<i>In Vitro</i> Efficacy of <i>Crataegus oxycantha</i> L. (Hawthorn) and Its Major Components against ATCC and Clinical Strains of <i>Ureaplasma urealyticum</i>

Abstract

Crataegus oxycantha L., commonly known as hawthorn, has traditionally been used for its beneficial effect on cardiovascular health, which is related to its flavonoid content. The aim of the present study was to evaluate the antibacterial properties of a fluid extract and a hydro-ethanolic macerate from buds of Crataegus oxycantha against clinical isolates of Ureaplasma urealyticum. The major purified flavonoids present in the extracts were also tested against ATCC strains and clinical isolates. Both the fluid extract and the hydro-ethanolic macerate were active against thirty-due clinical strains of U. urealyticum, with MIC ranges between 15.6 and 250 μg/ml and 15.6 and 62.5 μg/ml, respectively. All pure organic compounds, with the exception of rutin, showed activity against the strains tested, luteolin 3,7-diglucoside being the most active compound (MICs in the range of 0.48 and 1.95 μg/ml), followed by apigenin-7-O-glucoside (MICs in the range of 0.48 and 3.9 μg/ml). The activity of the pure flavonoids was greater against the clinical isolates compared to the ATCC strains. The data presented here demonstrate that flavonoids present in Crataegus oxycantha are effective against clinical isolates of U. urealyticum and could be used in combination with antibiotics in order to combat resistance.

Phytochemical composition and bioactivities of hawthorn ( Crataegus spp.): review of recent research advances

“…C. oxycantha buds fluid extract and the hydro-ethanolic macerate were active against thirty-due clinical strains of U. urealyticum, with MIC ranges of 15.6-250 and 15.6-62.5 μg/mL, respectively. Meanwhile, among the major purified flavonoids luteolin 3,7-diglucoside and apigenin-7-O-glucoside were the most active compounds with MICs 0.48-1.95 and 0.48-3.9 μg/mL, respectively (Bisignano et al, 2016). Ethanolic extract of C. azarolus var.…”

Polyphenolic Compounds of Crataegus Berry, Leaf, and Flower Extracts Affect Viability and Invasive Potential of Human Glioblastoma Cells

“…Today, Crataegus berries and flowers are officially recognized as herbal raw materials and have been included in European, American, and Canadian pharmacopeias. Recent research confirmed and extended the possibilities of the traditional use of this plant, among others, to anti-inflammatory [ 8 , 9 ], anti-oxidant [ 10 , 11 ], anti-aging [ 12 ], anti-depressant [ 13 ], anti-viral, anti-fungal, anti-bacterial [ 14 ], hypoglycemic, hepatoprotective [ 15 ], gastroprotective [ 16 ], immunostimulatory, hypolipidemic, hypocholesterolemic, and anti-atherosclerotic effects [ 17 ]. Furthermore, Crataegus extracts are considered as putative therapeutic agents for the treatment of hepatocellular carcinoma, breast cancer, melanoma, and colorectal cancer [ 18 , 19 , 20 ].…”

Hawthorn (Crataegus spp.): An Updated Overview on Its Beneficial Properties

“…Many in vitro studies reported different health-promoting effects for hawthorn extracts [89][90][91][92]. The administration of homogeneous polysaccharide (HPS) extracted from hawthorn at a concentration of 125-1000 µg/mL showed anticancer activity against a human colon cancer cell line HCT116, after 12 h by arresting the cell cycle and inducing cell apoptosis through extrinsic and intrinsic mechanisms using P38 mitogen-activated protein kinase and the phosphatidylinositol-3-kinase/AKT/mammalian target of rapamycin signaling pathway [93].…”