<i>In Vitro</i> Studies of NIPAAM-MAA-VP Copolymer-Coated Magnetic Nanoparticles for Controlled Anticancer Drug Release*

Abstract

Thermosensetive poly(N-isopropylacrylamide)-based magnetic nanoparticles were synthesized by free radical polymerization of N-isopropylacrylamide (NIPPAMs), methacrylic acid (MAA), and vinyl pyrrolidone (VP) in the presence of methylene-bis-acrylamide as cross linking agent. The Fe 3 O 4 magnetic nanoparticls were prepared by chemical precipitation of Fe salts in the ratio of 1:2 under alkaline and inert condition. Thermosensitive crosslinked P (NI-PAAM-MAA-VP) copolymers were characterized by FT-IR and H-NMR. The pH and thermosensitive copolymer was used for preparation of drug loaded magnetic nanoparticles, and doxorubicin (DOX) was used as a typical anticancer drug. The amount of the loaded drug and drug release amount were determined by UV measurements. Scanning electron microscopy (SEM) and lower critical solution temperature (LCST) were used to determine the particle surface morphology and the phase transition temperature of the nanoparticles respectively. The release behavior of DOX at pH = 7.4 and 37˚C was studied. The result indicated that this thermosensetive magnetic nanoparticle has a high drug loading capacity and favorable linear release property for DOX without initial burst release. Thus this system is promising for the application in targeted smart anticancer drug delivery.

Constrained thermoresponsive polymers – new insights into fundamentals and applications

“…PNIPAAm-preparation/analysis [186] PDMAAm@Fe 3 O 4 preparation/analysis [187] PNIPAAm-PNHMA@ Fe 3 O 4 drug release, preparation [188][189][190][191] PNIPAAm@SiO 2 molecule delivery, preparation [191][192][193][194] PNIPAAm@Fe 3 O 4 microfluidic separation and assay; sensing; drug release, preparation [195][196][197][198][199][200][201] PNIPAAm@Au fundamental investigation, preparation, cell up-take control [200,202] PNIPAAm-PAm@Au PNIPAAm-PNVP@Fe 3 O 4 cell up-take control drug delivery/cell separation, preparation [203] PNIPAAm-PDMAEMA@Fe 3 O 4 /Au catalysis, preparation [204] PNIPAAm-PAA@UCNP sensing, preparation [205] PNIPAAm-PMAA-PVP@Fe 2 O 3 drug delivery, preparation [206] POX-based grafted nanoparticles [207] PIPOX@Fe 3 O 4 preparation/analysis [208] PIPOX-PEtOX@SPION cell up-take control [209] PEtOX/PPropOX-PVIm/P4VP@Ag preparation/analysis [210] PEG or PEG derivates-based grafted nanoparticles [211] PEG-PDMAEMA@Au preparation/analysis [212] PEGMA@Au preparation/analysis [151,213] PMEO…”

Comparison, synthesis and evaluation of anticancer drug-loaded polymeric nanoparticles on breast cancer cell lines

“…To achieve precipitation, after 1 h, ice-cold diethyl ether was added under stirring conditions , Fallahzadeh et al 2010, Ebrahimnezhad et al 2013, Pourhassan-Moghaddam et al 2013, Ahmadi et al 2014, Ghasemali et al 2013). The solution was precipitated after 24 h. The precipitate was stored in a desiccator until it was used ( Davaran et al 2013, Ghasemali et al 2013. The morphology of the nanoparticles was examined using SEM.…”

Immunology and vaccines and nanovaccines forHelicobacter pyloriinfection

“…Such a material is so small that it can simply enter the cell; consequently, nanomaterials can be used in vivo or in vitro for biological applications. This concept has been found to be constructive in developing nanovaccines for delivery through different routes of administration: oral, nasal and intradermal route [63][64][65][66][67].…”