Amperometric biosensor for NADH and ethanol based on electroreduced graphene oxide–polythionine nanocomposite film

Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials

“…A NADH biosensor has used the electro-deposition of ERGO and polythionine (PTH) on a GCE to form a nanocomposite film (Li et al, 2013c). This nanocomposite film was applied as a transducer to improve the electrocatalytic oxidation of NADH and also used to construct a dehydrogenase-based amperometric biosensor.…”

Preparation and electrochemical and electrochromic properties of wrinkled poly(N-methylthionine) film

“…Polythionine can be a conducting polymer as well as act as a redox mediator, which makes it very attractive [22][23][24][25]. It has a variety of promising potential and already realized applications in photogalvanic cells [26], fuel cells [27], capacitors [28], sensors [29], and biosensors [30].…”

An electrochemical biosensor based on DNA tetrahedron/graphene composite film for highly sensitive detection of NADH

“…Because of the cooperation of gold nanoparticles, DNA tetrahedrons and graphene fragments, there was an obvious oxidation peak of NADH at 0.28 V conducted with the composite film-modified electrode while there were less or no oxidation peaks aroused from the rest of electrodes invovled in the control experiments. Compared with the results reported with other graphene-modified electrodes, such as ionic liquid-functionalized graphene (IL-graphene) (0.33 V) (Shan et al, 2010), graphene-modified gold nanorods (0.4 V) (Li et al, 2013b) and electroreduced graphene oxide-polythionine nanocomposite film (0.4 V) (Li et al, 2013c), the NADH oxidation reaction occurred at a lower potential with the developed composited film-modified electrode.…”

“…As seen from Fig.3C, the lower detection limit of NADH by the composite film-modified electrode was reduced down to 1 fM, with a 10 pM upper limit. Compared to the detection limit of the graphene/nanostructures combined material mentioned above, such as graphene-Au nanorods nanocomposites (6.0 μM) (Li et al, 2013b), Fe 3 O 4 magnetic nanoparticles/reduced graphene oxide nanosheets (0.4 μM) (Teymourian et al, 2013) and electroreduced graphene oxide-polythionine nanocomposite film (0.1 μM) (Li et al, 2013c), the graphene-DNA tetrahedron-gold nanoparticle modified gold disk electrode exhibited a much lower detection limit of NADH.…”

“…For instance, Li et al created a sensitive NADH biosensor based on the combination of graphene and gold nanorods which decreased the oxidation potential of NADH to 0.4 V (Li et al, 2013b). Meanwhile, a nanocomposite film consisting of electroreduced graphene oxide and polythionine was also used to construct an amperometric biosensor on the surface of a glassy carbon electrode (GCE) and to determinate NADH at 0.4 V (Li et al, 2013c). Shan et al modified a GCE with ionic liquidfunctionalized graphene (IL-graphene) to decrease the oxidation potential of NADH to 0.33 V (Shan et al, 2010).…”