Amperometric enzyme electrodes

An Amperometric Alcohol Sensor Based on Chemically Permeabilized Methylotrophic Microorganisms

“…The sensitivity decreased with any increase of the alcohol chain length. This behavior was also observed by other workers using purified alcohol oxidase enzyme (Guilbault et al, 1974;Nanjo et al, 1975;Sahm, 1976).…”

“…It has been reported that the activity of alcohol oxidase is rather low at pH values below 7.0 (Guilbault et al, 1974;Nanjo et al, 1975;Sahm, 1976). The study of the effect of pH on the response of our alcohol sensor was found to be different from that in other works.…”

“…The coupling of biochemical reactions with electrochemical electrodes for alcohol sensing has also received extensive attention in the literature. All kinds of isolated enzymes and microorganisms were used to construct alcohol biosensors: these included alcohol oxidase (Clark et al, 1972;Guilbault et al, 1974;Nanjo et al, 1975;Verduyn et al, 1983), alcohol dehydrogenase (Malinauskas et al, 1978;Blaedel et al, 1980;Walters et al, 1988; Kitagawa et al, 1989), and yeasts and bacteria (Hikuma et al, 1979;Kitagawa et al, 1987;Mascini et al, 1989).…”

Design, construction, and applications of a galactose selective electrode

“…Immobilized enzymes have a variety of applications in analytical chemistry (8). Specifically they have been employed in conjunction with electrochemical sensors to measure such substrates as glucose (9), urea (10), ethanol (11,12), uric acid (13), glutamine (14), etc. Both potentiometric and amperometric enzyme-electrodes have been reported as have the advantages and disadvantages of each (8).…”

Κατασκευη Ενος Νεου Αναλυτου Εγχυσης Σε Ροη (F.I.A) Και Χρηση Αμπερομετρικων Βιοαισθητηρων:μελετη Και Νεες Εφαρμογες Ενζυμικων Αντιδρασεων

“…Σε όλες τις περιπτώσεις το παραγόμενο ρεύμα είναι κατ ευθείαν ανάλογο της συγκέντρωσης της προσδιοριζόμενης ουσίας. Οι βιοαισθητήρες Η 2 0 2 είναι η πιο διαδεδομένη μορφή βιοαισθητήρων και μέχρι τώρα έχουν δημοσιευθεί πάνω από 1000 άρθρα καλύπτοντας έναν μεγάλο αριθμό αναλυτών όπως γλυκόζη [148], χοληστερόλη [149], αμινοξέα [150], αιθανόλη [151]. Διατίθενται στο εμπόριο από διάφορους οίκους όπως και Yellow Springs International (USA), Ependorf (Germany) , Fuji Electric (Japan), Tacussel (France), Radelkis (Hungary), La Roche (Switzerland), μοντέλο ExAn (πρώην Σοβιετική ένωση).…”