Amperometric Glucose Biosensor Based on Integration of Glucose Oxidase with Palladium Nanoparticles/Reduced Graphene Oxide Nanocomposite

Abstract

We report on a new type of amperometric glucose biosensor that was made by integration of glucose oxidase (GOD) with palladium nanoparticles/reduce graphene oxide (Pd/RGO) nanocomposite. The Pd/RGO was prepared by a onestep reduction method in which the palladium nanoparticles and the reduced graphene oxide (RGO) were simultaneously accomplished from the reduction of dispersed solution of PdCl 2 and graphite oxide (GO) with hydrazine. The as-prepared nanocomposite exhibits favorable electrocatalytic activities towards the oxidation of H 2 O 2 , which makes it a good platform for the construction of the glucose biosensor. The analytical performance of the glucose biosensor is fully evaluated. It shows good analytical properties in terms of a short response time (3 s), high sensitivity (14.1 µA/mM), and low detection limit (0.034 mM). In addition, the effects of pH value, applied potential, electroactive interference and the stability of the biosensor were discussed as well.

Fabrication of sensitive enzymatic biosensor based on multi-layered reduced graphene oxide added PtAu nanoparticles-modified hybrid electrode

“…Chemically modified graphite oxide (CGO) was synthesized using the modified Hummer methods [28, 29]. RGO was prepared using the following method.…”

Insight into the biosensing of graphene oxide: Present and future prospects

“…Thus, its detection in food samples is very important. The twodimensional (2D) honeycomb lattice of reduced GO holds a great potential for biosensor applications as it allows the fast movement of electrons for different electroactive species when employed as an electrode substrate (Cheng et al, 2012;Walcarius et al, 2013). GO based aptamers have now been developed for the detection and analysis of food borne pathogens.…”

Graphene nanosheets functionalized with Nile blue as a stable support for the oxidation of glucose and reduction of oxygen based on redox replacement of Pd-nanoparticles via nickel oxide

“…In Table 4 compare the performance of the modified electrodes with other Pd modified glucose sensors reported elsewhere [40][41][42][43][44][45][46]. As shown in Table 4, it is understandable that the linear calibration range and the applied potential of the new sensor is comparable and even better than those obtained by the other modified electrodes based on Pd nanoparticles, nanowires and nanocomposites.…”