<i>In Vivo</i> and <i>in Vitro</i> Evaluation of Anti Diabetic and Insulin Secretagogue Activities of <i>Capparis zeylanica</i>

Abstract

Since ancient times, traditional medicines have been in the usage for the treatment of Diabetes mellitus. An edible fruit from traditional medicinal plant Capparis zeylanica (CZ) was studied for its anti diabetic, insulin secretagogue activities and mechanisms involved in it. In Streptozotocin induced diabetes rats, oral administration of Capparis zeylanica methanolic extract (CZME) (200 mg/kg body weight) for 28 days showed a significant reduction in blood glucose levels by 35.53% and enhanced circulating insulin levels by 81.82% than the diabetic control rats. The insulin secretagogue activity mechanisms of the extract were evaluated by using mouse insulinoma beta cell line (MIN6-β). The extract stimulated insulin release in dependent manner of glucose concentration (3-16.7 mM) and extract dose (5-500 μg/mL). The insulin releasing effect of the extract was significantly enhanced by 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium and K + depolarized media. This insulin release was significantly reduced in calcium blocking conditions (by nifedipine and EGTA), in the presence of potassium channel opener (diazoxide). Hence, anti diabetic activity of CZME might be a result of its stimulatory effect on insulin release from pancreatic beta cells via K ATP channel dependent and independent ways. These results indicate that CZ fruits have the potential to use in diabetes therapy.

Cardioprotective effects of Ferulic acid in Streptozotocin- induced diabetic rats

“…Research Laboratories (Mumbai, India). [15] and glibenclamide at a dose of 5 mg/kg body weight [16] respectively (N+FA and N+G). The daily intragastric treatments using the fresh suspension of FA and glibenclamide were continued for 60 days.…”

Bioaccessibility and bioavailability of soluble polyphenols from Capparis zeylanica fruit according to drying method

“…These fruits are consumed by tribal population of different tribe viz., Medara, Budabudikae, Lambani, Korava, and adavichencharu of southern India [1]. Methanolic extracts of C. zeylanica fruits were reported for their anti-diabetic activity in insulinoma beta cells (MIN6-β) and Streptozotocin-induced diabetic rat model [2].…”

Insulin Secretagogue Effect of Roots of Ravenala Madagascariensis Sonn. - An in Vitro Study

“…The mechanism of antidiabetic activity of the most drugs of natural origin suggests the stimulation of insulin release from pancreatic beta-cells [6]. In the reported literature, various plant extracts including Capparis zeylanica [6], Gymnema sylvestre [7], Caulerpa lentillifera [8], Gynura procumbens [9], Ficus deltoidea [3], and Abutilon indicum [4] were explored for their insulin secretagogue activity in beta-cell lines.…”