Amniotic ectoderm in tissue‐cultures

Lewis, W. Arthur

doi:10.1002/ar.1090260204

Cited by 5 publications

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“…Early studies of morphogenetic movements relied largely on tissue culture and optically accessible embryos such as the lancelet (amphioxus), chicken and Fundulus (killifish) for experimental manipulation (Abercrombie, 1977;Conklin, 1932;Harrison, 1910Harrison, , 1912Lewis, 1923;Spratt, 1948;Trinkaus, 1963). Subsequent planar cell culture studies, in which cellular motion was analyzed in the context of a static ECM scaffold, led to a common (and misleading) assumption that all migratory patterns observed in intact embryos arose via cells actively crawling through or upon the ECM (Bilozur and Hay, 1988;Hay, 1989).…”

Section: The Role Of Ecm In Tissue Morphogenesis: a Historical Overviewmentioning

confidence: 99%

Extracellular matrix motion and early morphogenesis

et al. 2016

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For over a century, embryologists who studied cellular motion in early amniotes generally assumed that morphogenetic movement reflected migration relative to a static extracellular matrix (ECM) scaffold. However, as we discuss in this Review, recent investigations reveal that the ECM is also moving during morphogenesis. Time-lapse studies show how convective tissue displacement patterns, as visualized by ECM markers, contribute to morphogenesis and organogenesis. Computational image analysis distinguishes between cell-autonomous (active) displacements and convection caused by large-scale (composite) tissue movements. Modern quantification of large-scale 'total' cellular motion and the accompanying ECM motion in the embryo demonstrates that a dynamic ECM is required for generation of the emergent motion patterns that drive amniote morphogenesis.

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