Amperometric Glucose Biosensor Based on Pt‐Pd Nanoparticles Supported by Reduced Graphene Oxide and Integrated with Glucose Oxidase

Abstract

8 mM (R 2 = 0.997) and high sensitivity of 814 10 À6 A/ mMcm 2 . Furthermore, glucose oxidase with active material was integrated by a simple casting method on the RGO/PdPtNPs surface. The as-prepared biosensor showed good amperometric response to glucose in the linear range from 2 mM to 12 mM, with a sensitivity of 24 10 À6 A/mMcm 2 , a low detection limit of 0.001 mM, and a short response time (5 s). Moreover, the effect of interference materials, reproducibility and the stability of the sensor were also investigated.

Insight into the biosensing of graphene oxide: Present and future prospects

“…hybrid electrode had high catalytic activity towards the oxidation (response time of 3 s, sensitivity 486 x 10 -6 A/mMcm 2 , linear range 0.5-6.5 mM) and reduction (response time of 2 s, sensitivity 814 x 10 -6 A/mMcm 2 , linear range 0.5-8 mM) of hydrogen peroxide (Hossain and Park, 2014). The FTIR spectra of GO depicts major peaks at 3410.0 cm -1 , 1709.4 cm -1 , 1629.5 cm -1 , 1382.2 cm -1 and 1080.6 cm -1 , corresponding to -OH stretching, C=C stretching, -OH bending of absorbed water molecule, aromatic C=C, deformation vibration of -OH and C-O stretching vibration of alkoxy groups, respectively ( Figure 11A) (Khai et al, 2012;Shen et al, 2010).…”

Development of tyrosinase biosensor based on quantum dots/chitosan nanocomposite for detection of phenolic compounds

“…Since the discovery of carbon nanotubes, nanomaterials such as grapheme [13,14], magnetic nanobead [15], gold nanoparticle [16,17], polymer [18,19] and quantum dots [20,21] have received wide research interest in bioassays due to their electronic, optical, and mechanical properties. Among these nanomaterials, quantum dots (QDs) have been extensively used for optical and electrochemical biosensing because of their unique advantages such as nanoscale size similar to proteins, much higher specific surface, and versatility in surface modification, which are favorable for the immobilization of biomolecules [22][23][24][25][26].…”

Novel glucose sensor with Au@Ag heterogeneous nanorods based on electrocatalytic reduction of hydrogen peroxide at negative potential

“…For example, an enzyme based glucose sensor was developed by immobilizing glucose oxidase (GOx) in a poly (o-phenylenediamine) film, and a low response time was observed [12]. GOx was one of the most widely used enzymes in amperometric glucose sensors because of its high thermostability and good selectivity [13][14][15][16]. GOx could catalyze glucose oxidation to glucolactone in the aid of O 2 , resulting in the producing of H 2 O 2 .…”