Abstract.A 50-day field study was carried out in a seminatural, non-fertilized grassland in south-western Ontario, Canada during the late summer and early autumn of 2012. The purpose was to explore surface-atmosphere exchange processes of ammonia (NH 3 ) with a focus on bi-directional fluxes between the soil and atmosphere. Measurements of soil pH and ammonium concentration ([NH . The soil compensation point, the atmospheric NH 3 mixing ratio below which net emission from the soil will occur, exhibited both a seasonal trend and diurnal trend. Higher daytime and August compensation points were attributed to higher soil temperature. Soil-atmosphere fluxes were estimated using NH 3 measurements from the Ambient Ion Monitor Ion Chromatograph (AIM-IC) and a simple resistance model. Vegetative effects were ignored due to the short canopy height and significant soil . Inferred fluxes were, on average, 2.6 ± 4.5 ng m −2 s −1 in August (i.e. net emission) and −5.8 ± 3.0 ng m −2 s −1 in September (i.e. net deposition). These results are in good agreement with the only other bi-directional exchange study in a semi-natural, non-fertilized grassland. A Lagrangian dispersion model (Hybrid Single-Particle Lagrangian Integrated Trajectory -HYSPLIT) was used to calculate air parcel back-trajectories throughout the campaign and revealed that NH 3 mixing ratios had no directional bias throughout the campaign, unlike the other atmospheric constituents measured. This implies that soil-atmosphere exchange over a non-fertilized grassland can significantly moderate nearsurface NH 3 concentrations. In addition, we provide indirect evidence that dew and fog evaporation can cause a morning increase of [NH 3 ] g . Implications of our findings on current NH 3 bi-directional exchange modelling efforts are also discussed.