Amperometric Biosensors

Borgmann, Sabine; Schulte, Albert; Neugebauer, Sebastian; Schuhmann, Wolfgang

doi:10.1002/9783527644117.ch1

Cited by 65 publications

(56 citation statements)

References 390 publications

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“…Bio-sensing technology, for example an electrochemical approach such as that used by diabetics for glucose monitoring using WB [10,11] could offer a rapid, low cost alternative. An electrochemical method incorporating an antibody immobilised onto a screen-printed carbon electrode has been developed by our group for detection of picomolar concentrations of estradiol [12] and could be adapted for measurement of soluble markers in WB.…”

Section: Discussionmentioning

confidence: 99%

Use of whole blood for analysis of disease-associated biomarkers

May

Pemberton

Hart

et al. 2013

Analytical Biochemistry

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Section: Discussionmentioning

confidence: 99%

Use of whole blood for analysis of disease-associated biomarkers

May

Pemberton

Hart

et al. 2013

Analytical Biochemistry

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“…Amperometric biosensors depending on the ET mechanism can be classified according to three so-called 'generations' 49 ( Fig. 3.3), which will be illustrated by oxidases.…”

Section: Electron Transfer In Biosensorsmentioning

confidence: 99%

“…Even if the enzyme molecule is immobilised on the electrode surface, it can be oriented in such a way that direct ET is not possible due to a longer distance between the active site and the electrode compared to the ideal orientation. 49 Thus, although third-generation biosensors are advantageous compared to the two other generations, they are still not widely used due to aforementioned problems. DET transfer in biocatalytic systems will be overviewed in Chapter 5 of the thesis.…”

Section: "Third-generation" Biosensorsmentioning

confidence: 99%

Facilitating electron transfer in bioelectrocatalytic systems

Sekretaryova¹

2016

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During the course of the research underlying this thesis, Alina Sekretaryova was enrolled in Forum Scientium, a multidisciplinary doctoral programme at Linköping University, Sweden Alina Sekretaryova, 2016, unless otherwise noted. Cover design by Alina Sekretaryova Linköping studies in science and technology Dissertation No. 1738 Printed in Sweden by LiU-Tryck, Linköping, Sweden, 2016 iii ABSTRACT Bioelectrocatalytic systems are based on biological entities, such as enzymes, whole cells, parts of cells or tissues, which catalyse electrochemical processes that involve the interaction between chemical change and electrical energy. In all cases, enzymes, isolated or residing inside cells or part of cells, implement biocatalysis. Electron transfer phenomena, within the protein molecules and between biological redox systems and electronics, enable the development of various bioelectrocatalytic systems, which can be used both for fundamental investigations of enzymatic biological processes by electrochemical methods and for applied purposes, such as power generation, bioremediation, chemical synthesis and biosensing.Electrical communication between the biocatalysts redox centre and an electrode is essential for the functioning of the system. This can be established using two main mechanisms: indirect electron transfer and direct electron transfer. The efficiency of the electron transfer influences important parameters such as the turnover rate of the biocatalyst, the generated current density and partially the stability of the system. These in turn determine the response time, sensitivity, detection limit and operational stability of biosensors or the power densities and current output of biofuel cells, and hence they should be carefully considered when designing bioelectrocatalytic systems.This thesis focuses on approaches that facilitate electron transfer in bioelectrocatalytic systems based on mediated and direct electron transfer mechanisms. Both fundamental aspects of electron transfer in bioelectrocatalytic systems and applications of such systems for biosensing and power generation are considered. First, a new hydrophobic mediator for oxidases, unsubstituted phenothiazine, and its improved electron transfer properties in comparison with commonly used mediators are discussed. Application of the mediator in electrochemical biosensors is demonstrated by glucose, lactate and cholesterol sensing. Utilisation of mediated biocatalytic cholesterol oxidation as the anodic reaction for the construction of a biofuel cell, acting as a power supply and an analytical device at the same time, is investigated as a "self-powered" biosensor. In addition, the enhancement of mediated bioelectrocatalysis by the employment of microelectrodes as a transducer is examined. The effect of surface roughness on the current response of the microelectrodes under conditions of convergent diffusion is considered. The applicability of the laccase-based system for total phenol analysis of weakly supported water is demonstrated. Finally, a...

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“…Figure 5 depicts well-defined steps after each glucose adjunction for the polyhedron/Nafion ® catalyst. Especially at lower glucose concentration, the signal noise is small and the stages are well-formed (compare insets Figure 5a The limit of detection (LOD) was calculated by applying the 3-sigma method of the ground signal [31] in pure sodium hydroxide solution and using obtained sensitivities of all three types of catalysts. As a result, the LOD for the polyhedron/Nafion ® catalyst is 0.144 µmol .…”

Section: Amperometric Sensing Performance Of Glucosementioning

confidence: 99%

Facile wet-chemical synthesis of differently shaped cuprous oxide particles and a thin film: Effect of catalyst morphology on the glucose sensing performance

Neetzel

Muench

Matsutani

et al. 2015

Sensors and Actuators B: Chemical

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In this work, different facile synthesis routes were developed to create cuprite-based catalyst systems for the amperometric detection of glucose, allowing us to evaluate the impact of important electrode fabrication parameters on the glucose sensing performance. Using homogenous precipitation routes based on a redox system, two differently shaped cuprite particles-skeletons and polyhedrons-could be obtained. Furthermore, a novel electroless deposition technique was introduced that does not require sensitization and activation pretreatments, allowing for the direct modification of the glassy carbon. This technique produced electrodes with dense thin film consisting of merged, octahedral cuprite crystals. Afterward, these materials were tested as potential catalysts for the electrochemical detection of glucose. While the catalyst powders obtained by precipitation required Nafion ® to be attached to the electrode, the thin film synthesized using electroless plating could be realized with and without additive. Summarizing the results, it was found that Nafion ® was not required to achieve glucose selectivities typically observed for cuprite catalysts. Also, the type of catalyst application (direct plating versus ink drop coating) and the particle shape had a pronounced effect on the sensing performance. Compared to the thin film, the powder-type materials showed significantly increased electrochemical responses. The best overall performance was achieved with the polyhedral cuprite particles, resulting in a high sensitivity of 301 µA . mmol -1 cm -2 , a linear range up to 298 µmol . L -1 and a limit of detection of 0.144 µmol . L -1 .2

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Amperometric Biosensors

Cited by 65 publications

References 390 publications

Use of whole blood for analysis of disease-associated biomarkers

Use of whole blood for analysis of disease-associated biomarkers

Facilitating electron transfer in bioelectrocatalytic systems

Facile wet-chemical synthesis of differently shaped cuprous oxide particles and a thin film: Effect of catalyst morphology on the glucose sensing performance

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