1000 nm in others. These ranges are larger than atoms and molecules and smaller than cellular organelles and viruses. Biologically, the nanoscale is very important, because it is the size range at which most life processes occur. Substances behave differently from their bulk precursors on the nanoscale. For example, brittle insulators, like glass, are soft and flexible and conduct electricity on the nanoscale. Motors, like flagellar rotors, can spin quite rapidly and generate a great deal of power. The goal of nanotechnology is to combine the properties of smallscale matter with purposeful design to generate a useful product. For example, a carbon nanotube cylinder by itself might have interesting properties, for example, high tensile strength, conductivity, and the ability to form covalent linkages with a variety of organic and inorganic moieties. If the nanotube were made uniformly and reproducibly, but served no purpose, other than conducting electricity, it would not be an example of nanotechnology (1). However, if it were coupled to an antibody, placed across an electrical gap, and its conductivity is measured in the presence or absence of antigen, it could be used to create a biosensor smaller than an organelle. This, then would be an example of nanotechnology. A nanoliposome of a small size and small carrying capacity would not be an example of nanotechnology. If such a liposome were designed to carry hydroxychloroquine, and target its release in T cells, this type of purposeful design meets the criteria of nanotechnology. This review focuses on the optical properties of matter on the nanoscale and discusses some of their potential applications in dermatology. The applications will be divided into three main categories: those with consumer potential; those with diagnostic potential; and those with therapeutic potential.

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Aminolevulinic acid-loaded Witepsol microparticles manufactured using a spray congealing procedure: implications for topical photodynamic therapy

Cited by 13 publications

References 48 publications

<p>Amylase-Protected Ag Nanodots for in vivo Fluorescence Imaging and Photodynamic Therapy of Tumors</p>

<p>Amylase-Protected Ag Nanodots for in vivo Fluorescence Imaging and Photodynamic Therapy of Tumors</p>

Lipid-Polymeric Films: Composition, Production and Applications in Wound Healing and Skin Repair

Photonanodermatology: the interface of photobiology, dermatology and nanotechnology

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