Ammonium intensifies CAM photosynthesis and counteracts drought effects by increasing malate transport and antioxidant capacity in Guzmania monostachia

Pereira, Paula Natália; Gaspar, Marı́lia; Smith, J. Andrew C.; Mercier, Helenice

doi:10.1093/jxb/ery054

Cited by 23 publications

(25 citation statements)

References 64 publications

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“…Several studies have confirmed that the activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX), as well as the concentrations of hydrogen peroxide (H 2 O 2 ) are up‐regulated during the induction of CAM (Ślesak et al 2008, Nosek et al 2015). Furthermore, antioxidant enzymes activities exhibit differential diurnal rhythm during C 3 ‐CAM transition (Niewiadomska et al 2011, Habibi and Hajiboland 2012, Pereira et al 2018). In a previous study, it has been reported that the C 3 ‐CAM transition can induce the changes in antioxidative enzymes activities in a reversible manner (Nosek et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparison of CAM expression, photochemistry and antioxidant responses in Sedum album and Portulaca oleracea under combined stress

Habibi

2020

Physiologia Plantarum

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Previous studies of crassulacean acid metabolism (CAM) pathway during stress have been directed at individual drought and salinity stress, here, we studied the effects of a combination of drought and salt on CAM expression, chlorophyll fluorescence and antioxidant parameters in the C 3-CAM facultative Sedum album and C 4-CAM facultative Portulaca oleracea plants. While salinity alone was not able to induce functional CAM expression in P. oleracea leaves, we showed that salinity induced low level of nocturnal acid accumulation in S. album species. After 20 d of exposure to the combination of simultaneous salt and drought stress, P. oleracea plants exhibited more resistance to photoinhibition as compared to S. album plants. The decrease of maximum quantum yield (F v /F m) in S. album leaves under combined stress was in parallel with the largest suppression of CAM expression of >50%, probably displaying the withdrawal of functional CAM back to C 3 pathway. However, under drought treatment alone, S. album plants exhibited higher photosynthetic flexibility, which was associated with the up-regulation of antioxidant enzymes activities and maintenance of glutathione (GSH) pool, and consequently higher photochemical functioning. The levels of nitric oxide (NO) correlated well with CAM expression, which was observed only in S. album, suggesting that NO acts in a different way in C 3 and C 4 species during CAM induction. Additionally, in both species, over the course of CAM induction, the changes in CAM expression parameters exhibited a similar pattern to that of antioxidant capacity and photochemical functioning parameters.

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Section: Introductionmentioning

confidence: 99%

Comparison of CAM expression, photochemistry and antioxidant responses in Sedum album and Portulaca oleracea under combined stress

Habibi

2020

Physiologia Plantarum

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“…According to Han et al [70], under low temperature stress, the content of NO 3 --N and the NR activities in tomato leaves signi cantly decreased, while the NH 4 + -N content signi cantly increased. Under drought stress, the NH 4 + nutrition can limit the effect of water de cit by osmotic adjustment and can limit oxidative damage [71].…”

Section: Different Changes Of Nrt and Amt Family Gene Expression Pattmentioning

confidence: 99%

Adaptation of Cucumber Seedlings to Low Temperature Stress by Reducing Nitrate to Ammonium During It’s Transportation

Liu

Bai

Wang

et al. 2020

Preprint

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Background: Low temperature seriously depressed the uptake, translocation from root to shoot and metabolism of nitrate and ammonium in thermophilic plants such as cucumber, and the growth of plant was inhibited accordingly. However, there was no breakthrough in the effect of low temperature on nitrogen transport over the years. Results: By using the non-invasive micro-test technology the net NO3- and NH4+ fluxes rate in root hair zone and vascular bundles of main root, stem, petiole, midrib, lateral vein, and shoot tip of cucumber seedlings under normal temperature (NT) and low temperature (LT) treatment were tested, respectively. Under LT treatment, the net NO3- flux rate in root hair zone and vascular bundles of cucumber seedlings decreased, while the net NH4+ flux rate in vascular bundles of midribs, lateral veins and shoot tips increased. In accordance with this, the relative expression of CsNRT1.4a in petioles and midribs was down-regulated, while the expressions of CsAMT1.2a~1.2c in midribs were up-regulated. The results of 15N isotope tracing showed that compared with NT treatment, NO3--N and NH4+-N uptake of the seedlings under LT treatment decreased 78.1% and 58.8%, respectively, and the concentration and proportion of both NO3--N and NH4+-N distributed in the shoot decreased. Under LT treatment, the actual nitrate reductase activity (NRAact) in roots didn’t change significantly, while NRAact in stems and petioles of LT treatment increased by 113.2% and 96.2%, respectively.Conclusion: In summary, the higher net NH4+ flux rate in leaves and young tissues may be due to the higher NRact in stems and petioles, which could reduce more NO3- to NH4+ so as to reduce the energy consumption in nitrogen transportation under low temperature.

