Amniotic Membrane Transplantation in Strabismus Surgery

Kassem, Rehab R.; El-Mofty, Randa Mohamed Abdel-Moneim

doi:10.1080/02713683.2018.1562555

Cited by 14 publications

(8 citation statements)

References 64 publications

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“…78,80,82 There is a growing body of evidence, evaluating the use of AMs in strabismus surgery. [83][84][85][86][87][88][89] Similar to the present study, a 2019 review article evaluated the use of AMs with different processing methodologies for extraocular muscle surgery. 89 The authors concluded that dried AM does not effectively limit adhesion formation.…”

Section: Ddham Supports An Initial Inflammatory Response Followed By ...mentioning

confidence: 90%

“…[83][84][85][86][87][88][89] Similar to the present study, a 2019 review article evaluated the use of AMs with different processing methodologies for extraocular muscle surgery. 89 The authors concluded that dried AM does not effectively limit adhesion formation. 89 This conclusion, however, is based on the results from three animal studies 84,85,87 and two case reports.…”

Section: Ddham Supports An Initial Inflammatory Response Followed By ...mentioning

confidence: 90%

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An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study

Mao

Protzman²,

John

et al. 2022

J Biomed Mater Res

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Amniotic membrane (AM) is a naturally derived biomaterial with biological and mechanical properties important to Ophthalmology. The epithelial side of the AM promotes epithelialization, while the stromal side regulates inflammation. However, not all AMs are equal. AMs undergo different processing with resultant changes in cellular content and structure. This study evaluates the effects of sidedness and processing on human corneal epithelial cell (HCEC) activity, the effect of processing on HCEC inflammatory response, and then a case study is presented. Three differently processed, commercially available ocular AMs were selected: (1) Biovance ® 3L Ocular, a decellularized, dehydrated human AM (DDHAM), (2) AMBIO2 ® , a dehydrated human AM (DHAM), and(3) AmnioGraft ® , a cryopreserved human AM (CHAM). HCECs were seeded onto the AMs and incubated for 1, 4 and 7 days. Cell adhesion and viability were evaluated using alamarBlue assay. HCEC migration was evaluated using a scratch wound assay. An inflammatory response was induced by TNF-α treatment. The effect of AM on the expression of pro-inflammatory genes in HCECs was compared using quantitative polymerase chain reaction (qPCR). Staining confirmed complete decellularization and the absence of nuclei in DDHAM. HCEC activity was best supported on the stromal side of DDHAM. Under inflammatory stimulation, DDHAM promoted a higher initial inflammatory response with a declining trend across time. Clinically, DDHAM was used to successfully treat anterior basement membrane dystrophy. Compared with DHAM and CHAM, DDHAM had significant positive effects on the cellular activities of HCECs in vitro, which may suggest greater ocular cell compatibility in vivo.

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Section: Ddham Supports An Initial Inflammatory Response Followed By ...mentioning

confidence: 90%

Section: Ddham Supports An Initial Inflammatory Response Followed By ...mentioning

confidence: 90%

An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study

Mao

Protzman²,

John

et al. 2022

J Biomed Mater Res

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“…The loss of lubricin may explain why dried, but not cryopreserved, AM loses its anti-adhesive, antifibrotic and anti-inflammatory abilities that typically combine to inhibit scar formation. [19][20][21][22][23] Indeed, this absence of lubricin may account for why the use of dried AM was unable to suppress inflammation, reduce adhesion generation, prevent scar formation and decrease the risk of symblepharon development after strabismus surgery. 20,22,24 How would the loss of lubricin in FD-AMs result in such sequelae?…”

Section: Discussionmentioning

confidence: 99%

Expression of Lubricin in the Human Amniotic Membrane

Wang

Chen

Sullivan

et al. 2019

Cornea

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Purpose-Lubricin, a boundary lubricant, is the body's unique anti-adhesive, anti-fibrotic, antifriction and anti-inflammatory glycoprotein. This amphiphile is produced by numerous tissues and acts to regulate a number of processes, such as homeostasis, shear stress, tissue development, innate immunity, inflammation and wound healing. We hypothesize that lubricin is also synthesized and expressed by the amniotic membrane (AM), which also possesses anti-adhesive, anti-fibrotic and anti-inflammatory properties. We also hypothesize that lubricin, at least in part, mediates these AM capabilities. Our goal was to test our hypothesis.Methods-We obtained multiple samples of fresh, cryopreserved (CP) and freeze-dried (FD) human AMs, as well as fresh placental tissue positive controls, and processed them for light microscopy, immunofluorescence and Western blot analyses. We also evaluated the ability of recombinant human (rh)-lubricin to associate with FD-AM.Results-Our results demonstrate that all fresh placental, fresh AM and CP-AM samples contained lubricin. Lubricin was expressed in placental chorionic villi, AM epithelial and stromal cells, and CP-AM epithelia. No lubricin could be detected in FD-AMs, but could be restored in FD-AM after overnight incubation with rh-lubricin.Conclusions-This study supports our hypothesis that lubricin is expressed in human AMs. In addition, our data show that preservation methods influence the extent of this expression. Indeed, the disappearance of lubricin in FD-AMs may explain why dried AM reportedly loses its antiinflammatory and anti-scarring abilities. It is possible that lubricin may mediate, at least in part, many of the biological properties of AMs.

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“…Since then, there have been many applications for ophthalmic disease ranging from ocular burn, corneal defects, retinal problems, strabismus, and neoplasia. However, most of them used human amnion [54][55][56][57][58][59][60].…”

Section: Ophthalmic Surgerymentioning

confidence: 99%

Future of Bovine Amniotic Membrane: Bovine Membrane Application on Wound Healing, Surgery and Prospect of Use for Urethral Reconstruction

Hariastawa¹,

Sutantio²

2022

Bovine Science - Challenges and Advances

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This chapter describes how bovine amniotic membrane could be indicated for wound healing, especially in complex surgery such as urethral reconstruction. Chemical studies have assessed both histologically and immunohistochemically that bovine amniotic membrane creates scaffold for wound healing. Whereas, clinical studies have shown that bovine amniotic membrane property could be substituted for wound dressing hence improving skin or mucosal integrity. Bovine membrane has been known to be used for many specialties such as ocular surgery, neurosurgery, maxillofacial and orthopedic surgery. This chapter includes such studies and shows the usage possibility of bovine amniotic membrane for other complex defect as shown in urethral reconstruction.

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Amniotic Membrane Transplantation in Strabismus Surgery

Cited by 14 publications

References 64 publications

An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study

An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study

Expression of Lubricin in the Human Amniotic Membrane

Future of Bovine Amniotic Membrane: Bovine Membrane Application on Wound Healing, Surgery and Prospect of Use for Urethral Reconstruction

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