Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage

Song, Ji Eun; Park, Seok Ju; Lee, Keun Young; Lee, Wang Jae

doi:10.1080/14767058.2018.1432037

Cited by 11 publications

(9 citation statements)

References 37 publications

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“…They were therefore excluded from the first list of genes primarily linked to cervical insufficiency (n = 12; summarized in Table 2) composed from the data of the literature analysis and additional gene searches. Lastly, only one functional study was conducted to analyze HIF1A gene expression in the amniotic fluid from patients with isolated cervical insufficiency and indicated cerclage [65].…”

Section: Systematic Literature Analysismentioning

confidence: 99%

Genetic landscape of preterm birth due to cervical insufficiency: Comprehensive gene analysis and patient next-generation sequencing data interpretation

et al. 2020

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Preterm delivery is both a traumatizing experience for the patient and a burden on the healthcare system. A condition distinguishable by its phenotype in prematurity is cervical insufficiency, where certain cases exhibit a strong genetic component. Despite genomic advancements, little is known about the genetics of human cervix remodeling during pregnancy. Using selected gene approaches, a few studies have demonstrated an association of common gene variants with cervical insufficiency. However, until now, no study has employed comprehensive methods to investigate this important subject matter. In this study, we asked: i) are there genes reliably linked to cervical insufficiency and, if so, what are their roles? and ii) what is the proportion of cases of non-syndromic cervical insufficiency attributable to these genetic variations? We performed next-generation sequencing on 21 patients with a clinical presentation of cervical insufficiency. To assist the sequencing data interpretation, we retrieved all known genes implicated in cervical functioning through a systematic literature analysis and additional gene searches. These genes were then classified according to their relation to the questions being posed by the study. Patients' sequence variants were filtered for pathogenicity and assigned a likelihood of being contributive to phenotype development. Gene extraction and analysis revealed 12 genes primarily linked to cervical insufficiency, the majority of which are known to cause collagenopathies. Ten patients carried disruptive variants potentially contributive to the development of non-syndromic cervical insufficiency. Pathway enrichment analysis of variant genes from our cohort revealed an increased variation burden in genes playing roles in tissue mechanical and biomechanical properties, i.e. collagen biosynthesis and cell-extracellular matrix communications. Consequently, the proposed idea of cervical insufficiency being a subtle form of collagenopathy, now strengthened by our genetic findings, might open up new opportunities for improved patient evaluation and management.

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Section: Systematic Literature Analysismentioning

confidence: 99%

Genetic landscape of preterm birth due to cervical insufficiency: Comprehensive gene analysis and patient next-generation sequencing data interpretation

et al. 2020

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“…In support of the etiopathological role of inflammatory cytokines in development of PTB, As Sayaril et al [24] reported higher expression of genes for IL1α, IL-1β, IL-6, and TNF-α in plasma of PTB patients than in control pregnant women. Recently, Song et al [25] detected positive correlations between amniotic fluid (AF) hypoxia-inducible factor 1α (HIF1α) and exosomal HIF1α with AF levels of IL-1α, IL-6, and TNF-α in CI patients with physical examination-indicated cerclage and suggested an interaction between AF HIF1α and exosomes and inflammatory cytokines that may induce or contribute the inflammatory cascade in complicated pregnancies.…”

Section: Discussionmentioning

confidence: 99%

Cervical cerclage for Pregnant Women at High-risk of Recurrent Preterm Birth can act beyond its Mechanical Action

Amer

Morsi

Alnoury

2021

Evidence Based Women's Health Journal

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Aim: Evaluation of predictive value of levels of cervicovaginal fluid (CVF) interleukin (IL)-6 and 8, and matrix metalloproteinases (MMP) estimated at time of performing cervical cerclage (CC) in women with history of spontaneous preterm birth (SPTB) for pregnancy duration. Materials and Methods: 59 women had history of SPTB (Study group) and 25 women with no history of SPTB (Control group). Two CVF samples were obtained for ELISA estimation of IL-6, IL-8, MMP8 and MMP9 levels at 14-18 gestational weeks (GW), S1 sampling time, and at time of removal of CC suture, S2 sampling time. CC was applied 4-days after S1 sampling. Results: S1-CVF levels were significantly higher in study versus control women. S2-CVF levels were significantly higher in control, while were significantly lower in study women compared to S1 levels. S2-CVF IL-6 and IL-8 levels were nonsignificantly higher, while levels of MMP were significantly lower in study than control women. Percentages of change in cytokines' levels showed significant differences between study and control groups. Nineteen study women had PTD at <37 GW, while 40 women had labor at >37 GW. Study women had significantly shorter CL and pregnancy duration compared to control women. Pregnancy duration was negatively correlated with percentage of CL change, while positively correlated with percentage of decrease of cytokines levels. Conclusion: CC induced significant decrease of CVF cytokines' levels and allowed prolongation of pregnancy duration for >37 GW in 67.8% of studied women at high risk of SPTB

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“…Moreover, Son et al (17) assayed AF samples of women with CI for 15 cytokines and detected significantly increased levels of most of assayed cytokines compared to control women. Recently, in 2018, Song et al (18) reported significant differences in inflammatory cytokines between women with indicated cerclage and control women.…”

Section: Discussion:-mentioning

confidence: 99%

“…Also, Ito et al (21) reported that rescue CC contributes to prolonged pregnancy and promote neonate survival and was associated with a higher likelihood of reaching at least 28 weeks gestation before delivery in women with bulging fetal membranes. Recently, Song et al (18) reported that when cerclage was indicated on physical examination, AF levels of IL-1α, IL-6 and TNF-α showed significantly negative association with cerclage-to-delivery interval.…”

mentioning

confidence: 99%

A Possible Immuno-Modulatory Action for Cervical Cerclage in Women at Risk for Preterm Labor.

HanyAElKlafM¹,

SaharAMohamedM²

2018

IJAR

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Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage

Cited by 11 publications

References 37 publications

Genetic landscape of preterm birth due to cervical insufficiency: Comprehensive gene analysis and patient next-generation sequencing data interpretation

Genetic landscape of preterm birth due to cervical insufficiency: Comprehensive gene analysis and patient next-generation sequencing data interpretation

Cervical cerclage for Pregnant Women at High-risk of Recurrent Preterm Birth can act beyond its Mechanical Action

A Possible Immuno-Modulatory Action for Cervical Cerclage in Women at Risk for Preterm Labor.

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