Amoebicidal Effects of Contact Lens Disinfecting Solutions

Abstract

The Vi-Cell analyzer offers a simple and effective method of determining amoebicidal activity. Our results show that the CL solutions tested could not satisfactorily kill Acanthamoeba.

Contact lens care solution killing efficacy against Acanthamoeba castellanii by in vitro testing and live-imaging

“…A number of published studies have investigated the effects of a range of contact lens care solutions against Acanthamoeba, however the results have varied considerably with inconsistency likely related to multiple factors including lack of standard methodology, assay technique, strain, cyst maturity, inoculum preparation [21,23,25,28,[33][34][35][36]. In our study the maximum percent killing of trophozoites by a contact lens care solution (polyquaternium/alexidine-based) was 73%.…”

Effects of multipurpose solutions on the adhesion of Acanthamoeba to rigid gas permeable contact lenses

“…The clinical concentration range of PHMB varies from 0.00001% to 0.0005%, and all three MPSs tested had same PHMB concentration of 0.0005% with different reduction effects on AC trophozoites adhesion to RGP lenses. Although there is no evidence what concentration of PHMB in MPS is required to be effective against AC trophozoites until now, several studies have suggested that the currently available MPS including PHMB could not satisfactorily kill Acanthamoeba and was insufficient for the protective effect against Acanthamoeba . In addition, 0.02% PHMB has been reported to show therapeutic efficacy for the treatment of Acanthamoeba keratitis .…”

Broad Spectrum Antimicrobial Activity of Melimine Covalently Bound to Contact Lenses

“…29 Many commonly used contact lens disinfecting solutions have only limited amoebicidal efficacy. 66 The recent outbreak of contact lens related Acanthamoeba keratitis associated with use of Complete MoisturePlus contact lens disinfecting solution 10 and persistent elevated numbers of events, even after removal of this solution from sale, 10,28 clearly indicates a need for an effective strategy to help reduce the incidence of this disease. In this study, for the first time, an antimicrobial peptide attached to contact lens surface was shown to have amoebicidal activity.…”