Ammonium Gluconate Production from Potato Starch Wastewater Using a Multi-Enzyme Process

Chen, Bingcui; Kang, Li; Liao, Lingtong; Wang, Ruiming; Li, Piwu

doi:10.1007/s12230-019-09733-8

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…According to the manufacturer specifications, the activities of the GOx and catalase were 6.5 U•mg pure enzyme −1 and 2000 U• mg protein −1 , respectively, at 25 °C and pH 7. 20 However, the overall activity of the lyophilized catalase formulation may be lower since its protein content is only specified to be ≥60%. Even so, this suggests that the catalase concentration required to consume all the hydrogen peroxide produced by GOx should be fairly low in comparison to the concentration of GOx in the reactor, which can help to reduce costs.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The activity of an enzyme is measured in units (U), which are defined as the amount of enzyme required to convert 1 μmol substrate per minute. According to the manufacturer specifications, the activities of the GOx and catalase were 6.5 U·mg pure enzyme –1 and 2000 U·mg protein –1 , respectively, at 25 °C and pH 7 . However, the overall activity of the lyophilized catalase formulation may be lower since its protein content is only specified to be ≥60%.…”

Section: Materials and Methodsmentioning

confidence: 99%

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Modeling and Experimental Validation of Continuous Biocatalytic Oxidation in Two Continuous Stirred Tank Reactors in Series

Lindeque

Woodley

2022

Org. Process Res. Dev.

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The rate of oxygen transfer from the gas phase to the liquid phase is a critical process parameter for biocatalytic oxidations due to the poor water solubility of molecular oxygen and the low oxygen affinity of many of the relevant enzymes, such as oxidases. In gas–liquid systems, mechanical mixing can be used to increase the interfacial area available for mass transfer and thereby increase the volumetric mass transfer coefficient (k L a). As such, the operation of these reactions in a continuous stirred tank reactor (CSTR) may allow for better performance in a readily scalable way. Even so, achieving a high substrate conversion in a single reactor would require operation at high pressure, to improve the solubility of oxygen, as well as a high enzyme concentration. An alternative and more cost-effective means of improving the substrate conversion might be to operate a series of multiple CSTRs. As such, the oxidation of glucose to gluconic acid by glucose oxidase, coupled with catalase, was modeled in a series of identical well-mixed reactors. It was found that achieving full conversion would require an impractical number of reactors at atmospheric pressure. However, the overall conversion of the reaction could be doubled by simply using two CSTRs in series. Subsequently, experiments were carried out to validate this, and the results showed that the overall conversion was in fact tripled. This likely resulted from a higher k L a in the second reactor, which was potentially caused by the change in the media composition from the first reactor.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Modeling and Experimental Validation of Continuous Biocatalytic Oxidation in Two Continuous Stirred Tank Reactors in Series

Lindeque

Woodley

2022

Org. Process Res. Dev.

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“…Wheat starch-processing wastewater has potential as a rich source of pentosans. Furthermore, the recycling of wastewater can also improve the economic efficiency of factories and reduce pressure on waste water treatment facilities [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Biological modification of pentosans in wheat B starch wastewater and preparation of a composite film

Zhao

Wei

et al. 2022

BMC Biotechnol

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Background Petrochemical resources are becoming increasingly scarce, and petroleum-based plastic materials adversely impact the environment. Thus, replacement of petroleum-based materials with new and effective renewable materials is urgently required. Results In this study, a wheat pentosan-degrading bacterium (MXT-1) was isolated from wheat-processing plant wastewater. The MXT-1 strain was identified using molecular biology techniques. The degradation characteristics of the bacteria in wheat pentosan were analyzed. The results show that wheat pentosan was effectively degraded by bacteria. The molecular weight of fermented wheat pentosan decreased from 1730 to 257 kDa. The pentosan before and after the biological modification was mixed with chitosan to prepare a composite film. After fermentation, the water-vapor permeability of the wheat pentosan film decreased from 0.2769 to 0.1286 g mm (m2 h KPa)−1. Results obtained from the Fourier-transformed infrared experiments demonstrate that the wave number of the hydroxyl-stretching vibration peak of the membrane material decreased, and the width of the peak widened. The diffraction peak of the film shifted to the higher 2θ, as seen using X-ray diffraction. The cross-section of the modified composite membrane was observed via scanning electron microscopy, which revealed that the structure was denser; however, no detectable phase separation was observed. These results may indicate improved molecular compatibility between wheat pentosan and chitosan and stronger hydrogen bonding between the molecules. Given the increased number of short-chain wheat pentosan molecules, although the tensile strength of the film decreased, its flexibility increased after fermentation modification. Conclusion The findings of this study established that the physical properties of polysaccharide films can be improved using strain MXT-1 to ferment and modify wheat pentosan. The compatibility and synergy between pentosan and chitosan molecules was substantially enhanced, and hydrogen bonding was strengthened after biological modification. Therefore, modified pentosan film could be a potential candidate material for edible packaging films.

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Resource utilization and ionization modification of waste starch from the recycling process of old corrugated cardboard paper

Lingrui

Yang

et al. 2020

Journal of Environmental Management

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Ammonium Gluconate Production from Potato Starch Wastewater Using a Multi-Enzyme Process

Cited by 4 publications

References 32 publications

Modeling and Experimental Validation of Continuous Biocatalytic Oxidation in Two Continuous Stirred Tank Reactors in Series

Modeling and Experimental Validation of Continuous Biocatalytic Oxidation in Two Continuous Stirred Tank Reactors in Series

Biological modification of pentosans in wheat B starch wastewater and preparation of a composite film

Resource utilization and ionization modification of waste starch from the recycling process of old corrugated cardboard paper

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