Amperometric glucose sensor based on enzyme-modified boron-doped diamond electrode by cross-linking method

Amperometric biosensor based on tyrosinase immobilized on a boron-doped diamond electrode

“…Thus, the BDD electrodes are of interest for a variety of electrically based chemical and biological sensing applications (Haymond et al, 2002;Kawarada et al, 2001). However, to our knowledge, only a few reports exist on the development of the enzymatic sensors (Lee and Park, 2005;Rao et al, 1999;Su et al, 2004;Tatsuma et al, 2000), especially a tyrosinase-based sensor (Notsu et al, 2002) by using BDD electrode. The prevailing problem comes from the inertness of diamond film.…”

Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode

“…However, in the presence of glucose, anodic current began to flow at +0.15 V versus Ag/AgCl and was almost constant at +0.3 V versus Ag/AgCl or more positive potentials. This sigmoidal curve is typical of enzyme electrode reactions [11], indicating successful electron transfer from glucose to the BDD electrode. In the case of an electrode with a GOx/PPD film formed on the H-BDD surface (GOx/PPD-H-BDD), no current response was observed in the presence of glucose in the electrolyte solution ( Figure 6(b)).…”

“…Immobilization of particles or line patterns of these metals with micrometer or nanometer scale onto BDD surface enables sensitive glucose detection with low background current. On the other hand, BDD electrodes modified with glucose oxidase (GOx) [10][11][12][13] are advantageous because of their selectivity and sensitivity of the glucose molecule. In some cases, additional mediators, such as ferrocene carboxylic acid [11], were employed for glucose detection at GOx-modified BDD electrodes.…”

“…On the other hand, BDD electrodes modified with glucose oxidase (GOx) [10][11][12][13] are advantageous because of their selectivity and sensitivity of the glucose molecule. In some cases, additional mediators, such as ferrocene carboxylic acid [11], were employed for glucose detection at GOx-modified BDD electrodes. Glucose detection without any additional reagents to samples is possible for simple analysis systems, and especially for monitoring concentration.…”

Nanocrystalline diamond modified gold electrode for glucose biosensing

“…Hamers and coworkers employed nanocrystalline diamond thin films for DNA hybridization via photochemical modification of diamond surfaces, and showed that both as semiconductors, nanocrystalline diamond has superior properties over the crystalline silicon for the DNA hybridization (Yang et al, 2002;Strother et al, 2002Strother et al, , 2003. Proteins such as cytochrome c, antibodies and enzymes also have been immobilized on diamond films for biosensor applications (Lora Huang and Chang, 2004;Huang et al, 2004;Su et al, 2004). Since for electrochemical applications, the diamond thin film must possess a good conductivity, most work in this field has been carried out using diamond layers doped with boron (Levy-Clement et al, 1999), which display almost metal-like properties.…”