Amperometric flow-injection analysis of creatinine based on immobilized creatinine deiminase, leucine dehydrogenase and L-amino acid oxidase

Rui, Chang Sheng; Kato, Yasuhiko; Sonomoto, Kenji

doi:10.1016/0956-5663(94)90031-0

Cited by 7 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some other approaches used the pH-shift associated with the ammonia production for the determination of creatinine by potentiometric devices [32,33,34] or by AC impedance measurements [35]. Alternatively, the detection of oxygen consumption has been used by applying nitrifying bacteria [36,37] or coupling the deiminase reaction to a glutamate dehydrogenase/glutamate oxidase [38] or to a leucine dehydrogenase/L-amino acid oxidase [39] sequence. Recently a totally different principle, a competitive ELISA using an immobilized creatinine derivative as antigen, has been suggested to quantify creatinine concentrations [40].…”

Section: Introductionmentioning

confidence: 99%

Biosensors and flow-through system for the determination of creatinine in hemodialysate

Erlenkötter

Fobker

Chemnitius

2002

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

Biosensors for the determination of creatinine have been developed and integrated into a flow-through system. The sensors are based on a screen-printed three electrode transducer with a platinum working electrode. Applying the multi-enzyme sequence of creatininase (CA), creatinase (CI) and sarcosine oxidase (SO) hydrogen peroxide has been detected amperometrically. An optimal enzyme load was found to be 4.4 U/0.28 U/0.20 U (CA/CI/SO) and 0.28 U/0.20 U (CI/SO) per electrode for the creatinine sensor and for the creatine sensor, respectively. Among a variety of polymers Nafion has shown the highest efficiency to exclude interfering substances like ascorbic acid, acetaminophen and uric acid. First determinations of creatinine in dialysate samples obtained during hemodialysis treatments have shown a good correlation to the conventional methods, the Jaffé reaction (y=0.945x+ 2.8, R=0.9882, n=9) and the enzymatic photometric method (y=0.891x+3.5, R=0.9917, n=9).

show abstract

Section: Introductionmentioning

confidence: 99%

Biosensors and flow-through system for the determination of creatinine in hemodialysate

Erlenkötter

Fobker

Chemnitius

2002

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

show abstract

“…However, Jaffe method shows poor selectivity since it is affected by numerous metabolites containing carbonyl group found in biological samples, such as glucose, bilirubin and ascorbic acid [5,6]. To increase specificity, creatinine deiminase (CD) has been utilized to generate ammonia for amperometric detection though it is interfered from endogenous ammonia [7,8].…”

Section: Introductionmentioning

confidence: 99%

Amperometric biosensor based on reductive H2O2 detection using pentacyanoferrate-bound polymer for creatinine determination

Nieh

Tsujimura

Shirai

et al. 2013

Analytica Chimica Acta

View full text Add to dashboard Cite

Pentacyanoferrate-bound poly(1-vinylimidazole) (PVI[Fe(CN)5]) was selected as a mediator for amperometric creatinine determination based on the reductive H2O2 detection. Creatinine amidohydrolase (CNH), creatine amidohydrolase (CRH), sarcosine oxidase (SOD), peroxidase (POD), and PVI[Fe(CN)5] were crosslinked with poly(ethylene glycol) diglycidyl ether (PEGDGE) on a glassy carbon (GC) electrode for a creatinine biosensor fabrication. Reduction current was monitored at -0.1V in the presence of creatinine and O2. It is revealed that PVI[Fe(CN)5] is suitable as a mediator for a bioelectrocatalytic reaction of POD, since PVI[Fe(CN)5] neither reacts with reactants nor works as an electron acceptor of SOD. The amounts of PVI[Fe(CN)5], PEGDGE, and enzymes were optimized toward creatinine detection. Nafion as a protecting film successfully prevented the enzyme layer from interferences. The detection limit and linear range in creatinine determination were 12μM and 12-500μM (R(2)=0.993), respectively, and the sensitivity was 11mAcm(-2)M(-1), which is applicable for urine creatinine tests. The results of the creatinine determination for four urine samples measured with this proposed method were compared with Jaffe method, and a good correlation was obtained between the results.

show abstract

On-line ‘protein shaker’: A multicommutated flow analysis system for fluorometric creatinine determination in deproteinized serum

Malicka

Lewińska

Tymecki

2022

Analytica Chimica Acta

View full text Add to dashboard Cite

Amperometric flow-injection analysis of creatinine based on immobilized creatinine deiminase, leucine dehydrogenase and L-amino acid oxidase

Cited by 7 publications

References 12 publications

Biosensors and flow-through system for the determination of creatinine in hemodialysate

Biosensors and flow-through system for the determination of creatinine in hemodialysate

Amperometric biosensor based on reductive H2O2 detection using pentacyanoferrate-bound polymer for creatinine determination

On-line ‘protein shaker’: A multicommutated flow analysis system for fluorometric creatinine determination in deproteinized serum

Contact Info

Product

Resources

About