Amniotic membranes in ophthalmology: long term data on transplantation outcomes

Paolin, Adolfo; Cogliati, Elisa; Trojan, Diletta; Griffoni, Carlo; Grassetto, Andrea; Elbadawy, Hossein M.; Ponzin, Diego

doi:10.1007/s10561-015-9520-y

Cited by 61 publications

(52 citation statements)

References 27 publications

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“…Requests have been received and completed from 58 ophthalmic surgeons across all states and territories except Northern Territory suggesting a basic national demand for AM. Fifty‐nine percent of recipients were male with a mean age of 64.0 ± 17.3 years (range 6 to 93) corresponding with population findings from the existing ophthalmic literature . Seven patients required multiple tissue applications for surface reconstruction or tectonic repair (neoplasia, corneal ulcer at risk of perforation, infectious scleritis and alkali burn).…”

Section: Literature Comparison: Main Indications For Use Of Amnion Timentioning

confidence: 84%

“…The leading indication in Mexico and Italian populations is pterygium and corneal ulcer repair, respectively. The incidence of ocular surface neoplasia has been shown to be significantly greater in the southern hemisphere which may have contributed to the increased representation in the local population by comparison . Our sample remains small however and the increased awareness and use of the AM tissue utility is likely to see the list evolve further.…”

Section: Literature Comparison: Main Indications For Use Of Amnion Timentioning

confidence: 92%

See 1 more Smart Citation

Ophthalmic use of amniotic membrane tissue in Australia: Introduction and initial use of a service

Treloggen

McKeon

Hodge

et al. 2019

Clinical Exper Ophthalmology

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Section: Literature Comparison: Main Indications For Use Of Amnion Timentioning

confidence: 84%

Section: Literature Comparison: Main Indications For Use Of Amnion Timentioning

confidence: 92%

Ophthalmic use of amniotic membrane tissue in Australia: Introduction and initial use of a service

Treloggen

McKeon

Hodge

et al. 2019

Clinical Exper Ophthalmology

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“…The reduction in the proliferation of HPDLFs can also be due to foetal hyaluronic acid, which is one of the components of HAM. Foetal hyaluronic acid suppresses the transforming growth factor-beta (TGF-β) signalling by reducing the expression of TGF β-1, β-2 and β-3 isoforms and the expression of the TGF receptor, which has an inhibiting effect on the proliferation of fibroblasts (Lee et al 2000;Paolin et al 2016). This is also the main mechanism behind its anti-scarring properties (Tseng et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Human Amniotic Membrane as a Scaffold for Periodontal Ligament Fibroblast Attachment and Proliferation

Elahi¹,

Taib²,

Berahim³

et al. 2019

JSM

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This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue regeneration such as low immunogenicity, anti-fibrosis, anti-inflammation, and a rich extracellular matrix component. Commercially available HPDLFs were seeded onto the HAM, and the attachment and proliferation of the cells were observed through scanning electron microscopy (SEM) and histological analysis. Cell viability was assessed using the alamarBlue® proliferation assay at days 1, 3, 7, 14 and 21. Histologically, the HPDLFs showed a monolayer to multilayer attachment onto the HAM from day 1 to day 7. The SEM analysis demonstrated that the HPDLFs had attached appropriately onto the HAM surface at day 1 to day 3, and began overlapping at day 7, while maintaining their flat shape. However, by days 14 and 21, there was an alteration in the morphology of the cells, where they later became rounded. The proliferation assay showed that the viability of the HPDLFs on the HAM had increased significantly from day 1 to day 7 (p=0.012), but later showed significant reduction at day 14 (p=0.002) and day 21 (p=0.005). In conclusion, this study showed that the HAM was able to function well as a scaffold for HPDLFs within 7 days, and thus, it can be a promising scaffold for periodontal regeneration. However, the behaviour of the cells in relation to the membrane over longer culture duration warrants further investigation. ABSTRAK Kajian ini bertujuan untuk menilai keupayaan membran amniotik manusia (HAM) untuk bertindak sebagai perancah bagi pertumbuhan sel-sel utama dalam regenerasi periodontal iaitu sel fibroblas ligamen periodontal manusia (HPDLFs). HAM mempunyai banyak ciri biologi yang sesuai untuk pertumbuhan semula tisu periodontal seperti keimunogenan yang rendah, anti-fibrosis, anti-keradangan dan kaya dengan komponen matriks luar sel. HPDLFs yang tersedia secara komersial telah dibiakkan ke atas HAM, dan pelekatan serta pengembangbiakan HPDLFs diperhatikan melalui pemeriksaan mikroskop imbasan elektron (SEM) dan analisis histologi. Daya tahan sel telah dinilai menggunakan ujian percambahan alamarBlue® pada hari 1, 3, 7, 14 dan 21. Secara histologi, HPDLFs menunjukkan pelekatan ekalapisan kepada beberapa lapisan ke atas HAM daripada hari pertama hingga ke-7. Analisis SEM menunjukkan bahawa HPDLFs telah melekat dengan baik pada permukaan HAM pada hari pertama hingga hari ke-3 dan mula bertindih pada hari ke-7, sambil mengekalkan bentuknya yang rata. Bagaimanapun, pada hari ke-14 dan ke-21, terdapat perubahan pada morfologi sel, dengan mereka kemudiannya menjadi bulat. Ujian percambahan menunjukkan bahawa daya tahan sel HPDLFs pada HAM meningkat dengan ketara daripada hari pertama hingga hari ke-7 (p=0.012) namun kemudiannya menunjukkan penurunan yang ketara pada hari ke-14 (p=0.002) dan hari ke-21 (p=0.005). Kesimpulannya, kaj...

