Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres

Abstract

Novel magnetic Fe3O4/chitosan (CS) microspheres were prepared using magnetic Fe3O4 nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin (Hb) immobilization method, an innovative biosensor with a Fe3O4/CS-Hb-Fe3O4/CS “sandwich” configuration was constructed. This biosensor had a fast (less than 10 s) response to H2O2 and excellent linear relationships were obtained in the concentration range of 5.0 × 10−5 to 1.8 × 10−3 M and 1.8 × 10−3 to 6.8 × 10−3 M with a detection limit of 4.0 × 10−6 M (s/n = 3) under the optimum conditions. The apparent Michaelis-Menten constant Km was 0.29 mM and it showed the excellent biological activity of the fixed Hb. Moreover, the biosensor had long-time stability and good reproducibility. The method was used to determine H2O2 concentration in real samples.

Direct electrochemistry of hemoglobin on graphene/Fe3O4 nanocomposite-modified glass carbon electrode and its sensitive detection for hydrogen peroxide

“…(3), k s can be estimated to be 0.3 s −1 . The estimated k s value is larger than that obtained for Hb immobilized on polymer-MWCNT-modified [9].…”

“…The detection limit of the biosensor was estimated at 6.0×10 −6 mol/L at a signal-to-noise ratio of 3. The linear ranger is broader than that of the Hb/MCMS/GCE [9]. The result indicates that the magnetic microspheres in the experiment displayed better catalytic ability than the microspheres using the magnetic core of carbon-coated iron nanoparticles.…”

“…Fe 3 O 4 with low toxicity, good biocompatibility, and strong adsorption ability may serve as an ideal matrix for investigating entrapped biomolecules. For these biomolecules, hemoglobin (Hb) is a heme protein containing four electroactive iron hemes, which can store and transport oxygen in red blood cells [9]. In contrast to most other proteins, Hb is highly stable and can construct H 2 O 2 sensor as a result of its commercial availability and peroxidase activity [10].…”

Non-enzymatic Reduction of Hydrogen Peroxide Sensor Based on (Polyaniline-polystyrene Sulphonate) - Carboxylated Graphene Modified Graphite Electrode

“…6b. The addition of H 2 O 2 to the stirred solution shows the increasing current response; however, the addition of interfering species such as AA (25 µM Table 2 with respect to operating conditions, sensitivity and the detection limit: it can be seen that the performance of the developed sensor is comparable to most of H 2 O 2 sensors reported in the literature [27][28][29][30][31][32]. …”

Cobalt nanoparticles as sacrificial templates for the electrodeposition of palladium nanomaterials in an ionic liquid, and its application to electrochemical sensing of hydrazine

“…3. Pt or platinized electrodes can lower the H 2 O 2 oxidation/reduction overvoltage [27] and they offer intrinsic sensitivity, extended dynamic range and rapid response time in electrochemical analyses and have thus been extensively employed for determination of H 2 O 2 . The cyclic voltammograms (CVs) are shown in the presence of 3.5 mmolL −1 (curve 3a) and 6 mmolL −1 H 2 O 2 (curve 3c) as well as oxygen flow for 5 min (curve 3b), respectively at the scan rate of 100 mV/s.…”