Ampelography - An old technique with future uses: the case of minor varieties of <em>Vitis vinifera</em> L. from the Balearic Islands

Abstract

&lt;p style="text-align: justify;"&gt;&lt;strong&gt;Aims&lt;/strong&gt;: We report the genetic, phenological, agronomic and ampelographic characterization of 27 minor grapevine accessions (&lt;em&gt;Vitis vinifera &lt;/em&gt;L.) from the Balearic Islands (Spain). The influence of occasional climatic phenomena (hailstorm) and the ampelographer’s experience on these characteristics was studied.&lt;/p&gt;&lt;p style="text-align: justify;"&gt;&lt;strong&gt;Methods and results&lt;/strong&gt;: In 2006 and 2007, grapevine accessions were analyzed using 57 OIV (Office International de la Vigne et du Vin) qualitative and quantitative descriptors and six microsatellite loci (SSR). Ampelography is a good preliminary technique for the identification of plant material, since the ampelographic description carried out showed a high degree of similarity between the repetitions of one single accession (higher than 90%), confirming the microsatellite results. The colour of the young leaf’s upper side (OIV-051), the juiciness of the flesh (OIV-232) and the firmness of berry flesh (OIV-235) were the most difficult characters to distinguish by ampelographers. In spite of the greater similarity found among the varieties studied, there were a few strong discriminant characters (OIV-225, OIV-084, OIV-053, and OIV-004). In addition, the ampelographic descriptions, agronomic parameters and phenology were influenced by hailstorm.&lt;/p&gt;&lt;p style="text-align: justify;"&gt;&lt;strong&gt;Conclusions&lt;/strong&gt;: The combination of morphological and molecular characterizations of 27 accessions collected in the Balearic Islands (Spain) allowed their classification into 17 different vine varieties. The genetic analysis identified Beba blanca as a possible somatic mutant derived from Beba roja. The hailstorm increased the vegetative period and mostly affected mature leaves, bunches, agronomic characteristics and must composition.&lt;/p&gt;&lt;p style="text-align: justify;"&gt;&lt;strong&gt;Significance and impact of the study&lt;/strong&gt;: The present work characterizes, for the first time, the ampelographic and molecular profiles of these minor varieties. It is provide agronomic information about old cultivars never published before which could be interesting for the grape growers.&lt;/p&gt;

Ex situ ampelographical characterisation of wildVitis viniferafrom fifty-one Spanish populations

“…The Numerical Taxonomy System [NTSYS v2.1; Rholf ()] was used for similarity analysis and correspondence analysis. Hierarchical cluster analysis was carried out using the correlation coefficient (Cervera et al ) and the unweighted pair group method analysis as a linkage method (García Muñoz et al ).…”

“…Most historical descriptions of grape cultivars were based on morphological methods (Herrera , Clemente , Galet ). Nowadays, ampelography is a preliminary method for clarifying the identity of plant material (García Muñoz et al ). It is also the method used by different countries to include a cultivar in official lists.…”

“…It is also the method used by different countries to include a cultivar in official lists. The use of morphological descriptors plus molecular techniques, however, is highly recommended to characterise Vitis genetic material to obtain an accurate identification (García Muñoz et al ). For this reason, this study is based on previous molecular studies (De Andrés et al ).…”

“…Most morphological studies on wild vines have been performed in situ and under different environmental conditions. Prospecting and studying the material ex situ under the same growing conditions, however, has recently been suggested (Di Vecchi , García Muñoz et al ). Thus, the ampelographical descriptions in this study were carried out ex situ in the same location to avoid the possible influence of environmental factors.…”

Genetic relationships of some local and introduced grapes (Vitis viniferaL.) by microsatellite markers

“…The conventional methods of identification rely on ampelography based on the morphological differences between the varieties [11][12][13], i.e. leaf shape and contours, growing shoots characteristics, flower sex, grape cluster shape and grape colour.…”