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“…In addition, tissue remodeling was reported with their use via stimulating apoptosis of T lymphocytes and regulating activated macrophages. Paolini et al, (2016) also revealed that AM acts as a scaffold for proliferation and differentiation due to its content of fibronectin, elastin, hyaluronic acid and collagen type I, III, IV, V and VI [41] . Also, various growth factors have been detected in cryopreserved AMs including epidermal growth factor and transforming growth factors which play a crucial role in the anti-scarring and anti-inflammatory effects of AM, in addition to enhancing re-epithelialization and healing [42] .…”

Section: Discussionmentioning

confidence: 99%

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

Ebrahim

Mohammed

Dessouky

et al. 2017

Egyptian Journal of Histology

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Background: Corneal alkali burns are common ophthalmic emergencies which may lead to permanent visual impairment. In spite of great advances made in the treatment of these cases, the structural and functional restoration of corneal alkali burns remains challenging. Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been demonstrated to enhance corneal healing. However the problem remains in finding a suitable vehicle to deliver BM-MSCs to the site of injury. Objectives: To evaluate the therapeutic potential of BM-MSCs loaded on two biological vehicles (amniotic membrane versus platelet-rich plasma gel) in corneal alkali burns. Materials & Methods: Fifty rats were randomly divided into five equal groups. Group I (control group), group II (corneal ulcer group) and group III (recovery group). Group IV rats with corneal ulcers were treated with amniotic membrane loaded with BM-MSCs while in the group V the rats with corneal ulcers were treated with platelet-rich plasma cells loaded with BMMSCs. The corneal specimens were processed for the light microscope, transmission electron microscope examination, and RT qPCR assay for VEGF and iNOS. Results: Group II showed epithelial separation and denudation with disorganized stroma and disrupted Descemet's membrane and endothelium. Group III showed residual epithelial separation and vacuolation with neovascularization of the disorganized stroma. Group IV showed thinning of corneal epithelium with inflammatory infiltration of the stroma. Group V corneas revealed restoration of corneal epithelial thickness, organized stroma and continuous Descemet's membrane. Also, there was an upregulation of iNOS & VEGF gene expression in groups II and III which was downregulated in group IV and V. Conclusions: The combination of BM-MSCs with PRP gel was a promising treatment for corneal alkali burns and may be applicable for other types of corneal disorders.

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Amniotic membranes in ophthalmology: long term data on transplantation outcomes

Cited by 61 publications

References 27 publications

Ophthalmic use of amniotic membrane tissue in Australia: Introduction and initial use of a service

Ophthalmic use of amniotic membrane tissue in Australia: Introduction and initial use of a service

Evaluation of the Human Amniotic Membrane as a Scaffold for Periodontal Ligament Fibroblast Attachment and Proliferation

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

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