Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Nold, Stephen C.; Boschker, Henricus T. S.; Pel, Roel; Laanbroek, Hendrikus J.

doi:10.1016/s0168-6496(99)00002-1

Cited by 23 publications

(27 citation statements)

References 39 publications

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“…In the freshly harvested sediment before [methyl‐ 13 C]toluene incubation, this compound was significantly depleted (−40.3‰) relative to averaged values of the total of PLFA (−28.9‰), reflecting CH 4 assimilation because biogenic CH 4 has a depleted carbon isotopic signal (−65 to −50‰). This depleted isotopic signal supported previous suggestions that 16:1ω5 is characteristic for group I methanotrophs [40]. However, it has been shown that 16:1ω5 is also commonly found in other Gram‐negative bacteria, including strain PRTOL1 (Table 3) and other genera of sulfate‐reducing bacteria [14,16].…”

Section: Discussionsupporting

confidence: 88%

Tracing toluene-assimilating sulfate-reducing bacteria using 13C-incorporation in fatty acids and whole-cell hybridization

Pelz

Chatzinotas

Zarda-Hess

et al. 2001

FEMS Microbiology Ecology

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Polar lipid-derived fatty acids (PLFA) commonly found in sulfate-reducing bacteria were detected in high abundance in the sediment harvested from a monitoring well of a petroleum-hydrocarbon (PHC)-contaminated aquifer. Aquifer microcosms were incubated under sulfate-reducing conditions with [methyl-14 C]toluene to determine the 14 C-mass balances and with [methyl-13 C]toluene to follow the flow of carbon from toluene into biomarker fatty acids. An aliquot was used to establish an aquifer-derived toluene-degrading sulfate-reducing consortium, which grew well in liquid medium. Whole-cell hybridization using 16S rRNA-targeted oligonucleotide probes specific for different phylogenetic levels within the sulfate-reducing bacteria was applied in order to characterize the sulfate-reducing populations in the original sediment, the aquifer microcosms, and the aquifer-derived consortium. In the aquifer microcosms, the 14 C quantification revealed that 61.6% of the [methyl-14 C]toluene was mineralized and 2.7% was assimilated. Following [methyl-13 C]toluene depletion ( 6 1 WM), the highest 13 C-enrichment was found in PLFA 16:1g5c. In addition, biomarker fatty acids characteristic for the genera Desulfobacter and Desulfobacula (cy17:0 and 10Me16:0) were also 13 C-enriched, contrary to those of other sulfate-reducing genera, e.g. Desulfovibrio and Synthrophobacter (i17:1g7c), Desulfobulbus and Desulforhabdus (15:1g6c and 17:1g6c). Although hybridization detection rates remained low, indicating low bacterial activities, 43% (aquifer sediment) and 30% (aquifer microcosm) of the total active bacteria belonged to the Desulfobacteriaceae thus supporting the PLFA-based results. Desulfobacter-species (42%), which belong to the Desulfobacteriaceae, dominated the community of the consortium. Our study showed that carbon stable isotope analysis in combination with whole-cell hybridization could link toluene degradation in aquifer microcosms to the metabolic activity of the Desulfobacter-like populations. These populations could play an important role in the clean up of aromatic PHC-contaminated aquifers. ß

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Section: Discussionsupporting

confidence: 88%

Tracing toluene-assimilating sulfate-reducing bacteria using 13C-incorporation in fatty acids and whole-cell hybridization

Pelz

Chatzinotas

Zarda-Hess

et al. 2001

FEMS Microbiology Ecology

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“…Groups of biomarker PLFA: gram‐positive (G+ve) bacteria (branched‐chained iso/anteiso FA), gram‐negative (G−ve) (monounsaturated and cyclic FA), actinobacteria (10Me‐branched FA) and fungi (18:2 ω 6,9) and non‐specific PLFA [16:0,18:0]. % 13 C incorporation calculated using I = ( F e − F c )[PLFA] c (after Nold et al , 1999). Error bars represent standard deviation ( n = 3).…”

Section: Resultsmentioning

confidence: 99%

“…δ 13 C values obtained for methylated compounds were corrected for the addition of derivative C using the mass balance equation: where n = number of C atoms, c = δ 13 C value of the underivatized compound, d = δ 13 C value of the derivatizing agent (BF 3 MeOH; bulk δ 13 C value −34.1‰, determined by continuous flow‐IRMS) and cd = δ 13 C value of the derivatized compound. The fractional abundance ( F ) of 13 C in the control ( F c ) and enriched ( F e ) PLFA was used to calculate the concentration of 13 C incorporated into PLFA from the total PLFA concentration, using the equation I = (Fe − Fc)[PLFA]c (after Nold et al , 1999). The fractional abundance expresses the concentration of 13 C as a proportion of the total concentration of carbon in the PLFA using the equation F = [ 13 C/ ( 13 C + 12 C )].…”

