Amniotic Fluid Cells; Prenatal Sex Prediction and Culture

Abstract

, 1970, 1, 523-526 Summary: Sex chromatin studies were carried out on small amounts of amniotic fluid obtained by amniocentesis or from intact amniotic sacs removed at hysterotomy. Provided that satisfactory preparations were obtained the accuracy of fetal sexing was 87%. Nevertheless, in the management of a pregnancy in which there is a risk of a serious X-linked recessive disorder, repeat amniocentesis may be necessary to ensure satisfactory specimens.Of 90 samples of fluid cultured, satisfactory growth was obtained in 49; the success rate was not increased by the addition of stimulants to the culture medium. It is suggested that between the 13th and the 16th week of pregnancy is the optimum time for amniocentesis to obtain cells for culture, since sufficient cells are then present in a small volume of fluid and therapeutic abortion would still be possible once the results were available.

Possibility of culturing foetal cells at early stages of pregnancy

“…-Harvesting is made when the cells are in fewer than 2000 viable cells per ml of amniotic fluid before the 15th week, but Nelson & Emery (1970), comparing cases taken before and after the 20th week, could show no clear relationship between numbers of inoculated cells or gestational age and the success of cell culture. Gerbie et al (1971) and Valenti & Kehaty (1969) found the 14th and 16th weeks to be the optimum time for amniocentesis.…”

Análise dos resultados dos procedimentos invasivos para estudo do cariótipo fetal

“…O rastreamento para aneuploidias inicia-se nos anos setenta (28)(29)(30) , década em que a avaliação do risco para trissomias é baseada principalmente na idade materna e em antecedentes de fetos com anormalidades cromossômicas (29,(31)(32)(33) . (28) .…”