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“…The influence of NO 3 − on CAM expression has been documented in several obligate and facultative CAM species [43,45,70,71,72]. Researchers observed that K. blossfeldiana plants grown under 1 mM NO 3 − or NH 4 + despite having the same growth rates, showed higher nocturnal CO 2 uptake and nocturnal malate content in the presence of NO 3 − [70].…”

Section: Relationship Between Nitrogen and Cammentioning

confidence: 99%

“…, when G. monostachia was grown in the presence of 5.0 mM NO 3 − and exposed to water-deficit stress, the magnitude of CAM decreased by lowering nocturnal malate and citrate accumulation, PEPC and MDH activities, GmoALMT9 expression (aluminum-dependent malate transporter), and the rates of ATP and PPi-dependent proton transport when compared to plants grown in the presence of 5.0 mM NH 4 + and water-deficit stress. When lower concentrations of NO 3 − were evaluated (e.g., 1.25 mM and 2.5 mM), a slight increase in PEPC activity was observed; however, it was still lower than when compared to the same concentrations of NH 4 + [72]. Thus, the preference for each N source seems to depend upon the plant species, their habitat, and the relative toxicity of the N source for the plant.…”

Section: Relationship Between Nitrogen and Cammentioning

confidence: 99%

Exploring the Relationship between Crassulacean Acid Metabolism (CAM) and Mineral Nutrition with a Special Focus on Nitrogen

Pereira

Cushman

2019

IJMS

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Crassulacean acid metabolism (CAM) is characterized by nocturnal CO2 uptake and concentration, reduced photorespiration, and increased water-use efficiency (WUE) when compared to C3 and C4 plants. Plants can perform different types of CAM and the magnitude and duration of CAM expression can change based upon several abiotic conditions, including nutrient availability. Here, we summarize the abiotic factors that are associated with an increase in CAM expression with an emphasis on the relationship between CAM photosynthesis and nutrient availability, with particular focus on nitrogen, phosphorus, potassium, and calcium. Additionally, we examine nitrogen uptake and assimilation as this macronutrient has received the greatest amount of attention in studies using CAM species. We also discuss the preference of CAM species for different organic and inorganic sources of nitrogen, including nitrate, ammonium, glutamine, and urea. Lastly, we make recommendations for future research areas to better understand the relationship between macronutrients and CAM and how their interaction might improve nutrient and water-use efficiency in order to increase the growth and yield of CAM plants, especially CAM crops that may become increasingly important as global climate change continues.

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Ammonium intensifies CAM photosynthesis and counteracts drought effects by increasing malate transport and antioxidant capacity in Guzmania monostachia

Abstract: Leaves of Guzmania monostachia exposed to ammonium and water deficit showed increased CAM photosynthesis and water deficit tolerance by increasing vacuolar malate channel expression levels, antioxidant enzyme activities, and soluble sugar accumulation.

Cited by 23 publications

References 64 publications

Comparison of CAM expression, photochemistry and antioxidant responses in Sedum album and Portulaca oleracea under combined stress

Comparison of CAM expression, photochemistry and antioxidant responses in Sedum album and Portulaca oleracea under combined stress

Adaptation of Cucumber Seedlings to Low Temperature Stress by Reducing Nitrate to Ammonium During It’s Transportation

Exploring the Relationship between Crassulacean Acid Metabolism (CAM) and Mineral Nutrition with a Special Focus on Nitrogen

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