Section: Methodsmentioning

confidence: 99%

Variable responses of the soil microbial biomass to trace concentrations of ¹³C‐labelled glucose, using ¹³C‐PLFA analysis

Dungait

Kemmitt

Michallon

et al. 2010

European J Soil Science

139

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13 C-labelled glucose was applied to arable (Broadbalk NPK) and permanent grassland (Woburn Grassland) soils to investigate the response of the soil microbial biomass (SMB) to carbon (C) applied at trace concentrations. Phospholipid fatty acids (PLFA) were used as biomarkers for G+ve (odd-chained and iso/anteiso FA) and G−ve bacteria (mono-unsaturated and cyclic FA), actinobacteria (10-methyl-branched FA), fungi (octadecadienoic acid) and general membrane lipids [16:0,18:0]. Gas chromatography-combustion-stable isotope ratio mass spectrometry (GC-C-IRMS) was used to determine the incorporation of 13 C into individual PLFA in two experiments: first, after application of a single concentration (15 μg C g −1 ) of 13 C-glucose over a time sequence (0, 8, 24, 48, 120 and 240 hours), and second after application at three concentrations (25, 83, 416 μg C g −1 soil after 120 hours). 13 C incorporation into PLFA over time was similar in both soils. However, in the permanent grassland soil, 13 C incorporation was increased in actinobacteria PLFA at 120 hours when [16:0,18:0] was reduced. At 240 hours, 13 C incorporation increased in [16:0,18:0] concurrently with a reduction in G+ve bacteria PLFA. Increasing glucose concentration caused different responses in the SMB of both soils. In the arable soil, all biomarker PLFA concentrations increased at all rates of application. In contrast, in the permanent grassland soil PLFA concentrations were similar to the control in all SMB groups, except G+ve bacteria after the greatest rate of application. However, the δ 13 C values of the same PLFA indicated that uptake of applied 13 C-glucose was proportional to the applied concentration in all groups of soil bacteria in both soils.

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“…The fractional abundance expresses the concentration of 13 C as a proportion of the total concentration of C in the PLFA using the equation described by Nold, Boschker, Pel, and Laanbroek ()

normalF = ([], ^{13} normalC / ((), ^{13} normalC +^{12} normalC))

…”

Section: Methodsmentioning

confidence: 99%

Pyrolysis temperature during biochar production alters its subsequent utilization by microorganisms in an acid arable soil

et al. 2017

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Biochar amendment of agricultural soils can have a significant impact on microbial carbon (C) metabolism by providing C substrates and altering soil properties, including amelioration of soil acidity. It remains unclear whether available C of biochar or its pH effects determines the utilization of biochar by microorganisms. Compound‐specific stable 13C isotope analysis of phospholipid fatty acids (13C‐phospholipid fatty acid analysis) was used to explore which microbial group utilized biochar distinguished with pHs and C availability. C4 Miscanthus biochar (δ13C = −12.2‰) was prepared at 2 pyrolysis temperatures (350 °C and 700 °C) and applied (50 mg C g−1 soil) to a very acid soil (pH 3.7, δ13C = −27.7‰), which was sampled from the long term Hoosfield Acid Strip experiment at Rothamsted Research, and incubated for 14 months. Biochar700 increased soil pH to 5.1 and biochar350 increased soil pH to 4.3. All microbial groups (Gram‐positive bacteria, Gram‐negative bacteria, actinobacteria, and fungi) were more abundant in the biochar‐treated soils. The 13C values of biomarker phospholipid fatty acid analysis suggested that all groups of microorganisms, and especially Gram‐positive bacteria, were using the C from the biochar350, but not the biochar700, as a substrate. We conclude that its utilization of biochar by microorganisms after 14 months was largely determined by the pyrolysis temperature controlling the availability of biochar C, instead of the pH effects, in a very acidic soil.

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Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Cited by 23 publications

References 39 publications

Tracing toluene-assimilating sulfate-reducing bacteria using 13C-incorporation in fatty acids and whole-cell hybridization

Tracing toluene-assimilating sulfate-reducing bacteria using 13C-incorporation in fatty acids and whole-cell hybridization

Variable responses of the soil microbial biomass to trace concentrations of ¹³C‐labelled glucose, using ¹³C‐PLFA analysis

Pyrolysis temperature during biochar production alters its subsequent utilization by microorganisms in an acid arable soil

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Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Cited by 23 publications

References 39 publications

Tracing toluene-assimilating sulfate-reducing bacteria using 13C-incorporation in fatty acids and whole-cell hybridization

Tracing toluene-assimilating sulfate-reducing bacteria using 13C-incorporation in fatty acids and whole-cell hybridization

Variable responses of the soil microbial biomass to trace concentrations of 13C‐labelled glucose, using 13C‐PLFA analysis

Pyrolysis temperature during biochar production alters its subsequent utilization by microorganisms in an acid arable soil

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Variable responses of the soil microbial biomass to trace concentrations of ¹³C‐labelled glucose, using ¹³C‐PLFA analysis