Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes

Villalonga, Anabel; Estabiel, Itziar; Pérez-Calabuig, Ana M.; Mayol, Beatriz; Parrado, Concepción; Villalonga, Reynaldo

doi:10.1016/j.bios.2021.113203

Cited by 34 publications

(12 citation statements)

References 36 publications

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“…Inspired by comprehensive application of HRP in ELISA, we initiated to explore the application of HRP activity based fluorescence turn-on sensing system in the HRP-labeled immunoassay target antigen. The cTnI, the biomarker of myocardial infarction (AMI), was adopted as our target antigen ( Kergaravet et al, 2012 ; Yuan et al, 2012 ; Anabel et al, 2021 ; Carlo et al, 2021). The mouse anti-cTnI monoclonal antibody, goat anti-cTnI antibody and HRP-conjugated rabbit anti-goat IgG were employed as the capture antibody, primary antibody and secondary antibody, respectively.…”

Section: Resultsmentioning

confidence: 99%

Horseradish peroxidase-triggered direct in situ fluorescent immunoassay platform for sensing cardiac troponin I and SARS-CoV-2 nucleocapsid protein in serum

Liu

Ruan

et al. 2022

Biosensors and Bioelectronics

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Section: Resultsmentioning

confidence: 99%

Horseradish peroxidase-triggered direct in situ fluorescent immunoassay platform for sensing cardiac troponin I and SARS-CoV-2 nucleocapsid protein in serum

Liu

Ruan

et al. 2022

Biosensors and Bioelectronics

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“…It should be noted that an amperometric sensor has also been used to detect cardiac troponin I, using carbon electrodes coated with a graphene oxide derivative as a switching element [44]. This carboxylic acid enriched nanomaterial allowed the easy and high immobilization of the capture molecules on the electrode surface.…”

Section: Capacitive Biosensorsmentioning

confidence: 99%

A Review on Cardiac Biomarkers Detection for Heart Failure Prognosis

Angeliki¹,

Georgas²,

Hristoforou³

2022

JICOA

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Nowadays, problems in healthcare sector seem to be more crucial than ever. Among these, cardiovascular diseases (CVDs) play an important role, being for years a major threat to human health. In a bid to reduce the number of deaths due to heart diseases by taking precautions, science has turned to the development of devices able to offer early diagnosis of a heart failure. Recently, many biosensors have been developed based on cardiac biomarkers that can be detected in blood and predict the risk of heart failure. Of these, cardiac troponin is the best known, while C-reactive protein and myoglobin have also been used. Troponin is a complex of proteins found in the thin filaments of striated muscles and consists of three protein subunits, I, C and T. In case of myocardial malfunction, troponin is released into the blood. Consequently, the detection of elevated troponin levels at an early stage in human serum, is an indication of high risk of cardiovascular event enabling early medical intervention and treatment. In the present work, after the analysis of heart diseases, medical tests, troponin and its role in controlling myocardial health, there is an overview of the available biosensor platforms for cardiac biomarkers detection.

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“…However, most of these methods involve costly equipment and complicated sample processing and suffer from several drawbacks such as a low efficiency and/or false-positive results, which greatly restrict their widespread use [ 14 , 15 , 16 ]. Alternatively, electrochemical sensors have drawn increasing attention in detecting mycotoxins, owing to their high efficiency and simplicity [ 15 , 17 , 18 , 19 ]. For instance, Shi et al developed a novel electrochemical immunosensor for AFB 1 detection based on Au nanoparticles supported by graphene, which showed a limit of detection (LOD) of 0.001 ng mL −1 [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1

et al. 2022

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High-performance electrochemical biosensors for the rapid detection of aflatoxin B1 (AFB1) are urgently required in the food industry. Herein, a multi-scaled electrochemical biosensor was fabricated by assembling carboxylated polystyrene nanospheres, an aptamer and horseradish peroxidase into a free-standing carbon nanofiber/carbon felt support. The resulting electrochemical biosensor possessed an exceptional performance, owing to the unique structures as well as the synergistic effects of the components. The 3D porous carbon nanofiber/carbon felt support served as an ideal substrate, owing to the excellent conductivity and facile diffusion of the reactants. The integration of carboxylated polystyrene nanospheres with horseradish peroxidase was employed as a signal amplification probe to enhance the electrochemical responses via catalyzing the decomposition of hydrogen peroxide. With the aid of the aptamer, the prepared sensors could quantitatively detect AFB1 in wine and soy sauce samples via differential pulse voltammetry. The recovery rates of AFB1 in the samples were between 87.53% and 106.71%. The limit of detection of the biosensors was 0.016 pg mL−1. The electrochemical biosensors also had excellent sensitivity, reproducibility, specificity and stability. The synthetic strategy reported in this work could pave a new route to fabricate high-performance electrochemical biosensors for the detection of mycotoxins.

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Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes

Cited by 34 publications

References 36 publications

Horseradish peroxidase-triggered direct in situ fluorescent immunoassay platform for sensing cardiac troponin I and SARS-CoV-2 nucleocapsid protein in serum

Horseradish peroxidase-triggered direct in situ fluorescent immunoassay platform for sensing cardiac troponin I and SARS-CoV-2 nucleocapsid protein in serum

A Review on Cardiac Biomarkers Detection for Heart Failure Prognosis

A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1

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