Amniotic Fluid Stem Cell Features Supporting Their Putative Role in Fetal Cell Microchimerism

Rosner, Margit; Hengstschläger, Markus

doi:10.1007/978-1-4939-1118-9_5

Cited by 2 publications

(3 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon injection into animals, AFSCs have been demonstrated to be able to integrate into different tissues and beneficially contribute to tissue regeneration [22][23][24][25][26]. These characteristics perfectly match and fulfill the features of PAPCs described above [12,27]. And finally, cffDNA could be released upon apoptosis of defective or excess AFSCs, which are known to immediately undergo programmed cell death upon deregulation of their fine-tuned and strictly balanced survival control [12,28].…”

mentioning

confidence: 76%

Amniotic fluid stem cells and the cell source repertoire for non-invasive prenatal testing

Rosner

Kolbe

Voronin

et al. 2021

Stem Cell Rev and Rep

Self Cite

View full text Add to dashboard Cite

Cell-free fetal DNA (cffDNA)-based non-invasive prenatal testing (NIPT) is considered to be a very promising screening tool for pregnant women with an increased risk of fetal aneuploidy. Already millions of women worldwide underwent NIPT. However, due to the observed false-positive and false-negative results, this screening approach does not fulfil the criteria of a diagnostic test. Accordingly, positive results still require risk-carrying invasive prenatal testing, such as amniocentesis or chorionic villus sampling (CVS), for confirmation. Such hurdles need to be overcome before NIPT could become a diagnostic approach widely used in the general population. Here we discuss new evidence that besides the placenta amniotic fluid stem cells (AFSCs) could also represent an origin of cffDNA in the mother’s blood. A comprehensive picture of the involved cell source repertoire could pave the way to more reliable interpretations of NIPT results and ameliorate counselling of advice-seeking patients. Graphical abstract

show abstract

mentioning

confidence: 76%

Amniotic fluid stem cells and the cell source repertoire for non-invasive prenatal testing

Rosner

Kolbe

Voronin

et al. 2021

Stem Cell Rev and Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…In earlier publications we have highlighted the fact that the currently known spectrum of properties of hAFSCs perfectly matches features which must be designated to a stem cell type to be considered as a candidate for the origin of pregnancy‐associated progenitor cells (PAPCs). 76 , 77 Together with cell‐free fetal DNA (cffDNA) in the mother's circulation, PAPCs in the tissue of pregnant women, constitute the well‐known phenomenon of fetal cell microchimerism (FCM). Because of their plasticity of morphology and diverse phenotypes, PAPCs are consensually considered to be stem cells, with characteristics and potentials between those of pluripotent ESCs and adult stem cells.…”

Section: Discussionmentioning

confidence: 99%

“…But we also argued, that other features, which are indispensable to fulfill the required spectrum of properties of PAPCs, including mainly 3D migration and extravasation potentials, have not been proven for hAFSCs so far. 77 PAPCs are known to enter the maternal peripheral blood system, distribute, exit the vasculature and infiltrate maternal tissues and organs. 78 , 79 , 80 , 81 , 82 In this respect, the here proven invasion and transendothelial migration capacities of hAFSCs represent the missing pieces to complete this puzzle in support of their suggested role in FCM.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Migration of Human Amniotic Fluid Stem Cells Involves Mesenchymal and Amoeboid Modes and is Regulated by mTORC1

Rosner

Hengstschläger

2021

Stem Cells

Self Cite

View full text Add to dashboard Cite

Three-dimensional (3D) cell migration is an integral part of many physiologic processes. Although being well studied in the context of adult tissue homeostasis and cancer development, remarkably little is known about the invasive behavior of human stem cells. Using two different kinds of invasion assays, this study aimed at investigating and characterizing the 3D migratory capacity of human amniotic fluid stem cells (hAFSCs), a well-established fetal stem cell type. Eight hAFSC lines were found to harbor pronounced potential to penetrate basement membrane (BM)-like matrices. Morphological examination and inhibitor approaches revealed that 3D migration of hAFSCs involves both the matrix metalloprotease-dependent mesenchymal, elongated mode and the Rho-associated protein kinase-dependent amoeboid, round mode. Moreover, hAFSCs could be shown to harbor transendothelial migration capacity and to exhibit a motility-associated marker expression pattern. Finally, the potential to cross extracellular matrix was found to be induced by mTORC1-activating growth factors and reduced by blocking mTORC1 activity. Taken together, this report provides the first demonstration that human stem cells exhibit mTORC1-dependent invasive capacity and can concurrently make use of mesenchymal and amoeboid 3D cell migration modes, which represents an important step toward the full biological characterization of fetal human stem cells with relevance to both developmental research and stem cell-based therapy.

show abstract

Amniotic Fluid Stem Cell Features Supporting Their Putative Role in Fetal Cell Microchimerism

Cited by 2 publications

References 73 publications

Amniotic fluid stem cells and the cell source repertoire for non-invasive prenatal testing

Amniotic fluid stem cells and the cell source repertoire for non-invasive prenatal testing

Three-Dimensional Migration of Human Amniotic Fluid Stem Cells Involves Mesenchymal and Amoeboid Modes and is Regulated by mTORC1

Contact Info

Product

Resources

About