Amperometric detection of salivary α-amylase on screen-printed carbon electrodes as a simple and inexpensive alternative for point-of-care testing

Garcia, Paulo de Tarso; Guimarães, Luiza Neves; Dias, Anderson A.; Ulhôa, Cirano José; Coltro, Wendell K. T.

doi:10.1016/j.snb.2017.11.068

Cited by 53 publications

(34 citation statements)

References 58 publications

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“…They detected indirectly sAA thanks to a sequence of two chemical reactions. The first reaction is the hydrolysis of starch to maltose by alpha-amylase, followed by the conversion of [Fe(CN) 6 ] 3− into [Fe(CN) 6 ] 4− due to the reducing sugar produced in the first step [ 131 ]. Many of the optimized devices can be used directly on the POC.…”

Section: Stress Related Biomarkersmentioning

confidence: 99%

“…Lastly, Tsyrulneva et al developed a simple, colorimetric method with a paper membrane strip that also can be used without a qualified analyst or expensive instrument [ 131 ]. They exploit the colored byproduct formed during the cleavage of the alpha-bond of 2-chloro-4-nitrophenyl-α-D-maltotrioside by sAA, to obtain a semi-quantitative analysis.…”

Section: Stress Related Biomarkersmentioning

confidence: 99%

“… 0.007 U/mL based on the tetraphenylethylene motif and g-cyclodextrin. Amperometry [ 131 ] n.s. 1.1 U/mL SPCE, indirect determination, POC Colorimetric Assay [ 135 ] [ 136 ] n.s.…”

Section: Stress Related Biomarkersmentioning

confidence: 99%

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Current methods for stress marker detection in saliva

Giacomello

Scholten

Parr

2020

Journal of Pharmaceutical and Biomedical Analysis

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Section: Stress Related Biomarkersmentioning

confidence: 99%

Section: Stress Related Biomarkersmentioning

confidence: 99%

See 1 more Smart Citation

Current methods for stress marker detection in saliva

Giacomello

Scholten

Parr

2020

Journal of Pharmaceutical and Biomedical Analysis

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“…One group of methods measures the consumption of natural starch hydrolyzed by α-amylase. Some examples of this approach include viscometries by capillary viscometer [7], magnetoelastic cantilever [8] or piezoelectric transducer [9,10], electrochemically amperometry [11,12,13,14,15,16], potentiometry [17], colorimetry of starch-iodine [18,19] or a chromogenic substrate [20] color intensity, and liquid crystals-based sensing platform [21]. These methods generally have poor reproducibility, with large differences associated with distinct operators or slight protocol variations, because the attack speed of α-amylase is deeply influenced by varying molecular mass and branching degrees of heterogeneous starch macromolecules [22].…”

Section: Introductionmentioning

confidence: 99%

Hand-held Colorimetry Sensor Platform for Determining Salivary α-Amylase Activity and Its Applications for Stress Assessment

Hsiao

Chen

Chou

et al. 2019

Sensors

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This study develops a hand-held stress assessment meter with a chemically colorimetric strip for determining salivary α-amylase activity, using a 3,5 dinitrosalicylic acid (DNS) assay to quantify the reducing sugar released from soluble starch via α-amylase hydrolysis. The colorimetric reaction is produced by heating the strip with a mini polyester heater plate at boiling temperature to form a brick red colored product, which measured at 525 nm wavelength. This investigation describes in detail the design, construction, and performance evaluation of a hand-held α-amylase activity colorimeter with a light emitted diode (LED) and photo-detector with built-in filters. The dimensions and mass of the proposed prototype are only 120 × 60 × 60 mm3 and 200 g, respectively. This prototype has an excellent correlation coefficient (>0.995), comparable with a commercial ultraviolet–visible spectroscope, and has a measurable α-amylase activity range of 0.1–1.0 U mL−1. The hand-held device can measure the salivary α-amylase activity with only 5 μL of saliva within 12 min of testing. This sensor platform effectively demonstrates that the level of salivary α-amylase activity increases more significantly than serum cortisol, the other physiological stressor biomarker, under physiologically stressful exercise conditions. Thus, this work demonstrates that the hand-held α-amylase activity meter is an easy to use and cost-effective stress assessment tool for psychoneuroendocrinology research.

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“…Also, screen-printed technology has become a preferred method for mass-produced biosensors for point-of-care testing (POCT). [33][34][35][36] In this study, β-CD-Fc-OH dissolved with ethylene glycol was mixed into carbon paste and printed on PET plates to form preliminary electrodes. And the creatininase, creatine oxidase, sarcosine oxidase and HRP were modified on preliminary electrodes to form ultimate electrodes (Fig.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Detection of Serum Creatinine Based on β-Cyclodextrin-Ferrocenylmethanol Modified Screen-printed Electrode

Liu

et al. 2019

ANAL. SCI.

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Ferrocenylmethanol (Fc-OH) is included in β-cyclodextrin (β-CD) to form the β-CD-Fc-OH complex by host-guest supramolecular interaction. β-CD dissociates from the β-CD-Fc-OH complex due to the conversion of Fc-OH to Fc +-OH under a stimulus of oxidant. In our study, Fc-OH is oxidized after a series of enzymatic reactions of creatinine, which blocks the other means for oxidation of Fc-OH. And the background noise is reduced for testing for serum creatinine (sCr). The chronoamperometry signal for creatinine (with a constant potential-0.3 V vs. Ag/AgCl) increases linearly in the 1-1000 μM range, with a limit of detection as low as 0.5 μM. The amperometric potential of-0.3 V greatly prevents the interference of various redox substances in serum. The biosensor was used to test 120 clinical specimens and the results showed a linear correlation with the biochemical analyzer (R 2 = 0.9885). The biosensor could be applied to clinical trials and offers good prospects for clinical sCr detection.

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Amperometric detection of salivary α-amylase on screen-printed carbon electrodes as a simple and inexpensive alternative for point-of-care testing

Cited by 53 publications

References 58 publications

Current methods for stress marker detection in saliva

Current methods for stress marker detection in saliva

Hand-held Colorimetry Sensor Platform for Determining Salivary α-Amylase Activity and Its Applications for Stress Assessment

Sensitive Detection of Serum Creatinine Based on β-Cyclodextrin-Ferrocenylmethanol Modified Screen-printed Electrode

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